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Protein

Alpha-galactosidase

Gene

BF9343_4081

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationImported, Hydrolase

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-4161-MONOMER.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidaseUniRule annotation (EC:3.2.1.22UniRule annotation)
Alternative name(s):
MelibiaseUniRule annotation
Gene namesi
ORF Names:BF9343_4081Imported
OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)Imported
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000006731 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 496472Alpha-galactosidaseSequence analysisPRO_5004258694Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi215 ↔ 245Combined sources

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi272559.BF4189.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OGZX-ray2.00A/B25-496[»]
ProteinModelPortaliQ5L7T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 6429He_PIG_assocInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.UniRule annotation

Keywords - Domaini

SignalSequence analysis

Phylogenomic databases

eggNOGiENOG4105EX0. Bacteria.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiSQFITRV.
OrthoDBiEOG6N0HK6.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR019599. Alpha-galactosidase_NEW1.
IPR002241. Glyco_hydro_27.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF10632. He_PIG_assoc. 1 hit.
PF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5L7T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITSYVSFL YCLCLMLASP TVQASEVRTA IFEGKPCINP PHVVGNYPAT
60 70 80 90 100
PFLFYIPTSG ERPIKWHAEN LPKGLKLDKE TGIIKGKVVE KGTYKVMLKA
110 120 130 140 150
ENALGTDTQE LLINIGDELL LTPPMGWNSW NTFGRHLTEE LLLQTADAMV
160 170 180 190 200
ENGMRDLGYA YINIDDFWQL PERGADGHIQ IDKTKFPRGI KYVADYLHER
210 220 230 240 250
GFKLGIYSDA ADKTCGGVCG SYGYEEIDAR DFASWGVDLL KYDYCNAPAG
260 270 280 290 300
RVEAMERYEK MGRALRATDR SIVFSICEWG QREPWKWAKK VGGHLWRVSG
310 320 330 340 350
DIGDLWNRST DEKGGLRGIL NILEINAPLS EYARPGGWND PDMLVVGIGG
360 370 380 390 400
KSKSIGYESE GCTNEQYQSH FALWCMMASP LLCGNDVRQM NDSTLQILLN
410 420 430 440 450
KDLIAINQDP LGIQAERAIR ADHYDVWVKP LSDGSKAIAC LNRISGPVDV
460 470 480 490
ELNVKTVEGL SLDRVYDVIE GSLVAEASTG WVVKLAPGEC KVFICK
Length:496
Mass (Da):55,196
Last modified:June 21, 2005 - v1
Checksum:iEFCAD9802F91EA32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH09862.1.
RefSeqiWP_008770125.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH09862; CAH09862; BF9343_4081.
KEGGibfs:BF9343_4081.
PATRICi21045251. VBIBacFra29119_4307.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH09862.1.
RefSeqiWP_008770125.1. NC_003228.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OGZX-ray2.00A/B25-496[»]
ProteinModelPortaliQ5L7T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF4189.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Protocols and materials databases

DNASUi3287261.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH09862; CAH09862; BF9343_4081.
KEGGibfs:BF9343_4081.
PATRICi21045251. VBIBacFra29119_4307.

Phylogenomic databases

eggNOGiENOG4105EX0. Bacteria.
ENOG410XPF1. LUCA.
HOGENOMiHOG000161224.
KOiK07407.
OMAiSQFITRV.
OrthoDBiEOG6N0HK6.

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-4161-MONOMER.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR019599. Alpha-galactosidase_NEW1.
IPR002241. Glyco_hydro_27.
IPR013780. Glyco_hydro_b.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF10632. He_PIG_assoc. 1 hit.
PF16499. Melibiase_2. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343Imported.
  2. "Crystal structure of a putative alpha-galactosidase/melibiase (BF4189) from Bacteroides fragilis NCTC 9343 at 2.00 A resolution."
    JOINT CENTER FOR STRUCTURAL GENOMICS (JCSG)
    Submitted (JAN-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 25-496, DISULFIDE BONDS.

Entry informationi

Entry nameiQ5L7T7_BACFN
AccessioniPrimary (citable) accession number: Q5L7T7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 11, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.