Q5L7M8 (GLAA_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase A
EC=3.2.1.n1

Alternative name(s):
BfGal110A
Exo-alpha-galactosidase A
EC=3.2.1.22

Gene names

Name:	glaA
Ordered Locus Names:	BF4251

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase that specifically removes branched alpha-1,3-linked galactose residues present in blood group B antigens. Has no activity toward linear alpha-1,3-linked galactose residues.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.3 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. A subfamily. Contains 6 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 605	585	Alpha-1,3-galactosidase A		PRO_0000348471
Regions
	Repeat	256 – 278	23	PbH1 1
	Repeat	312 – 334	23	PbH1 2
	Repeat	421 – 443	23	PbH1 3
	Repeat	444 – 466	23	PbH1 4
	Repeat	477 – 507	31	PbH1 5
	Repeat	517 – 547	31	PbH1 6

Sequences

	Sequence		Length	Mass (Da)
	Q5L7M8 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: C57F2747FD520F43	FASTA	605	68,189
	10 20 30 40 50 60 MKKYLHILPA CFLFYAAAHA QQKDTVYVTD FGAVPYSYEN CVTQIQAAID ECKRTGAKVL 70 80 90 100 110 120 SLPEGRYDIW PEGAIRKEYY ISNTSTEQEC PSKVKTVGLM LHEIDDLTIE GNGATLMYHG 130 140 150 160 170 180 KMTTIALEHC NGVRINNLHI DFERPAGSEI QYRKVTGGET EVTLHRDTRY EIVNGKIRLY 190 200 210 220 230 240 GEGWRSNRNH CIEYDPDTES FTYSQGWNTL SASDAREIAP GIVRFNTPAE FMPKAGNTLT 250 260 270 280 290 300 VRDIIRDQVG LFILESKNIT LSRLQMHYMH GLGIVSQYTE NITMDRVKCA PRPDSGRLLA 310 320 330 340 350 360 ASADMMHFSG CKGKVIIDSC YFAGAQDDPV NVHGTNLRAL EKIDAQTLKL RFMHGQSYGF 370 380 390 400 410 420 NAYFKGDTVA FVRAATMERF ASATVRDVRR ISDRIVEVRF DRDIPTSLEL NHDCVENMTC 430 440 450 460 470 480 TPEVEIRNCY FTRTSTRGTL VTTPRKVVIE NNTYYKTGMS AILIEADAEG WYESGPVKDV 490 500 510 520 530 540 LIKGNTFIDC AYNGGPGHAV IAIHPSNKII DAERPVHQNI RIEDNTFRTF DYPVLYAKST 550 560 570 580 590 600 AGLLFRNNTI VRTETFPAVS GNPYVFYLNG CKKAVIEGTV FEGETPRQSI KTENMKRKDL KTTIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.
[3]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM109954 Genomic DNA. Translation: CAJ33350.1. CR626927 Genomic DNA. Translation: CAH09922.1.
RefSeq	YP_213813.1. NC_003228.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5L7M8.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3288075.
GenomeReviews	Gene locus BF4251 in contig CR626927_GR.
KEGG	bfs:BF4251.
PATRIC	21045381. VBIBacFra29119_4371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HBG345842.
OMA	LKLRFMH.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	BFRA272559:BF4251-MONOMER.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SMART	SM00710. PbH1. 6 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAA_BACFN

Accession

Primary (citable) accession number: Q5L7M8
Secondary accession number(s): A4Q8G5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program