Reviewed, UniProtKB/Swiss-Prot Q5L6B2 (MURE_CHLAB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CAB363

Organism

Chlamydophila abortus [Complete proteome] [HAMAP]

Taxonomic identifier

83555 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012343
Regions
	Nucleotide binding	109 – 115	7	ATP Potential
	Region	151 – 152	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	403 – 406	4	Meso-diaminopimelate binding By similarity
	Motif	403 – 406	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	178	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	380	1	Meso-diaminopimelate By similarity
	Binding site	453	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	457	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	218	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L6B2-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: DBD6F55D5E5DE792	FASTA	483	52,985
	10 20 30 40 50 60 MNLKELLHNT KAKIYGKISS VEVRNLTRDS RNVGVGDIFI AKQGKHCDGN DFSHLAVENG 70 80 90 100 110 120 AIAVASSIYN PFLPVVQIIS SDLPRLEADL AAKYYGHPSQ KLCVVGITGT NGKTTVSHLI 130 140 150 160 170 180 KLLFDACDKP AGLIGTIEHI LGNSRIQDGY TTPESCLLQK YLAKMVKSHL SAAVMEVSSI 190 200 210 220 230 240 GLAVNRLANV DFDVGVLTNL TLDHLDFHSS FEEYKQAKLK LFSMLPSSGL AVVNNDLCDA 250 260 270 280 290 300 AQFIEATQAQ PITYGIEQHA DYQASHVRFS PFGTDFDLLY KGETFACYSP LIGQHNIYNV 310 320 330 340 350 360 LAAIAVTHQR LRCDLPHLIS VIANVGAPRG RLEPIFSGPC PIYIDYAHTP DALDNVCQTL 370 380 390 400 410 420 QALLPQDGRL IVVFGCGGDR DQSKRKIMAQ VVEKYGFAVV TTDNPRGEDP EKIINEICSG 430 440 450 460 470 480 FLKRNFSIEI DRKQAITYAL SIASDRDIVL VAGKGHETYQ IFKHQTIAFD DKEIVLGVLS SYV

References

[1]

"The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation." Thomson N.R., Yeats C., Bell K., Holden M.T.G., Bentley S.D., Livingstone M., Cerdeno-Tarraga A.-M., Harris B., Doggett J., Ormond D., Mungall K., Clarke K., Feltwell T., Hance Z., Sanders M., Quail M.A., Price C., Barrell B.G., Parkhill J., Longbottom D. Genome Res. 15:629-640(2005) [PubMed: 15837807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S26/3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848038 Genomic DNA. Translation: CAH63816.1.
RefSeq	YP_219780.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8C based on UniProtKB P22188.
SMR	Q5L6B2. Positions 2-481.
ModBase	Search...
Genome annotation databases
GeneID	3337461.
GenomeReviews	Gene locus CAB363 in contig CR848038_GR.
KEGG	cab:CAB363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG602753.
OMA	NPRSENE.
PhylomeDB	Q5L6B2.
Enzyme and pathway databases
BioCyc	CABO218497:CAB363-MONOMER.
BRENDA	6.3.2.13. 292247.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_CHLAB

Accession

Primary (citable) accession number: Q5L6B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)