Q5L681 (AMPA_CHLAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	CAB397

Organism

Chlamydophila abortus [Complete proteome] [HAMAP]

Taxonomic identifier

83555 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019903
Sites
	Active site	276	1	Potential
	Active site	350	1	Potential
	Metal binding	264	1	Manganese 2 By similarity
	Metal binding	269	1	Manganese 1 By similarity
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	287	1	Manganese 2 By similarity
	Metal binding	346	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L681 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 5377B21CC9C1812A	FASTA	500	54,457
	10 20 30 40 50 60 MVLFHSQASC SKRVKADAIV LPFWKVKSKP KCAASIAKEY ESLYRVALDS FSGERGEVEF 70 80 90 100 110 120 IYNSGQGKEK RLLILGLGKN EELTSQDVLE VYAKVTRTLR KAKCTTVNVV LPTISELRIP 130 140 150 160 170 180 VEDFLTNLTS GILSLNYNYP KYTKEAKKTD PLLTKVTVLG IVPKIADRIF RKEESIFEGV 190 200 210 220 230 240 YLTRDLVNGN ADEVTPEKLA NIAKGLAKQF PSVDAKVLNK DAILKEKMGL LAAVAKGSAV 250 260 270 280 290 300 DPRFIVLSYQ GKPKSKDHTV LIGKGVTFDS GGLDLKPGKA MLTMKEDMAG AATVLGILSG 310 320 330 340 350 360 VAALELPVNV TALIPATENA IDAASYKMGD VYVGMSGLSV EIGSTDAEGR LILADAITYA 370 380 390 400 410 420 LKYCKPTRII DFATLTGAMV VSLGEDVAGF FSNNDVLAQD LFEASAETSE SLWRLPLVEK 430 440 450 460 470 480 YDKALHSDIA DMKNIGSNRA GAITAALFLK RFLEDQPVAW AHLDIAGTAY REKDEDAYPK 490 500 YASGFGVRCL IYYIEKFLSK

References

[1]

"The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation." Thomson N.R., Yeats C., Bell K., Holden M.T.G., Bentley S.D., Livingstone M., Cerdeno-Tarraga A.-M., Harris B., Doggett J., Ormond D., Mungall K., Clarke K., Feltwell T., Hance Z., Sanders M., Quail M.A., Price C., Barrell B.G., Parkhill J., Longbottom D. Genome Res. 15:629-640(2005) [PubMed: 15837807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S26/3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848038 Genomic DNA. Translation: CAH63850.1.
RefSeq	YP_219811.1. NC_004552.2.
3D structure databases
HSSP	HSSP built from PDB template 1LAP based on UniProtKB P00727.
ProteinModelPortal	Q5L681.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3337398.
GenomeReviews	Gene locus CAB397 in contig CR848038_GR.
KEGG	cab:CAB397.
PATRIC	20202263. VBIChlAbo21869_0448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	CKPTRII.
PhylomeDB	Q5L681.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	CABO218497:CAB397-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CHLAB

Accession

Primary (citable) accession number: Q5L681

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program