Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydophila abortus (strain DSM 27085 / S26/3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei595 – 5951ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCABO218497:GJB0-650-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CAB634
OrganismiChlamydophila abortus (strain DSM 27085 / S26/3)
Taxonomic identifieri218497 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydophila
ProteomesiUP000001012 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10431043Isoleucine--tRNA ligasePRO_0000098530Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218497.CAB634.

Structurei

3D structure databases

ProteinModelPortaliQ5L5L0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi592 – 5965"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiREGCEEF.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L5L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTEGEGSKE SLASREERIL NFWKTQEIFQ KSLKNREGRT LYSFYDGPPF
60 70 80 90 100
ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS
110 120 130 140 150
LNLTTPGAIE DFGVAKFNEE CRKIVFRYVD EWEHYIYRVG RWVDFSATWK
160 170 180 190 200
TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK
210 220 230 240 250
EVDDPSVVIK FALHGDPGSL LVWTTTPWTL VSNMAVAVGP EITYVRVADK
260 270 280 290 300
VSGEQWILGQ GCLSRWFSDP DTYEVIESFP GTALIGKSYE PPFNFFEQKR
310 320 330 340 350
AEGAYTILPG SFVEESEGTG VVHMAPAFGE ADFFVCKEHH VPMVCPVDNH
360 370 380 390 400
GCFTEEIPEY QGQYIKSCDK GIIKSLKNQG KVFYHGTVVH RYPFCWRTDT
410 420 430 440 450
PLIYKTVNSW FISVEKIKDK MLQANKKIHW VPEHIKEGRF GKWLEGARDW
460 470 480 490 500
AISRNRYWGT PIPVWKSKDG DILVIGSVEE LEKLTGEKVS DLHCHFVDQL
510 520 530 540 550
KIEKDGKSFH RVPYVFDCWF DSGAMPYAQN HYPFENQKET ESGFPADFIA
560 570 580 590 600
EGLDQTRGWF YTLTVISAAL FDQPVFKNAI VNGIVLAEDG NKMSKRLNNY
610 620 630 640 650
PSPMSIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESV LKQVLLPLTN
660 670 680 690 700
VLSFFKTYTD LYGFDANNYD KEEISYSEID RWILSNLYTV VGKVRESMSS
710 720 730 740 750
YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESADTPDRRA AFATLYEVLT
760 770 780 790 800
VFCRVIAPFI PFISEDIYQQ IKTENSLESV HLCDFPYIDL AKVFPDLEQR
810 820 830 840 850
MGDAREIVGL GHSLRKEHKL KVRQPLANFY VVGPKDRLDQ LDSFKQLISE
860 870 880 890 900
ELNVKNIVFY KEAPSFVKTT VKPNFRSLGR RVGEKIKDIQ KALASLSQAQ
910 920 930 940 950
IQQLLTQEYL SLNLGSEEIV LHMEDVLISW ETDPGYVARS SSLFTVVLDC
960 970 980 990 1000
QLTEELVVEA ISRELVNKIN TMRRNQKLHV SDRIVLRMQT SEEVRKAFLH
1010 1020 1030 1040
YADYICEETL TTQSEFADVL EGEEWDINGH PTVIAIEVAA RPH
Length:1,043
Mass (Da):119,728
Last modified:June 21, 2005 - v1
Checksum:i331B040D1C6F5114
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR848038 Genomic DNA. Translation: CAH64081.1.
RefSeqiWP_011097220.1. NC_004552.2.

Genome annotation databases

EnsemblBacteriaiCAH64081; CAH64081; CAB634.
KEGGicab:CAB634.
PATRICi20202779. VBIChlAbo21869_0696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR848038 Genomic DNA. Translation: CAH64081.1.
RefSeqiWP_011097220.1. NC_004552.2.

3D structure databases

ProteinModelPortaliQ5L5L0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218497.CAB634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH64081; CAH64081; CAB634.
KEGGicab:CAB634.
PATRICi20202779. VBIChlAbo21869_0696.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiREGCEEF.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCABO218497:GJB0-650-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 27085 / S26/3.

Entry informationi

Entry nameiSYI_CHLAB
AccessioniPrimary (citable) accession number: Q5L5L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 21, 2005
Last modified: July 22, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.