Q5L5F7 (BIOD_CHLAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CAB687

Organism

Chlamydophila abortus [Complete proteome] [HAMAP]

Taxonomic identifier

83555 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydophila

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_1000133208
Regions
	Nucleotide binding	95 – 98	4	ATP By similarity
	Nucleotide binding	155 – 156	2	ATP By similarity
Sites
	Metal binding	11	1	Magnesium 1 By similarity
	Metal binding	15	1	Magnesium 2 By similarity
	Metal binding	42	1	Magnesium 2 By similarity
	Metal binding	95	1	Magnesium 2 By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	42	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L5F7 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 2794D7A5FE79FE15	FASTA	207	23,165
	10 20 30 40 50 60 MHIIIAGIDT DVGKTFVSAI LTVLLQAEYW KPIQSGSLNH SDSAIVHALS GARCHPESYR 70 80 90 100 110 120 FSHALAAHQA AQIDNITMHQ ETITLPKTDA SLIIETSGGF LSPCTHDSLQ GDVFAQWPCH 130 140 150 160 170 180 WVLVSKAYLG SINHTCLTLE AMRARNLSIL GIILNQYPKE EEDWLLRTTG LPFLGRLNYE 190 200 KSISKATVQN YANLWKETWK YRDTLLC

References

[1]

"The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation." Thomson N.R., Yeats C., Bell K., Holden M.T.G., Bentley S.D., Livingstone M., Cerdeno-Tarraga A.-M., Harris B., Doggett J., Ormond D., Mungall K., Clarke K., Feltwell T., Hance Z., Sanders M., Quail M.A., Price C., Barrell B.G., Parkhill J., Longbottom D. Genome Res. 15:629-640(2005) [PubMed: 15837807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S26/3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848038 Genomic DNA. Translation: CAH64134.1.
RefSeq	YP_220085.1. NC_004552.2.
3D structure databases
ProteinModelPortal	Q5L5F7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3337274.
GenomeReviews	Gene locus CAB687 in contig CR848038_GR.
KEGG	cab:CAB687.
PATRIC	20202897. VBIChlAbo21869_0752.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	CLSK399497.
Enzyme and pathway databases
BioCyc	CABO218497:CAB687-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CHLAB

Accession

Primary (citable) accession number: Q5L5F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program