Reviewed, UniProtKB/Swiss-Prot Q5L5A3 (MURB_CHLAB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CAB741

Organism

Chlamydophila abortus [Complete proteome] [HAMAP]

Taxonomic identifier

83555 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224676
Regions
	Domain	26 – 191	166	FAD-binding PCMH-type
Sites
	Active site	170	1	By similarity
	Active site	218	1	Proton donor By similarity
	Active site	287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L5A3-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 2FC10FD71EA60A1D	FASTA	296	32,373
	10 20 30 40 50 60 MKASTVIRFP FSVRRSVWLN KYSTFRIGGP ANYFKVVHSA SEAQQVIQFL HSHNYPFIIV 70 80 90 100 110 120 GKGSNCLFDD RGFDGFVLCN GIQGKEFVSE TTIKVYSGTS FSFLGKTLSS SGYSGLEFAV 130 140 150 160 170 180 GIPGSVGGAV FMNAGIGNQD TAAVIESVEV INSKGEILSY NTEELGFGYR TSRFQHCNEF 190 200 210 220 230 240 ILSATFRLSR NSSSVKIAKD LLRNRLLSQP YQQPSTGCIF RNPPGNSAGK LIDEAGLKGF 250 260 270 280 290 SLGGAQISPK HANFIVNTGR ATSQEVKQLI QIVQDKLKSQ GINLEEEVRI IPYQLP

References

[1]

"The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies variation." Thomson N.R., Yeats C., Bell K., Holden M.T.G., Bentley S.D., Livingstone M., Cerdeno-Tarraga A.-M., Harris B., Doggett J., Ormond D., Mungall K., Clarke K., Feltwell T., Hance Z., Sanders M., Quail M.A., Price C., Barrell B.G., Parkhill J., Longbottom D. Genome Res. 15:629-640(2005) [PubMed: 15837807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: S26/3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR848038 Genomic DNA. Translation: CAH64188.1.
RefSeq	YP_220139.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3337855.
GenomeReviews	Gene locus CAB741 in contig CR848038_GR.
KEGG	cab:CAB741.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L5A3.
OMA	GPADILI.
Enzyme and pathway databases
BioCyc	CABO218497:CAB741-MON.
BRENDA	1.1.1.158. 292247.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CHLAB

Accession

Primary (citable) accession number: Q5L5A3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)