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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 31ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processtRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.19 8138

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:GK0060
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816961 – 464tRNA(Ile)-lysidine synthaseAdd BLAST464

Proteomic databases

PRIDEiQ5L3T3

Interactioni

Protein-protein interaction databases

DIPiDIP-59287N
STRINGi235909.GK0060

Structurei

Secondary structure

1464
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi338 – 341Combined sources4
Beta strandi343 – 348Combined sources6
Beta strandi352 – 362Combined sources11
Beta strandi372 – 375Combined sources4
Helixi377 – 379Combined sources3
Beta strandi382 – 387Combined sources6
Beta strandi394 – 398Combined sources5
Beta strandi403 – 405Combined sources3
Helixi406 – 412Combined sources7
Helixi417 – 420Combined sources4
Beta strandi425 – 427Combined sources3
Beta strandi433 – 436Combined sources4
Turni437 – 439Combined sources3
Beta strandi440 – 442Combined sources3
Beta strandi454 – 462Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A2KX-ray3.65A/B1-464[»]
3HJ7X-ray2.20A332-464[»]
ProteinModelPortaliQ5L3T3
SMRiQ5L3T3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L3T3

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U Bacteria
COG0037 LUCA
HOGENOMiHOG000236506
KOiK04075
OMAiAHCNFNL
OrthoDBiPOG091H02FP

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
IPR015262 tRNA_Ile_lys_synt_subst-bd
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF09179 TilS, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q5L3T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDKVRAFIH RHQLLSEGAA VIVGVSGGPD SLALLHVFLS LRDEWKLQVI
60 70 80 90 100
AAHVDHMFRG RESEEEMEFV KRFCVERRIL CETAQIDVPA FQRSAGLGAQ
110 120 130 140 150
EAARICRYRF FAELMEKHQA GYVAVGHHGD DQVETILMRL VRGSTSKGYA
160 170 180 190 200
GIPVKRPFHG GYLIRPFLAV SRAEIEAYCR QMGLSPRCDP SNEKDDYTRN
210 220 230 240 250
RFRHHIVPLL RQENPRLHER FQQYSEMMAE DEQFLEELAA DALNKVMEKQ
260 270 280 290 300
HRDAALSIGP FLELPRPLQR RVLQLLLLRL YGGVPPTLTS VHIGHILMLC
310 320 330 340 350
ERGRPSGMID LPKGLKVIRS YDRCLFTFDA ESGEKGYWFE LPVPALLPLP
360 370 380 390 400
NGYAIISEFG EHYPRKQAGN DWFVVDPASV SLPLRVRTRR RGDRMVLKGT
410 420 430 440 450
GGTKKLKEIF IEAKIPRMER DRWPIVEDAD GRILWVPGLK KSAFEAQNRG
460
QARYILLQYQ AMNS
Length:464
Mass (Da):53,105
Last modified:February 1, 2005 - v1
Checksum:i6C81D0B26D2FFCFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA Translation: BAD74345.1
RefSeqiWP_011229575.1, NC_006510.1

Genome annotation databases

EnsemblBacteriaiBAD74345; BAD74345; GK0060
KEGGigka:GK0060

Similar proteinsi

Entry informationi

Entry nameiTILS_GEOKA
AccessioniPrimary (citable) accession number: Q5L3T3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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