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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotation

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 31ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.4.19. 8138.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotation (EC:6.3.4.19UniRule annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
Ordered Locus Names:GK0060
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001816961 – 464tRNA(Ile)-lysidine synthaseAdd BLAST464

Interactioni

Protein-protein interaction databases

DIPiDIP-59287N.
STRINGi235909.GK0060.

Structurei

Secondary structure

1464
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi338 – 341Combined sources4
Beta strandi343 – 348Combined sources6
Beta strandi352 – 362Combined sources11
Beta strandi372 – 375Combined sources4
Helixi377 – 379Combined sources3
Beta strandi382 – 387Combined sources6
Beta strandi394 – 398Combined sources5
Beta strandi403 – 405Combined sources3
Helixi406 – 412Combined sources7
Helixi417 – 420Combined sources4
Beta strandi425 – 427Combined sources3
Beta strandi433 – 436Combined sources4
Turni437 – 439Combined sources3
Beta strandi440 – 442Combined sources3
Beta strandi454 – 462Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A2KX-ray3.65A/B1-464[»]
3HJ7X-ray2.20A332-464[»]
ProteinModelPortaliQ5L3T3.
SMRiQ5L3T3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L3T3.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiPOG091H02FP.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5L3T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDKVRAFIH RHQLLSEGAA VIVGVSGGPD SLALLHVFLS LRDEWKLQVI
60 70 80 90 100
AAHVDHMFRG RESEEEMEFV KRFCVERRIL CETAQIDVPA FQRSAGLGAQ
110 120 130 140 150
EAARICRYRF FAELMEKHQA GYVAVGHHGD DQVETILMRL VRGSTSKGYA
160 170 180 190 200
GIPVKRPFHG GYLIRPFLAV SRAEIEAYCR QMGLSPRCDP SNEKDDYTRN
210 220 230 240 250
RFRHHIVPLL RQENPRLHER FQQYSEMMAE DEQFLEELAA DALNKVMEKQ
260 270 280 290 300
HRDAALSIGP FLELPRPLQR RVLQLLLLRL YGGVPPTLTS VHIGHILMLC
310 320 330 340 350
ERGRPSGMID LPKGLKVIRS YDRCLFTFDA ESGEKGYWFE LPVPALLPLP
360 370 380 390 400
NGYAIISEFG EHYPRKQAGN DWFVVDPASV SLPLRVRTRR RGDRMVLKGT
410 420 430 440 450
GGTKKLKEIF IEAKIPRMER DRWPIVEDAD GRILWVPGLK KSAFEAQNRG
460
QARYILLQYQ AMNS
Length:464
Mass (Da):53,105
Last modified:February 1, 2005 - v1
Checksum:i6C81D0B26D2FFCFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74345.1.
RefSeqiWP_011229575.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD74345; BAD74345; GK0060.
GeneIDi3183848.
KEGGigka:GK0060.
PATRICi21961149. VBIGeoKau81518_0110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74345.1.
RefSeqiWP_011229575.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A2KX-ray3.65A/B1-464[»]
3HJ7X-ray2.20A332-464[»]
ProteinModelPortaliQ5L3T3.
SMRiQ5L3T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59287N.
STRINGi235909.GK0060.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD74345; BAD74345; GK0060.
GeneIDi3183848.
KEGGigka:GK0060.
PATRICi21961149. VBIGeoKau81518_0110.

Phylogenomic databases

eggNOGiENOG4105D3U. Bacteria.
COG0037. LUCA.
HOGENOMiHOG000236506.
KOiK04075.
OMAiNDISYPL.
OrthoDBiPOG091H02FP.

Enzyme and pathway databases

BRENDAi6.3.4.19. 8138.

Miscellaneous databases

EvolutionaryTraceiQ5L3T3.

Family and domain databases

CDDicd01992. PP-ATPase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt. 1 hit.
InterProiIPR012796. Lysidine-tRNA-synth_C.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. TilS/TtcA_N.
IPR012094. tRNA_Ile_lys_synt.
IPR012795. tRNA_Ile_lys_synt_N.
IPR015262. tRNA_Ile_lys_synt_subst-bd.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF09179. TilS. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTILS_GEOKA
AccessioniPrimary (citable) accession number: Q5L3T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.