Reviewed, UniProtKB/Swiss-Prot Q5L3K8 (ROCA_GEOKA)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	GK0187

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_1000045970
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L3K8-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 9E3253E0D5EB852E	FASTA	515	56,667
	10 20 30 40 50 60 MVQPYRHEPL TDFTVEANRE AFLAALKKVE SELGRDYPLV IGGERVMTED KIISINPANK 70 80 90 100 110 120 TEVVGRVAKA NKELAERAMK TADEAFRTWS RTSPEARADI LFRAAAIVRR RKHEFSAWLV 130 140 150 160 170 180 KEAGKPWREA DADTAEAIDF MEYYGRQMLK LKDGIPVESR PGETNRFFYI PLGVGVVISP 190 200 210 220 230 240 WNFPFAIMAG TTVASLVTGN TVLLKPASAT PVVAYKFVEV LEEAGLPAGV LNYIPGSGAE 250 260 270 280 290 300 VGDYLVDHPR TRFISFTGSR DVGIRIYERA AKVHPGQIWL KRVIAEMGGK DAIVVDKEAD 310 320 330 340 350 360 LELAAQSIVA SAFGFSGQKC SACSRAIIVE DVYDQVLSRV VELTKQLNVG DPAEQATFMG 370 380 390 400 410 420 PVIDQNAYNK IMEYIEIGKQ EGRLMTGGEG DDAKGFFIQP TVFADVDPNA RIMQEEIFGP 430 440 450 460 470 480 VVAFAKARDF DHALEIANNT EYGLTGAVIS RNRANLEKAR HEFHVGNLYF NRGCTGAIVG 490 500 510 YQPFGGFNMS GTDSKAGGPD YLILHMQAKT VSEMF

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD74472.1.
RefSeq	YP_146040.1.
3D structure databases
HSSP	HSSP built from PDB template 2BJK based on UniProtKB Q5SI02.
SMR	Q5L3K8. Positions 2-515.
ModBase	Search...
Genome annotation databases
GeneID	3186084.
GenomeReviews	Gene locus GK0187 in contig BA000043_GR.
KEGG	gka:GK0187.
NMPDR	fig|235909.3.peg.1485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	TPYKHEP.
Enzyme and pathway databases
BioCyc	GKAU235909:GK0187-MONOMER.
BRENDA	1.5.1.12. 281547.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_GEOKA

Accession

Primary (citable) accession number: Q5L3K8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)