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Q5L3H2 (DDL_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:GK0223
OrganismGeobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]
Taxonomic identifier235909 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030449

Regions

Domain141 – 346206ATP-grasp
Nucleotide binding174 – 22956ATP By similarity

Sites

Metal binding3001Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 1 By similarity
Metal binding3131Magnesium or manganese 2 By similarity
Metal binding3151Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5L3H2 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: FB581628BA66B0F6

FASTA36740,687
        10         20         30         40         50         60 
MKTKVGVLYG GKSPEHQVSL STAMAVMNAI DPHKFDVIPI YITPEGQWIK GEQLTGPIEE 

        70         80         90        100        110        120 
IKQLQFTSAA TALIPVSLNQ VPAADSAAGG NEETIDVIFP LLHGPNGEDG TVQGLLEILN 

       130        140        150        160        170        180 
IPYVGNGVLA SAVGMDKVMM KNLFAQAGLR QAKYIAVTKY DWQKRGETVY DRIERELGYP 

       190        200        210        220        230        240 
CFVKPANAGS SVGISKCKQR GDLKAAFIEA FQYDRKIIIE EAIVGREIEI GVIGNDEPIC 

       250        260        270        280        290        300 
SVVGEIVPKK EFYDYEAKYE DGQTELIIPA DVTKEQYETI KQMAITAFQV LDLSGLARVD 

       310        320        330        340        350        360 
FFLAEDGAVY INEVNTMPGF TPYSMFPLLW QHSGVPYPEL IERLIALALE RHQEKQTITY 


TFKKEKR 

« Hide

References

[1]"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTA426.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000043 Genomic DNA. Translation: BAD74508.1.
RefSeqYP_146076.1. NC_006510.1.

3D structure databases

ProteinModelPortalQ5L3H2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING235909.GK0223.

Proteomic databases

PRIDEQ5L3H2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD74508; BAD74508; GK0223.
GeneID3185538.
KEGGgka:GK0223.
PATRIC21961539. VBIGeoKau81518_0283.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAWREAARY.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycGKAU235909:GJO7-257-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_GEOKA
AccessionPrimary (citable) accession number: Q5L3H2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 2005
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways