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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

purC

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.UniRule annotationSAAS annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC)
  2. Adenylosuccinate lyase (GK0259)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241ADPCombined sources
Binding sitei180 – 1801ADPCombined sources
Binding sitei193 – 1931ADPCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146ADPCombined sources
Nucleotide bindingi83 – 853ADPCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotationImported

Keywords - Biological processi

Purine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-311-MONOMER.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthaseUniRule annotationSAAS annotation (EC:6.3.2.6UniRule annotationSAAS annotation)
Alternative name(s):
SAICAR synthetaseUniRule annotation
Gene namesi
Name:purCUniRule annotation
Ordered Locus Names:GK0260Imported
OrganismiGeobacillus kaustophilus (strain HTA426)Imported
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi235909.GK0260.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YWVX-ray1.75A/B1-242[»]
ProteinModelPortaliQ5L3D5.
SMRiQ5L3D5. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L3D5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 230224SAICAR_syntInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the SAICAR synthetase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000082629.
KOiK01923.
OMAiFNAQKRG.
OrthoDBiEOG62C9HN.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L3D5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTKQQLLYE GKAKKIYATD EPDVLWVEYK DSATAFNGEK KATIAGKGRL
60 70 80 90 100
NNEISSLLFL KLREAGIANH FIEKLSPTEQ LVRRVTIIPL EVVVRNVVAG
110 120 130 140 150
SLAKRIGLEE GTPLEAPLVE FYYKNDDLGD PLLLEDHIFI LKLASREEVA
160 170 180 190 200
ALKQAALAVN DVLRLHFAER NVRLIDFKLE FGRTADGAIL LADEISPDTC
210 220 230 240
RLWDAKTNEK LDKDVFRRDL GSLTDAYEVI LQRLGGESAC TK
Length:242
Mass (Da):27,095
Last modified:February 1, 2005 - v1
Checksum:i74CEA823D1DD7F5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74545.1.

Genome annotation databases

EnsemblBacteriaiBAD74545; BAD74545; GK0260.
KEGGigka:GK0260.
PATRICi21961664. VBIGeoKau81518_0328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74545.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YWVX-ray1.75A/B1-242[»]
ProteinModelPortaliQ5L3D5.
SMRiQ5L3D5. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK0260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD74545; BAD74545; GK0260.
KEGGigka:GK0260.
PATRICi21961664. VBIGeoKau81518_0328.

Phylogenomic databases

eggNOGiENOG4105C8V. Bacteria.
COG0152. LUCA.
HOGENOMiHOG000082629.
KOiK01923.
OMAiFNAQKRG.
OrthoDBiEOG62C9HN.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00131.
BioCyciGKAU235909:GJO7-311-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5L3D5.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426Imported.
  2. "Crystal structure of SAICAR synthetase from Geobacillus kaustophilus."
    Kanagawa M., Baba S., Kuramitsu S., Yokoyama S., Kawai G., Sampei G.
    Submitted (APR-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) IN COMPLEX WITH ADP.

Entry informationi

Entry nameiQ5L3D5_GEOKA
AccessioniPrimary (citable) accession number: Q5L3D5
Entry historyi
Integrated into UniProtKB/TrEMBL: February 1, 2005
Last sequence update: February 1, 2005
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.