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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligaseUniRule annotation (EC:6.3.3.1UniRule annotation)
Alternative name(s):
AIR synthaseUniRule annotation
AIRSUniRule annotation
Phosphoribosyl-aminoimidazole synthetaseUniRule annotation
Gene namesi
Name:purMUniRule annotation
Ordered Locus Names:GK0265
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002583561 – 346Phosphoribosylformylglycinamidine cyclo-ligaseAdd BLAST346

Interactioni

Protein-protein interaction databases

STRINGi235909.GK0265.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi18 – 29Combined sources12
Helixi47 – 50Combined sources4
Beta strandi53 – 63Combined sources11
Helixi67 – 74Combined sources8
Helixi80 – 93Combined sources14
Turni94 – 96Combined sources3
Beta strandi98 – 110Combined sources13
Helixi113 – 130Combined sources18
Beta strandi133 – 136Combined sources4
Beta strandi151 – 162Combined sources12
Helixi163 – 165Combined sources3
Beta strandi177 – 182Combined sources6
Beta strandi184 – 186Combined sources3
Helixi191 – 199Combined sources9
Helixi217 – 222Combined sources6
Helixi229 – 238Combined sources10
Beta strandi243 – 246Combined sources4
Helixi251 – 259Combined sources9
Beta strandi264 – 269Combined sources6
Helixi277 – 285Combined sources9
Helixi290 – 296Combined sources7
Beta strandi301 – 307Combined sources7
Helixi309 – 311Combined sources3
Helixi312 – 321Combined sources10
Beta strandi327 – 341Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Z01X-ray2.20A1-346[»]
ProteinModelPortaliQ5L3D0.
SMRiQ5L3D0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L3D0.

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
HOGENOMiHOG000229091.
KOiK01933.
OMAiLNRHDTV.
OrthoDBiPOG091H0128.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5L3D0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAYKQAGV DIEAGYQAVA LMKEHVQKTM RPEVLGGIGG FGGLFDLSAL
60 70 80 90 100
GYRQPVLISG TDGVGTKLKL AFLLDRHDTI GIDCVAMCVN DIIVQGAEPL
110 120 130 140 150
FFLDYIACGK AVPEKIAAIV KGVADGCVEA GCALIGGETA EMPGMYDEDE
160 170 180 190 200
YDLAGFAVGV AEKERLITGE TIQAGDALVG LPSSGLHSNG YSLVRRIVFE
210 220 230 240 250
QAKLSLDEIY EPLDVPLGEE LLKPTRIYAK LLRSVRERFT IKGMAHITGG
260 270 280 290 300
GLIENIPRML PPGIGARIQL GSWPILPIFD FLREKGSLEE EEMFSVFNMG
310 320 330 340
IGLVLAVSPE TAAPLVEWLS ERGEPAYIIG EVAKGAGVSF AGGGRA
Length:346
Mass (Da):36,816
Last modified:February 1, 2005 - v1
Checksum:i65B292536EB228B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74550.1.
RefSeqiWP_011229774.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD74550; BAD74550; GK0265.
GeneIDi3183396.
KEGGigka:GK0265.
PATRICi21961674. VBIGeoKau81518_0333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD74550.1.
RefSeqiWP_011229774.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Z01X-ray2.20A1-346[»]
ProteinModelPortaliQ5L3D0.
SMRiQ5L3D0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK0265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD74550; BAD74550; GK0265.
GeneIDi3183396.
KEGGigka:GK0265.
PATRICi21961674. VBIGeoKau81518_0333.

Phylogenomic databases

eggNOGiENOG4105CXB. Bacteria.
COG0150. LUCA.
HOGENOMiHOG000229091.
KOiK01933.
OMAiLNRHDTV.
OrthoDBiPOG091H0128.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.

Miscellaneous databases

EvolutionaryTraceiQ5L3D0.

Family and domain databases

CDDicd02196. PurM. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR004733. PurM_cligase.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUR5_GEOKA
AccessioniPrimary (citable) accession number: Q5L3D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.