Q5L3D0 (PUR5_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	GK0265

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus kaustophilus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 346

346

Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741

PRO_0000258356

Secondary structure

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346

Helix Strand Turn

Details...

Helix

18 – 29

12

Helix

47 – 50

4

Beta strand

53 – 63

11

Helix

67 – 74

8

Helix

80 – 93

14

Turn

94 – 96

3

Beta strand

98 – 110

13

Helix

113 – 130

18

Beta strand

133 – 136

4

Beta strand

151 – 162

12

Helix

163 – 165

3

Beta strand

177 – 182

6

Beta strand

184 – 186

3

Helix

191 – 199

9

Helix

217 – 222

6

Helix

229 – 238

10

Beta strand

243 – 246

4

Helix

251 – 259

9

Beta strand

264 – 269

6

Helix

277 – 285

9

Helix

290 – 296

7

Beta strand

301 – 307

7

Helix

309 – 311

3

Helix

312 – 321

10

Beta strand

327 – 341

15

Sequences

	Sequence		Length	Mass (Da)
	Q5L3D0 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 65B292536EB228B9	FASTA	346	36,816
	10 20 30 40 50 60 MAKAYKQAGV DIEAGYQAVA LMKEHVQKTM RPEVLGGIGG FGGLFDLSAL GYRQPVLISG 70 80 90 100 110 120 TDGVGTKLKL AFLLDRHDTI GIDCVAMCVN DIIVQGAEPL FFLDYIACGK AVPEKIAAIV 130 140 150 160 170 180 KGVADGCVEA GCALIGGETA EMPGMYDEDE YDLAGFAVGV AEKERLITGE TIQAGDALVG 190 200 210 220 230 240 LPSSGLHSNG YSLVRRIVFE QAKLSLDEIY EPLDVPLGEE LLKPTRIYAK LLRSVRERFT 250 260 270 280 290 300 IKGMAHITGG GLIENIPRML PPGIGARIQL GSWPILPIFD FLREKGSLEE EEMFSVFNMG 310 320 330 340 IGLVLAVSPE TAAPLVEWLS ERGEPAYIIG EVAKGAGVSF AGGGRA

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000043 Genomic DNA. Translation: BAD74550.1.

RefSeq

YP_146118.1. NC_006510.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z01	X-ray	2.20	A	1-346	[»]

ProteinModelPortal

Q5L3D0.

SMR

Q5L3D0. Positions 17-342.

ModBase

Search...

Protein-protein interaction databases

STRING

235909.GK0265.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD74550; BAD74550; GK0265.

GeneID

3183396.

KEGG

gka:GK0265.

PATRIC

21961674. VBIGeoKau81518_0333.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0150.

HOGENOM

HOG000229091.

KO

K01933.

OMA

IDMIAMN.

ProtClustDB

PRK05385.

Enzyme and pathway databases

BioCyc

GKAU235909:GJO7-316-MONOMER.

UniPathway

UPA00074; UER00129.

Family and domain databases

HAMAP

MF_00741_B. AIRS_B.

InterPro

IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]

Pfam

PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]

SUPFAM

SSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.

TIGRFAMs

TIGR00878. purM. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q5L3D0.

Entry information

Entry name

PUR5_GEOKA

Accession

Primary (citable) accession number: Q5L3D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program