Q5L2X9 (CYSH_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	GK0416

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008926

Sequences

	Sequence		Length	Mass (Da)
	Q5L2X9 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: F5E7E76B30CE3C44	FASTA	235	27,144
	10 20 30 40 50 60 MLTYDQWEKA EKPSFPSDNE TKGALDVLAW AYREYGDEIV YACSFGIEGI VLIDLISQVK 70 80 90 100 110 120 PDAEIVFLDT GLHFPETYDT IAKVKEKYPS LRIVMKQPRL TLEEQKAQFG DELWKRDPNK 130 140 150 160 170 180 CCELRKVIPL REVLTGVTAW ISGLRREQSP TRRHVEYINK DDKFRSIKVC PLIHWTWKDV 190 200 210 220 230 WNYVYKRHLP YNVLHDRGYP SIGCAPCTAP ATDPNDLRSG RWAGQGKTEC GLHLA

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD74701.1.
RefSeq	YP_146269.1. NC_006510.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
ProteinModelPortal	Q5L2X9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3184788.
GenomeReviews	Gene locus GK0416 in contig BA000043_GR.
KEGG	gka:GK0416.
NMPDR	fig|235909.3.peg.1907.
PATRIC	21961990. VBIGeoKau81518_0491.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG758022.
OMA	AIHGTRF.
ProtClustDB	CLSK2766836.
Enzyme and pathway databases
BioCyc	GKAU235909:GK0416-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. CysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_GEOKA

Accession

Primary (citable) accession number: Q5L2X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program