Reviewed, UniProtKB/Swiss-Prot Q5L1V4 (ARGJ_GEOKA)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	GK0791

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity.
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm Probable.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000227228
	Chain	197 – 410	214	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000227229
Sites
	Site	196 – 197	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L1V4-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: DF461D99F0376E69	FASTA	410	43,476
	10 20 30 40 50 60 MTATKQTAQV TAVADGTVVT PKGFQAAGVH AGLRYSKKDL GVILCDVPAS AAAVYTQSHF 70 80 90 100 110 120 QAAPLKVTQA SLAVEQKLQA VIVNSACANA CTGEQGLKDA YEMRELCAKQ FGLALHHVAV 130 140 150 160 170 180 ASTGVIGEYL PMEKIRAGIK QLVPGVTMAD AEAFQTAILT TDTVMKRACY QTTVDGKTVT 190 200 210 220 230 240 VGGAAKGSGM IHPNMATMLA FITTDANISS AVLHDALRSI TDVSFNQITV DGDTSTNDMV 250 260 270 280 290 300 VVMASGLAGN DELTPNHPDW EHFYEALRKT CEDLAKQIAR DGEGATKLIE VRVRGAKTDE 310 320 330 340 350 360 EAKKIAKQIV GSNLVKTAVY GADANWGRII GAIGYSDAEV NPDNVDVAIG PIVMLKGSEP 370 380 390 400 410 QPFSEEEATA YLQQETVVIE VDLHLGDGFG VAWGCDLTYD YVKINASYRT

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD75076.1.
RefSeq	YP_146644.1.
3D structure databases
SMR	Q5L1V4. Positions 197-408.
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	3185135.
GenomeReviews	Gene locus GK0791 in contig BA000043_GR.
KEGG	gka:GK0791.
NMPDR	fig|235909.3.peg.1442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L1V4.
OMA	Q5L1V4. IVNSGNA.
Enzyme and pathway databases
BioCyc	GKAU235909:GK0791-MON.
BRENDA	2.3.1.1. 281547. 2.3.1.35. 281547.
Family and domain databases
HAMAP	MF_01106. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. ArgJ. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_GEOKA

Accession

Primary (citable) accession number: Q5L1V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)