Q5L1R3 (PRPE_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
EC=3.6.1.17

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	GK0832

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: EC nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297703

Sequences

	Sequence		Length	Mass (Da)
	Q5L1R3 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5512991682FA220F	FASTA	245	27,839
	10 20 30 40 50 60 MQVDIIGDIH GCFREFAALT ETLGYHWDEG VPIHPDGRKL GFVGDLTDRG PESLNMIEIV 70 80 90 100 110 120 CALVERNLAY YVPGNHCNKL YRFFLGRNVQ IAHGLETTVA EYRTLPPSEQ EMIRRKFIRL 130 140 150 160 170 180 YEQAPLYAVL DEGRLVIAHA GIRHDYIGRT DKKVKTFVLY GDITGEMNQD GTPVRRDWAK 190 200 210 220 230 240 RYHGSAWVVY GHTPVEKPRF VGRTVNIDTG CVFGGALTAL RYPEMETVSV PSSMPFVPEK FRTFS

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD75117.1.
RefSeq	YP_146685.1. NC_006510.1.
3D structure databases
ProteinModelPortal	Q5L1R3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3185400.
GenomeReviews	Gene locus GK0832 in contig BA000043_GR.
KEGG	gka:GK0832.
NMPDR	fig|235909.3.peg.1522.
PATRIC	21962890. VBIGeoKau81518_0916.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724457.
OMA	YVPGNHC.
ProtClustDB	PRK13625.
Enzyme and pathway databases
BioCyc	GKAU235909:GK0832-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR023937. Bis(5'-nucleosyl)-tetraP_PrpE. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01090.
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_GEOKA

Accession

Primary (citable) accession number: Q5L1R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program