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Protein

2,3-diketo-5-methylthiopentyl-1-phosphate enolase

Gene

mtnW

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P).1 Publication

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 3 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (mtnB)
  3. 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)
  4. 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX)
  5. Acireductone dioxygenase (mtnD)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Proton acceptor
Binding sitei147 – 1471Substrate
Metal bindingi173 – 1731Magnesium; via carbamate group
Metal bindingi175 – 1751Magnesium
Metal bindingi176 – 1761Magnesium
Binding sitei264 – 2641Substrate
Binding sitei337 – 3371Substrate; via amide nitrogen

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1028-MONOMER.
BRENDAi5.3.2.5. 8138.
UniPathwayiUPA00904; UER00876.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-diketo-5-methylthiopentyl-1-phosphate enolase (EC:5.3.2.5)
Short name:
DK-MTP-1-P enolase
Alternative name(s):
RuBisCO-like protein
Short name:
RLP
Gene namesi
Name:mtnW
Ordered Locus Names:GK0953
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi98 – 981K → A: Loss of enolase activity. 1 Publication
Mutagenesisi147 – 1471K → A: Same activity as the wild-type. 1 Publication
Mutagenesisi173 – 1731K → A: Same activity as the wild-type. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4134132,3-diketo-5-methylthiopentyl-1-phosphate enolasePRO_0000062694Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731N6-carboxylysine1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi235909.GK0953.

Structurei

Secondary structure

1
413
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1412Combined sources
Helixi16 – 2611Combined sources
Turni27 – 293Combined sources
Helixi37 – 437Combined sources
Helixi44 – 463Combined sources
Beta strandi49 – 557Combined sources
Helixi60 – 667Combined sources
Beta strandi71 – 8010Combined sources
Helixi81 – 833Combined sources
Helixi88 – 969Combined sources
Helixi98 – 1014Combined sources
Beta strandi102 – 11110Combined sources
Helixi114 – 1174Combined sources
Helixi126 – 1349Combined sources
Beta strandi141 – 1455Combined sources
Helixi154 – 16613Combined sources
Beta strandi170 – 1734Combined sources
Beta strandi182 – 1843Combined sources
Helixi186 – 20419Combined sources
Beta strandi209 – 2135Combined sources
Helixi218 – 2203Combined sources
Helixi221 – 23010Combined sources
Beta strandi234 – 2385Combined sources
Helixi240 – 2423Combined sources
Helixi245 – 2539Combined sources
Turni255 – 2573Combined sources
Beta strandi261 – 2633Combined sources
Helixi268 – 2703Combined sources
Beta strandi275 – 2795Combined sources
Helixi281 – 2844Combined sources
Helixi287 – 2926Combined sources
Beta strandi295 – 3006Combined sources
Beta strandi302 – 3065Combined sources
Helixi310 – 32112Combined sources
Beta strandi325 – 3273Combined sources
Beta strandi331 – 3377Combined sources
Helixi340 – 3423Combined sources
Helixi343 – 3508Combined sources
Beta strandi352 – 3598Combined sources
Helixi360 – 3634Combined sources
Helixi368 – 38518Combined sources
Helixi389 – 3935Combined sources
Helixi397 – 40610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OEJX-ray2.55A/B1-413[»]
2OEKX-ray1.80A/B1-413[»]
2OELX-ray1.80A/B1-413[»]
2OEMX-ray1.70A/B1-413[»]
ProteinModelPortaliQ5L1E2.
SMRiQ5L1E2. Positions 2-413.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L1E2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1764Substrate binding
Regioni359 – 3602Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DT1. Bacteria.
COG1850. LUCA.
HOGENOMiHOG000230832.
KOiK08965.
OMAiKDDEIFF.
OrthoDBiPOG091H0DKL.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01679. Salvage_MtnW. 1 hit.
InterProiIPR017717. Diketo-Methiopentyl-P_enolase.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03332. salvage_mtnW. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5L1E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVMATYLL HDETDIRKKA EGIALGLTIG TWTDLPALEQ EQLRKHKGEV
60 70 80 90 100
VAIEELGESE RVNAYFGKRL KRAIVKIAYP TVNFSADLPA LLVTTFGKLS
110 120 130 140 150
LDGEVRLLDL EFPDEWKRQF PGPRFGIDGI RDRVGVHNRP LLMSIFKGMI
160 170 180 190 200
GRDLAYLTSE LKKQALGGVD LVKDDEILFD SELLPFEKRI TEGKAALQEV
210 220 230 240 250
YEQTGKRTLY AVNLTGKTFA LKDKAKRAAE LGADVLLFNV FAYGLDVLQA
260 270 280 290 300
LREDEEIAVP IMAHPAFSGA VTPSEFYGVA PSLWLGKLLR LAGADFVLFP
310 320 330 340 350
SPYGSVALER EQALGIARAL TDDQEPFARA FPVPSAGIHP GLVPLIIRDF
360 370 380 390 400
GLDTIVNAGG GIHGHPDGAI GGGRAFRAAI DAVLAGRPLR AAAAENEALQ
410
KAIDRWGVVE VEA
Length:413
Mass (Da):44,906
Last modified:February 1, 2005 - v1
Checksum:i560DBEA2E17E0310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75238.1.
RefSeqiWP_011230454.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD75238; BAD75238; GK0953.
GeneIDi3185264.
KEGGigka:GK0953.
PATRICi21963143. VBIGeoKau81518_1041.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75238.1.
RefSeqiWP_011230454.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OEJX-ray2.55A/B1-413[»]
2OEKX-ray1.80A/B1-413[»]
2OELX-ray1.80A/B1-413[»]
2OEMX-ray1.70A/B1-413[»]
ProteinModelPortaliQ5L1E2.
SMRiQ5L1E2. Positions 2-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK0953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD75238; BAD75238; GK0953.
GeneIDi3185264.
KEGGigka:GK0953.
PATRICi21963143. VBIGeoKau81518_1041.

Phylogenomic databases

eggNOGiENOG4105DT1. Bacteria.
COG1850. LUCA.
HOGENOMiHOG000230832.
KOiK08965.
OMAiKDDEIFF.
OrthoDBiPOG091H0DKL.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00876.
BioCyciGKAU235909:GJO7-1028-MONOMER.
BRENDAi5.3.2.5. 8138.

Miscellaneous databases

EvolutionaryTraceiQ5L1E2.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01679. Salvage_MtnW. 1 hit.
InterProiIPR017717. Diketo-Methiopentyl-P_enolase.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03332. salvage_mtnW. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTNW_GEOKA
AccessioniPrimary (citable) accession number: Q5L1E2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Has no RuBP-carboxylation activity.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.