Reviewed, UniProtKB/Swiss-Prot Q5L172 (ODO1_GEOKA)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	odhA
Ordered Locus Names:	GK1023

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	950 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity. HAMAP MF_01169
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2. HAMAP MF_01169
Cofactor	Thiamine pyrophosphate By similarity. HAMAP MF_01169
Subunit structure	Homodimer By similarity. HAMAP MF_01169
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 950	950	2-oxoglutarate dehydrogenase E1 component HAMAP MF_01169		PRO_0000162172

Sequences

	Sequence		Length	Mass (Da)
	Q5L172-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 0A9769B6BB8BC10F	FASTA	950	107,561
	10 20 30 40 50 60 MAKQTNYAQP WSQFYGPNLG YVIEMYEQYL DDPNSIDPEL KQLFEQWGAP VLEEPVSPAD 70 80 90 100 110 120 DETAKTHQTF RLPETPTIFS KLVAAVKLAD SIRHYGHLVA DTNPLVKKEK KLRRLELDEY 130 140 150 160 170 180 DLTEEDLKRI PVAFLCPHAP AHVKNGWDAI LHLRKIYTDK IAFEFSQVHN LEERNWLIQQ 190 200 210 220 230 240 IESGAYYPSL ANKERVALLR RLTEVEGFEK FIHRTYVGQK RFSIEGLDSM VPLLDELVRQ 250 260 270 280 290 300 AIEHEIDAVN IGMAHRGRLN VLAHVLGKPY EMIFAEFQHA ESKNFIPSEG SVAITYGWTG 310 320 330 340 350 360 DVKYHLGAAR RLRNQSAHTM RITLANNPSH LEVVNPVVLG YTRAAQEDRT KPGVPVQNTD 370 380 390 400 410 420 ASFAILIHGD AAFPGQGIVA ETLNLSQLRG YTTGGTIHII ANNMIGFTTE SYDSRSTTYA 430 440 450 460 470 480 SDMAKGFEVP IVHVNADDPE ACLAAACLAF AYRQRFKKDF VIDLIGYRRF GHNEMDEPMA 490 500 510 520 530 540 TNPTMYAIIN QHPTVRKLYA QKLMEKGIIT EREVDEMEQE VAERLKIAYE RVPKNEDELD 550 560 570 580 590 600 FIMDPPKPVV DRLPEVKTSV AKDVLHRVNE ELLQFPDGFN VFNKLERILK RRSGVFAQNG 610 620 630 640 650 660 KVDWAHAEIL AFATILQDGV PIRLTGQDSQ RGTFAQRHLV LHDVKTGEEY VPLHHISGAK 670 680 690 700 710 720 ASFVVYNSPL TEAAVLGYEY GYNVYAPETL VLWEAQFGDF ANMAQVMFDQ FISSGRAKWG 730 740 750 760 770 780 QKSGLVMLLP HGYEGQGPEH SSGRVERFLQ LAAENNWTVA NLSTAAQYFH ILRRQAALLT 790 800 810 820 830 840 REEVRPLIIM TPKSLLRHPL AASDAEVFVD GAFSPVLEQP GLGADAGKVE RIVFGTGKLM 850 860 870 880 890 900 IDLAEQIGKM ESLDWLHIVR IEELYPFPEE AVKDIIARYP NVKELVWVQE EPKNMGAWLY 910 920 930 940 950 MEPRLRALAP EGVDVSYIGR RRRASPAEGD PVVHRKEQER IIRCALTKHE

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD75308.1.
RefSeq	YP_146876.1.
3D structure databases
SMR	Q5L172. Positions 81-947.
ModBase	Search...
Genome annotation databases
GeneID	3184920.
GenomeReviews	Gene locus GK1023 in contig BA000043_GR.
KEGG	gka:GK1023.
NMPDR	fig|235909.3.peg.1939.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289950.
OMA	EGDEPAF.
Enzyme and pathway databases
BioCyc	GKAU235909:GK1023-MONOMER.
BRENDA	1.2.4.2. 281547.
Family and domain databases
HAMAP	MF_01169. SucA_OdhA. [Tree]
InterPro	IPR011603. 2oxoglutarate_DH_E1. IPR001017. DH_E1. IPR005475. Transketolase-like_Pyr-bd. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_GEOKA

Accession

Primary (citable) accession number: Q5L172

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)