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Q5L0V9

- SYI_GEOKA

UniProt

Q5L0V9 - SYI_GEOKA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:GK1136
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Isoleucine--tRNA ligasePRO_0000098390Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK1136.

Structurei

3D structure databases

ProteinModelPortaliQ5L0V9.
SMRiQ5L0V9. Positions 2-871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L0V9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKETLLMP KTDFPMRGNL PKREPEMQKK WEEMDIYRKV QERTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KILKDIIVRY KSMSGYCAPY VPGWDTHGLP
110 120 130 140 150
IETALAKQGV DRKSMSVAEF RKRCEQYAYE QIDNQRRQFK RLGVRGDWDN
160 170 180 190 200
PYITLKPEYE AQQIKVFGEM AKKGLIYKGL KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
KDKRSPSIYV AFPVKNGKGV LEGDERIVIW TTTPWTIPAN LAIAVHPDLD
260 270 280 290 300
YHVVDVSGKR YVVAAALAES VAKEIGWDAW SVVKTVKGKE LEYVVARHPF
310 320 330 340 350
YERDSLVVCG EHVTTDAGTG CVHTAPGHGE DDFLVGQKYG LPVLCPVDER
360 370 380 390 400
GYMTEEAPGF AGMFYEDANK AITQKLEEVG ALLKLGFITH SYPHDWRTKQ
410 420 430 440 450
PTIFRATTQW FASIDKIRNE LLQAIKETKW IPEWGEIRIH NMVRDRGDWC
460 470 480 490 500
ISRQRAWGVP IPVFYGENGE PIITDETIEH VSNLFRQYGS NVWFEREAKD
510 520 530 540 550
LLPEGFTHPS SPNGIFTKET DIMDVWFDSG SSHQAVLVER DDLVRPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSLGN
610 620 630 640 650
VVVPAKVMEQ FGADILRLWV ASVDYQADVR ISDHILKQVS EVYRKIRNTF
660 670 680 690 700
RFMLGNLFDF DPNQNAVPIG ELGEVDRYML AKLNKLIAKV KKAYDSYDFA
710 720 730 740 750
AVYHEMNHFC TVELSAFYLD MAKDILYIEA ADSRARRAVQ TVLYETVVAL
760 770 780 790 800
AKLIAPILPH TADEVWEHIP NRRENVESVQ LTDMPEPIAI DGEEALLAKW
810 820 830 840 850
DAFMDVRDDI FKALENARNE KVIGKSLTAS VIVYPKDEAR KLLASLDADL
860 870 880 890 900
RQLLIVSAFS IADEPYDAAP AEAERLDHVA VIVRPAEGET CERCWTVTKE
910 920
VGADPSHPTL CPRCAHIVNE HYSA
Length:924
Mass (Da):104,696
Last modified:February 1, 2005 - v1
Checksum:i887AB7BF2479F713
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000043 Genomic DNA. Translation: BAD75421.1.
RefSeqiWP_011230636.1. NC_006510.1.
YP_146989.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD75421; BAD75421; GK1136.
GeneIDi3184639.
KEGGigka:GK1136.
PATRICi21963537. VBIGeoKau81518_1238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000043 Genomic DNA. Translation: BAD75421.1 .
RefSeqi WP_011230636.1. NC_006510.1.
YP_146989.1. NC_006510.1.

3D structure databases

ProteinModelPortali Q5L0V9.
SMRi Q5L0V9. Positions 2-871.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 235909.GK1136.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD75421 ; BAD75421 ; GK1136 .
GeneIDi 3184639.
KEGGi gka:GK1136.
PATRICi 21963537. VBIGeoKau81518_1238.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci GKAU235909:GJO7-1211-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiSYI_GEOKA
AccessioniPrimary (citable) accession number: Q5L0V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3