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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:GK1136
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Isoleucine--tRNA ligasePRO_0000098390Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK1136.

Structurei

3D structure databases

ProteinModelPortaliQ5L0V9.
SMRiQ5L0V9. Positions 2-871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L0V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLMP KTDFPMRGNL PKREPEMQKK WEEMDIYRKV QERTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KILKDIIVRY KSMSGYCAPY VPGWDTHGLP
110 120 130 140 150
IETALAKQGV DRKSMSVAEF RKRCEQYAYE QIDNQRRQFK RLGVRGDWDN
160 170 180 190 200
PYITLKPEYE AQQIKVFGEM AKKGLIYKGL KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
KDKRSPSIYV AFPVKNGKGV LEGDERIVIW TTTPWTIPAN LAIAVHPDLD
260 270 280 290 300
YHVVDVSGKR YVVAAALAES VAKEIGWDAW SVVKTVKGKE LEYVVARHPF
310 320 330 340 350
YERDSLVVCG EHVTTDAGTG CVHTAPGHGE DDFLVGQKYG LPVLCPVDER
360 370 380 390 400
GYMTEEAPGF AGMFYEDANK AITQKLEEVG ALLKLGFITH SYPHDWRTKQ
410 420 430 440 450
PTIFRATTQW FASIDKIRNE LLQAIKETKW IPEWGEIRIH NMVRDRGDWC
460 470 480 490 500
ISRQRAWGVP IPVFYGENGE PIITDETIEH VSNLFRQYGS NVWFEREAKD
510 520 530 540 550
LLPEGFTHPS SPNGIFTKET DIMDVWFDSG SSHQAVLVER DDLVRPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSLGN
610 620 630 640 650
VVVPAKVMEQ FGADILRLWV ASVDYQADVR ISDHILKQVS EVYRKIRNTF
660 670 680 690 700
RFMLGNLFDF DPNQNAVPIG ELGEVDRYML AKLNKLIAKV KKAYDSYDFA
710 720 730 740 750
AVYHEMNHFC TVELSAFYLD MAKDILYIEA ADSRARRAVQ TVLYETVVAL
760 770 780 790 800
AKLIAPILPH TADEVWEHIP NRRENVESVQ LTDMPEPIAI DGEEALLAKW
810 820 830 840 850
DAFMDVRDDI FKALENARNE KVIGKSLTAS VIVYPKDEAR KLLASLDADL
860 870 880 890 900
RQLLIVSAFS IADEPYDAAP AEAERLDHVA VIVRPAEGET CERCWTVTKE
910 920
VGADPSHPTL CPRCAHIVNE HYSA
Length:924
Mass (Da):104,696
Last modified:February 1, 2005 - v1
Checksum:i887AB7BF2479F713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75421.1.
RefSeqiWP_011230636.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD75421; BAD75421; GK1136.
GeneIDi3184639.
KEGGigka:GK1136.
PATRICi21963537. VBIGeoKau81518_1238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75421.1.
RefSeqiWP_011230636.1. NC_006510.1.

3D structure databases

ProteinModelPortaliQ5L0V9.
SMRiQ5L0V9. Positions 2-871.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK1136.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD75421; BAD75421; GK1136.
GeneIDi3184639.
KEGGigka:GK1136.
PATRICi21963537. VBIGeoKau81518_1238.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1211-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiSYI_GEOKA
AccessioniPrimary (citable) accession number: Q5L0V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.