Reviewed, UniProtKB/Swiss-Prot Q5L0U5 (PYRC_GEOKA)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	GK1150

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Dihydroorotase		PRO_1000024083
Sites
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1; via carbamate group By similarity
	Metal binding	143	1	Zinc 2; via carbamate group By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	233	1	Zinc 2 By similarity
	Metal binding	306	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	143	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L0U5-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 51B1DC25EE06C01A	FASTA	428	46,177
	10 20 30 40 50 60 MGVWLKNGMS FNKDGELMRT HIKIEHGTIA AILYEQPLEA NEEDVIDVGG RLIVPGLIDL 70 80 90 100 110 120 HVHLREPGGE AKETIETGTL AAAKGGFTTV AAMPNTNPAP DRKEQMEWLQ ARIRETARVN 130 140 150 160 170 180 VLPYAAITIG QKGEELTDFA ALKEAGAFAF TDDGVGVQSA GMMFEAMKQA AALDMAIVAH 190 200 210 220 230 240 CEDDTLTNGG AVHDGEFARR YGLRGIPSVC EAVHIARDVL LAEAAGCHYH VCHISTKESV 250 260 270 280 290 300 RVVRDAKRAG IRVTAEVTPH HLLLCDEDIP GLDANYKMNP PLRSREDRDA LIEGLLDGTI 310 320 330 340 350 360 DFIATDHAPH TAAEKAKGIE AAPFGIVGLE TAFPLLYTHF VKTGVFTLKQ LVDWLTIKPA 370 380 390 400 410 420 QCFGLKAGRL AVGAPADIAV IDLETEEAID PETFASKGKN TPFAGWVCQG WPVMTFVGGT LVWEKGRA

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD75435.1.
RefSeq	YP_147003.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3185137.
GenomeReviews	Gene locus GK1150 in contig BA000043_GR.
KEGG	gka:GK1150.
NMPDR	fig|235909.3.peg.2213.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L0U5.
Enzyme and pathway databases
BioCyc	GKAU235909:GK1150-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_GEOKA

Accession

Primary (citable) accession number: Q5L0U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 2005
Last modified:	September 2, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)