Q5L0N4 (Q5L0N4_GEOKA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAD75496.1
Ordered Locus Names:	GK1211

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5L0N4 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: E54E091043AC5300	FASTA	691	79,131
	10 20 30 40 50 60 MSDYLVIVES PTKAKTIERY LGKKYTVKAS MGHVRDLPKS QMGVDIDHGY EPKYITIRGK 70 80 90 100 110 120 GQVIKELKTA AKKAKKVFLA ADPDREGEAI AWHLAHMLDL DIHSDCRVVF HEITKDAIQQ 130 140 150 160 170 180 SFQQPRAINM NLVDAQQARR VLDRLVGYNI SPLLWKKVKK GLSAGRVQSV ALRLIIDRER 190 200 210 220 230 240 EIREFQPEEY WTIQATFQKE GETFAASFYS IDGQKRDLKT EADVKAVLDR LNGTAFAVKT 250 260 270 280 290 300 VTKRERKRSP VPPFTTSSLQ QEAARKLNFR TKKTMMIAQQ LYEGIDLGSE GTVGLITYMR 310 320 330 340 350 360 TDSTRVAETA QQEAAAYIEA TFGAMYVNQE KRKEKKSTNA QDAHEAIRPT SAFRDPDKVK 370 380 390 400 410 420 PYLTRDQFRL YKLIWERFIA SQMAAAVLDT MSVELENNGV VFRASGSKVK FPGFMKVYIE 430 440 450 460 470 480 GTDDQTEEQD RILPDLEEEE TVESETIESK QHFTQPPPRY TEARLVKTLE ELGIGRPSTY 490 500 510 520 530 540 APTLDTIQKR NYVVLENKRF VPTELGEIVV ELMLEFFPEI IDVEFTAKME KELDEIEEGK 550 560 570 580 590 600 VEWIKVVDEF YREFEKRLKV AEKEMRAVEI KDEPAGIDCE VCGSPMVYKM GRFGKFIACS 610 620 630 640 650 660 NFPECRHTKP IVKEIGVKCP KCREGNIVER STKRKRVFYG CDRFPDCDFV SWDKPLARPC 670 680 690 PKCAGLLVEK VLKKGVQVQC TACDYEERLE A

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD75496.1.
RefSeq	YP_147064.1. NC_006510.1.
3D structure databases
ProteinModelPortal	Q5L0N4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3184003.
GenomeReviews	Gene locus GK1211 in contig BA000043_GR.
KEGG	gka:GK1211.
NMPDR	fig|235909.3.peg.2429.
PATRIC	21963684. VBIGeoKau81518_1311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK05582.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q5L0N4_GEOKA

Accession

Primary (citable) accession number: Q5L0N4

Entry history

Integrated into UniProtKB/TrEMBL:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)