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Protein

DNA polymerase III PolC-type

Gene

polC

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity.UniRule annotationSAAS annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi346 – 3461Magnesium 1; via tele nitrogenCombined sources
Metal bindingi346 – 3461Manganese 1; via tele nitrogenCombined sources
Metal bindingi348 – 3481Magnesium 1; via tele nitrogenCombined sources
Metal bindingi348 – 3481Manganese 1; via tele nitrogenCombined sources
Metal bindingi355 – 3551Zinc 1Combined sources
Metal bindingi380 – 3801Zinc 1; via tele nitrogenCombined sources
Metal bindingi405 – 4051Magnesium 1Combined sources
Metal bindingi405 – 4051Magnesium 2Combined sources
Metal bindingi405 – 4051Manganese 1Combined sources
Metal bindingi405 – 4051Manganese 2Combined sources
Metal bindingi620 – 6201Magnesium 2; via pros nitrogenCombined sources
Metal bindingi620 – 6201Manganese 2; via pros nitrogenCombined sources
Metal bindingi670 – 6701Magnesium 2Combined sources
Metal bindingi670 – 6701Manganese 2Combined sources
Metal bindingi743 – 7431Magnesium 1Combined sources
Metal bindingi743 – 7431Manganese 1Combined sources
Metal bindingi745 – 7451Zinc 1; via tele nitrogenCombined sources
Metal bindingi919 – 9191Zinc 2Combined sources
Metal bindingi922 – 9221Zinc 2Combined sources
Metal bindingi944 – 9441Zinc 2Combined sources
Metal bindingi947 – 9471Zinc 2Combined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymeraseUniRule annotationSAAS annotationImported, ExonucleaseUniRule annotationSAAS annotation, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replicationUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumCombined sources, ManganeseCombined sources, Metal-bindingCombined sources, ZincCombined sources

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III PolC-typeUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
PolIIIUniRule annotation
Gene namesi
Name:polCUniRule annotationImported
Ordered Locus Names:GK1258Imported
OrganismiGeobacillus kaustophilus (strain HTA426)Imported
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi235909.GK1258.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F2BX-ray2.39A228-424[»]
A618-1444[»]
3F2CX-ray2.50A228-424[»]
A618-1444[»]
3F2DX-ray2.51A228-424[»]
A618-1444[»]
ProteinModelPortaliQ5L0J3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L0J3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini343 – 41068POLIIIAcInterPro annotationAdd
BLAST
Domaini428 – 594167ExonucleaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-C family. PolC subfamily.UniRule annotationSAAS annotation
Contains exonuclease domain.SAAS annotation

Phylogenomic databases

eggNOGiENOG4105DE5. Bacteria.
COG2176. LUCA.
HOGENOMiHOG000058064.
KOiK03763.
OMAiQGCTGVK.
OrthoDBiEOG6ZWJJS.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00356. DNApol_PolC.
InterProiIPR011708. DNA_pol3_alpha.
IPR024754. DNA_polC-like_N_II.
IPR028112. DNA_polC-type_N_I.
IPR029460. DNAPol_HHH.
IPR006054. DnaQ.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012340. NA-bd_OB-fold.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR006308. PolC_gram_pos.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF14480. DNA_pol3_a_NI. 1 hit.
PF11490. DNA_pol3_a_NII. 1 hit.
PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00573. dnaq. 1 hit.
TIGR01405. polC_Gram_pos. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5L0J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLRGEQTDV MVTKEQKERF LILLEQLKMT SDEWMPHFRE AAIRKVVIDK
60 70 80 90 100
EEKSWHFYFQ FDNVLPVHVY KTFADRLQTA FRHIAAVRHT MEVEAPRVTE
110 120 130 140 150
ADVQAYWPLC LAELQEGMSP LVDWLSRQTP ELKGNKLLVV ARHEAEALAI
160 170 180 190 200
KRRFAKKIAD VYASFGFPPL QLDVSVEPSK QEMEQFLAQK QQEDEERALA
210 220 230 240 250
VLTDLAREEE KAASAPPSGP LVIGYPIRDE EPVRRLETIV EEERRVVVQG
260 270 280 290 300
YVFDAEVSEL KSGRTLLTMK ITDYTNSILV KMFSRDKEDA ELMSGVKKGM
310 320 330 340 350
WVKVRGSVQN DTFVRDLVII ANDLNEIAAN ERQDTAPEGE KRVELHLHTP
360 370 380 390 400
MSQMDAVTSV TKLIEQAKKW GHPAIAVTDH AVVQSFPEAY SAAKKHGMKV
410 420 430 440 450
IYGLEANIVD DGVPIAYNET HRRLSEETYV VFDVETTGLS AVYNTIIELA
460 470 480 490 500
AVKVKDGEII DRFMSFANPG HPLSVTTMEL TGITDEMVKD APKPDEVLAR
510 520 530 540 550
FVDWAGDATL VAHNASFDIG FLNTGLARMG RGKIANPVID TLELARFLYP
560 570 580 590 600
DLKNHRLNTL CKKFDIELTQ HHRAIYDAEA TGHLLMRLLK EAEERGILFH
610 620 630 640 650
DELNSRTHSE ASYRLARPFH VTLLAQNETG LKNLFKLVSL SHIQYFHRVP
660 670 680 690 700
RIPRSVLVKH RDGLLVGSGC DKGELFDNLI QKAPEEVEDI ARFYDFLEVH
710 720 730 740 750
PPDVYKPLIE MDYVKDEEMI KNIIRSIVAL GEKLDIPVVA TGNVHYLNPE
760 770 780 790 800
DKIYRKILIH SQGGANPLNR HELPDVYFRT TNEMLDCFSF LGPEKAKEIV
810 820 830 840 850
VDNTQKIASL IGDVKPIKDE LYTPRIEGAD EEIREMSYRR AKEIYGDPLP
860 870 880 890 900
KLVEERLEKE LKSIIGHGFA VIYLISHKLV KKSLDDGYLV GSRGSVGSSF
910 920 930 940 950
VATMTEITEV NPLPPHYVCP NCKHSEFFND GSVGSGFDLP DKNCPRCGTK
960 970 980 990 1000
YKKDGHDIPF ETFLGFKGDK VPDIDLNFSG EYQPRAHNYT KVLFGEDNVY
1010 1020 1030 1040 1050
RAGTIGTVAD KTAYGFVKAY ASDHNLELRG AEIDRLAAGC TGVKRTTGQH
1060 1070 1080 1090 1100
PGGIIVVPDY MEIYDFTPIQ YPADDTSSEW RTTHFDFHSI HDNLLKLDIL
1110 1120 1130 1140 1150
GHDDPTVIRM LQDLSGIDPK TIPTDDPDVM GIFSSTEPLG VTPEQIMCNV
1160 1170 1180 1190 1200
GTIGIPEFGT RFVRQMLEET RPKTFSELVQ ISGLSHGTDV WLGNAQELIQ
1210 1220 1230 1240 1250
NGTCTLSEVI GCRDDIMVYL IYRGLEPSLA FKIMESVRKG KGLTPEFEAE
1260 1270 1280 1290 1300
MRKHDVPEWY IDSCKKIKYM FPKAHAAAYV LMAVRIAYFK VHHPLLYYAS
1310 1320 1330 1340 1350
YFTVRAEDFD LDAMIKGSAA IRKRIEEINA KGIQATAKEK SLLTVLEVAL
1360 1370 1380 1390 1400
EMCERGFSFK NIDLYRSQAT EFVIDGNSLI PPFNAIPGLG TNVAQAIVRA
1410 1420 1430 1440
REEGEFLSKE DLQQRGKLSK TLLEYLESRG CLDSLPDHNQ LSLF
Length:1,444
Mass (Da):163,115
Last modified:February 1, 2005 - v1
Checksum:i28DD9C7824C5D03D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75543.1.

Genome annotation databases

EnsemblBacteriaiBAD75543; BAD75543; GK1258.
KEGGigka:GK1258.
PATRICi21963778. VBIGeoKau81518_1358.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75543.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3F2BX-ray2.39A228-424[»]
A618-1444[»]
3F2CX-ray2.50A228-424[»]
A618-1444[»]
3F2DX-ray2.51A228-424[»]
A618-1444[»]
ProteinModelPortaliQ5L0J3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK1258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD75543; BAD75543; GK1258.
KEGGigka:GK1258.
PATRICi21963778. VBIGeoKau81518_1358.

Phylogenomic databases

eggNOGiENOG4105DE5. Bacteria.
COG2176. LUCA.
HOGENOMiHOG000058064.
KOiK03763.
OMAiQGCTGVK.
OrthoDBiEOG6ZWJJS.

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1333-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5L0J3.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_00356. DNApol_PolC.
InterProiIPR011708. DNA_pol3_alpha.
IPR024754. DNA_polC-like_N_II.
IPR028112. DNA_polC-type_N_I.
IPR029460. DNAPol_HHH.
IPR006054. DnaQ.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012340. NA-bd_OB-fold.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR006308. PolC_gram_pos.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF14480. DNA_pol3_a_NI. 1 hit.
PF11490. DNA_pol3_a_NII. 1 hit.
PF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
SM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00573. dnaq. 1 hit.
TIGR01405. polC_Gram_pos. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426Imported.
  2. Cited for: X-RAY CRYSTALLOGRAPHY (2.39 ANGSTROMS) OF 228-424 AND 618-1444 IN COMPLEX WITH MAGNESIUM; MANGANESE AND ZINC.

Entry informationi

Entry nameiQ5L0J3_GEOKA
AccessioniPrimary (citable) accession number: Q5L0J3
Entry historyi
Integrated into UniProtKB/TrEMBL: February 1, 2005
Last sequence update: February 1, 2005
Last modified: July 6, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.