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Protein

Urocanate hydratase

Gene

hutU

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O.UniRule annotation

Cofactori

NAD+UniRule annotationNote: Binds 1 NAD+ per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei406UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Names & Taxonomyi

Protein namesi
Recommended name:
Urocanate hydrataseUniRule annotation (EC:4.2.1.49UniRule annotation)
Short name:
UrocanaseUniRule annotation
Alternative name(s):
Imidazolonepropionate hydrolaseUniRule annotation
Gene namesi
Name:hutUUniRule annotation
Ordered Locus Names:GK1367
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000251281 – 551Urocanate hydrataseAdd BLAST551

Interactioni

Protein-protein interaction databases

STRINGi235909.GK1367.

Structurei

Secondary structure

1551
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 19Combined sources4
Helixi20 – 32Combined sources13
Beta strandi54 – 56Combined sources3
Helixi57 – 69Combined sources13
Beta strandi74 – 79Combined sources6
Beta strandi82 – 88Combined sources7
Beta strandi95 – 103Combined sources9
Helixi106 – 108Combined sources3
Helixi138 – 155Combined sources18
Beta strandi164 – 168Combined sources5
Helixi174 – 176Combined sources3
Helixi177 – 183Combined sources7
Beta strandi187 – 193Combined sources7
Helixi195 – 203Combined sources9
Beta strandi208 – 212Combined sources5
Helixi214 – 226Combined sources13
Beta strandi231 – 237Combined sources7
Helixi239 – 248Combined sources10
Beta strandi254 – 256Combined sources3
Turni265 – 268Combined sources4
Helixi276 – 285Combined sources10
Helixi287 – 310Combined sources24
Helixi322 – 328Combined sources7
Helixi334 – 336Combined sources3
Helixi340 – 343Combined sources4
Helixi346 – 350Combined sources5
Beta strandi354 – 360Combined sources7
Helixi365 – 378Combined sources14
Turni379 – 381Combined sources3
Helixi383 – 395Combined sources13
Beta strandi403 – 408Combined sources6
Helixi412 – 425Combined sources14
Beta strandi428 – 431Combined sources4
Beta strandi433 – 437Combined sources5
Helixi464 – 476Combined sources13
Beta strandi479 – 488Combined sources10
Turni489 – 491Combined sources3
Beta strandi492 – 503Combined sources12
Helixi507 – 530Combined sources24
Helixi534 – 543Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X87X-ray2.40A/B1-551[»]
ProteinModelPortaliQ5L084.
SMRiQ5L084.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5L084.

Family & Domainsi

Sequence similaritiesi

Belongs to the urocanase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.
HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.
OrthoDBiPOG091H0CXO.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKRTVSPP AGTERRAKGW IQEAALRMLN NNLHPDVAER PDELIVYGGI
60 70 80 90 100
GKAARNWECY EAIVDTLLRL ENDETLLIQS GKPVAVFRTH PDAPRVLIAN
110 120 130 140 150
SNLVPAWATW DHFHELDKKG LIMYGQMTAG SWIYIGSQGI VQGTYETFAE
160 170 180 190 200
VARQHFGGTL AGTITLTAGL GGMGGAQPLA VTMNGGVCLA IEVDPARIQR
210 220 230 240 250
RIDTNYLDTM TDSLDAALEM AKQAKEEKKA LSIGLVGNAA EVLPRLVEMG
260 270 280 290 300
FVPDVLTDQT SAHDPLNGYI PAGLTLDEAA ELRARDPKQY IARAKQSIAA
310 320 330 340 350
HVRAMLAMQK QGAVTFDYGN NIRQVAKDEG VDDAFSFPGF VPAYIRPLFC
360 370 380 390 400
EGKGPFRWVA LSGDPEDIYK TDEVILREFS DNERLCHWIR MAQKRIKFQG
410 420 430 440 450
LPARICWLGY GERAKFGKII NDMVAKGELK APIVIGRDHL DSGSVASPNR
460 470 480 490 500
ETEGMKDGSD AIADWPILNA LLNAVGGASW VSVHHGGGVG MGYSIHAGMV
510 520 530 540 550
IVADGTKEAE KRLERVLTTD PGLGVVRHAD AGYELAIRTA KEKGIDMPML

K
Length:551
Mass (Da):60,008
Last modified:February 1, 2005 - v1
Checksum:i68E9C8B53BB21E03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75652.1.

Genome annotation databases

EnsemblBacteriaiBAD75652; BAD75652; GK1367.
KEGGigka:GK1367.
PATRICi21964019. VBIGeoKau81518_1478.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75652.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X87X-ray2.40A/B1-551[»]
ProteinModelPortaliQ5L084.
SMRiQ5L084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK1367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD75652; BAD75652; GK1367.
KEGGigka:GK1367.
PATRICi21964019. VBIGeoKau81518_1478.

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.
HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.
OrthoDBiPOG091H0CXO.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Miscellaneous databases

EvolutionaryTraceiQ5L084.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUTU_GEOKA
AccessioniPrimary (citable) accession number: Q5L084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.