Reviewed, UniProtKB/Swiss-Prot Q5L025 (IOLA1_GEOKA)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 1
      Short name=MMSA dehydrogenase 1
      Short name=MMSDH 1
      Short name=MSDH 1
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 1
      Short name=MSA dehydrogenase 1
    EC=1.2.1.18

Gene names

Name:	iolA1
Ordered Locus Names:	GK1426

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Methylmalonate semialdehyde dehydrogenase [acylating] 1 HAMAP MF_01670		PRO_0000352339
Regions
	Nucleotide binding	180 – 184	5	NAD By similarity
Sites
	Active site	288	1	Nucleophile By similarity
	Binding site	387	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L025-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 700701B15A1F3EDF	FASTA	488	53,705
	10 20 30 40 50 60 MSITKPETTV LKNYIGGQWV ASSGTETLEV PNPATGEVLA RVPISTKEDV DQAVQAAKKA 70 80 90 100 110 120 FATWKDVPVP KRARIMFSFH HLLNQHHEEL AELVVQENGK AYKEAYGEIQ RGIECVEFAA 130 140 150 160 170 180 GAPTLLMGES LSNIAEEIDS EMFRYPLGVV AGITPFNFPM MVPLWMFPLA IVCGNTFVLK 190 200 210 220 230 240 PSERTPILAN KLAELFTEAG APPGVLNVVH GAHEVVNALI DHEDIRAISF VGSQPVAKYV 250 260 270 280 290 300 YERTAAQGKR VQALSGAKNH HIVMPDADVE TAVQHVISSA FGSAGQRCMA CSAVVIVGEN 310 320 330 340 350 360 ETFVRRLKQK ADELIIGNGM DPEVLLTPVI RQSHREKVLG YIQKGIEEGA VLLRDGRKEM 370 380 390 400 410 420 DDRPEGNFLG PTIFDYVTPD MTIAKEEIFA PVLSLLRAND LDEALSYIRK SRYGNGATIY 430 440 450 460 470 480 TKDAKAVRKF REEADAGMLG INVGVPATMA FFPFSGWKDS FYGDLHVNGK DGVNFYTRKK MITSRFDF

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD75711.1.
RefSeq	YP_147279.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3183784.
GenomeReviews	Gene locus GK1426 in contig BA000043_GR.
KEGG	gka:GK1426.
NMPDR	fig|235909.3.peg.275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L025.
OMA	Q5L025. ITAEHRD.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA1_GEOKA

Accession

Primary (citable) accession number: Q5L025

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)