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Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei275 – 2751Acetyl-CoAUniRule annotation
Binding sitei312 – 3121Acetyl-CoAUniRule annotation
Active sitei339 – 3391Proton acceptorUniRule annotation
Binding sitei339 – 3391GlyoxylateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311GlyoxylateUniRule annotation
Metal bindingi459 – 4591MagnesiumUniRule annotation
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei630 – 6301Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-1609-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:GK1533
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Malate synthase GPRO_1000056902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK1533.

Structurei

3D structure databases

ProteinModelPortaliQ5KZR8.
SMRiQ5KZR8. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni456 – 4594Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5KZR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEYVQKGNI QVAKVLYDFV NEELLPNSGL DQDKFWSDFG ALISDLTPRN
60 70 80 90 100
KELLARRDEI QEKLNEWHKA HRGRFDFNEY KAFLTDIGYL EPEVEDFEIT
110 120 130 140 150
TDNVDDEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE
160 170 180 190 200
DGAERGSSYN PVRGAKVIAY GRQFLDEAVP LAQYSHKDAV QYAIVDGQLV
210 220 230 240 250
VTTEGGATTG LKEPEKFVGF QGDPQHPTAV LLKNNGLHIE IQIDREHAVG
260 270 280 290 300
KTDQAGVKDI VLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LFGLVKGDLT
310 320 330 340 350
ATFEKNGKIM TRSLNPDRVY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI
360 370 380 390 400
LHANGEEVHE GIMDAVITSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS
410 420 430 440 450
AEVAFANELF DRVEDMLGLE RNTIKIGVMD EERRTSLNLK NCIYQVRDRI
460 470 480 490 500
IFINTGFLDR TGDEIHTSME AGPMLRKNEM KSSTWLQAYE KSNVAVGLAA
510 520 530 540 550
GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP SPTAATLHAL
560 570 580 590 600
HYHQVNVSAV QSELANDRND YRDDMLQIPV VDHPQWTAEE IQEELDNNCQ
610 620 630 640 650
SILGYVVRWI DQGIGCSKVP DIHNVGLMED RATLRISSQI LANWLHHGIC
660 670 680 690 700
TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLIFRGY
710 720
EQPNGYTEPI LHRRRQEAKA KFAAIQQ
Length:727
Mass (Da):81,148
Last modified:January 31, 2005 - v1
Checksum:iFB5BEA853C8B4CDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75818.1.
RefSeqiYP_147386.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD75818; BAD75818; GK1533.
KEGGigka:GK1533.
PATRICi21964365. VBIGeoKau81518_1650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD75818.1.
RefSeqiYP_147386.1. NC_006510.1.

3D structure databases

ProteinModelPortaliQ5KZR8.
SMRiQ5KZR8. Positions 5-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK1533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD75818; BAD75818; GK1533.
KEGGigka:GK1533.
PATRICi21964365. VBIGeoKau81518_1650.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciGKAU235909:GJO7-1609-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiMASZ_GEOKA
AccessioniPrimary (citable) accession number: Q5KZR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: January 31, 2005
Last modified: March 31, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.