Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q5KZR8 (MASZ_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:GK1533
OrganismGeobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]
Taxonomic identifier235909 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641

Subunit structure

Monomer By similarity. HAMAP MF_00641

Subcellular location

Cytoplasm By similarity HAMAP MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmalate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Malate synthase G HAMAP MF_00641
PRO_1000056902

Sites

Active site3391Proton acceptor By similarity
Active site6301Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5KZR8 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: FB5BEA853C8B4CDB

FASTA72781,148
        10         20         30         40         50         60 
MGEYVQKGNI QVAKVLYDFV NEELLPNSGL DQDKFWSDFG ALISDLTPRN KELLARRDEI 

        70         80         90        100        110        120 
QEKLNEWHKA HRGRFDFNEY KAFLTDIGYL EPEVEDFEIT TDNVDDEIAV QAGPQLVVPL 

       130        140        150        160        170        180 
TNARYALNAA NARWGSLYDA LYGTDAISEE DGAERGSSYN PVRGAKVIAY GRQFLDEAVP 

       190        200        210        220        230        240 
LAQYSHKDAV QYAIVDGQLV VTTEGGATTG LKEPEKFVGF QGDPQHPTAV LLKNNGLHIE 

       250        260        270        280        290        300 
IQIDREHAVG KTDQAGVKDI VLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LFGLVKGDLT 

       310        320        330        340        350        360 
ATFEKNGKIM TRSLNPDRVY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI LHANGEEVHE 

       370        380        390        400        410        420 
GIMDAVITSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS AEVAFANELF DRVEDMLGLE 

       430        440        450        460        470        480 
RNTIKIGVMD EERRTSLNLK NCIYQVRDRI IFINTGFLDR TGDEIHTSME AGPMLRKNEM 

       490        500        510        520        530        540 
KSSTWLQAYE KSNVAVGLAA GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP 

       550        560        570        580        590        600 
SPTAATLHAL HYHQVNVSAV QSELANDRND YRDDMLQIPV VDHPQWTAEE IQEELDNNCQ 

       610        620        630        640        650        660 
SILGYVVRWI DQGIGCSKVP DIHNVGLMED RATLRISSQI LANWLHHGIC TKEQVLETLK 

       670        680        690        700        710        720 
RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLIFRGY EQPNGYTEPI LHRRRQEAKA 


KFAAIQQ

« Hide

References

[1]"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTA426.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000043 Genomic DNA. Translation: BAD75818.1.
RefSeqYP_147386.1. NC_006510.1.

3D structure databases

HSSPHSSP built from PDB template 1N8I based on UniProtKB P0A5J4.
ProteinModelPortalQ5KZR8.
SMRQ5KZR8. Positions 5-723.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3183955.
GenomeReviewsGene locus GK1533 in contig BA000043_GR.
KEGGgka:GK1533.
NMPDRfig|235909.3.peg.382.
PATRIC21964365. VBIGeoKau81518_1650.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG350005.
OMAPKMHGPD.
ProtClustDBPRK02999.

Enzyme and pathway databases

BioCycGKAU235909:GK1533-MONOMER.

Family and domain databases

HAMAPMF_00641. Malate_synth_G.
[Tree]
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
Gene3DG3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KOK01638.
PANTHERPTHR21631:SF1. Malate_synthase. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. Malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_GEOKA
AccessionPrimary (citable) accession number: Q5KZR8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 2005
Last modified: January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents