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Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339GlyoxylateUniRule annotation1
Metal bindingi431MagnesiumUniRule annotation1
Binding sitei431GlyoxylateUniRule annotation1
Metal bindingi459MagnesiumUniRule annotation1
Binding sitei540Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei630Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:GK1533
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569021 – 727Malate synthase GAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei616Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK1533

Structurei

3D structure databases

ProteinModelPortaliQ5KZR8
SMRiQ5KZR8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 125Acetyl-CoA bindingUniRule annotation2
Regioni456 – 459Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C82 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q5KZR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEYVQKGNI QVAKVLYDFV NEELLPNSGL DQDKFWSDFG ALISDLTPRN
60 70 80 90 100
KELLARRDEI QEKLNEWHKA HRGRFDFNEY KAFLTDIGYL EPEVEDFEIT
110 120 130 140 150
TDNVDDEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE
160 170 180 190 200
DGAERGSSYN PVRGAKVIAY GRQFLDEAVP LAQYSHKDAV QYAIVDGQLV
210 220 230 240 250
VTTEGGATTG LKEPEKFVGF QGDPQHPTAV LLKNNGLHIE IQIDREHAVG
260 270 280 290 300
KTDQAGVKDI VLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LFGLVKGDLT
310 320 330 340 350
ATFEKNGKIM TRSLNPDRVY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI
360 370 380 390 400
LHANGEEVHE GIMDAVITSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS
410 420 430 440 450
AEVAFANELF DRVEDMLGLE RNTIKIGVMD EERRTSLNLK NCIYQVRDRI
460 470 480 490 500
IFINTGFLDR TGDEIHTSME AGPMLRKNEM KSSTWLQAYE KSNVAVGLAA
510 520 530 540 550
GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP SPTAATLHAL
560 570 580 590 600
HYHQVNVSAV QSELANDRND YRDDMLQIPV VDHPQWTAEE IQEELDNNCQ
610 620 630 640 650
SILGYVVRWI DQGIGCSKVP DIHNVGLMED RATLRISSQI LANWLHHGIC
660 670 680 690 700
TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLIFRGY
710 720
EQPNGYTEPI LHRRRQEAKA KFAAIQQ
Length:727
Mass (Da):81,148
Last modified:February 1, 2005 - v1
Checksum:iFB5BEA853C8B4CDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA Translation: BAD75818.1
RefSeqiWP_011231029.1, NC_006510.1

Genome annotation databases

EnsemblBacteriaiBAD75818; BAD75818; GK1533
GeneIDi32063441
KEGGigka:GK1533

Similar proteinsi

Entry informationi

Entry nameiMASZ_GEOKA
AccessioniPrimary (citable) accession number: Q5KZR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 1, 2005
Last modified: April 25, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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