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Q5KZR8

- MASZ_GEOKA

UniProt

Q5KZR8 - MASZ_GEOKA

Protein

Malate synthase G

Gene

glcB

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei275 – 2751Acetyl-CoAUniRule annotation
    Binding sitei312 – 3121Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi431 – 4311MagnesiumUniRule annotation
    Binding sitei431 – 4311GlyoxylateUniRule annotation
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei630 – 6301Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciGKAU235909:GJO7-1609-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:GK1533
    OrganismiGeobacillus kaustophilus (strain HTA426)
    Taxonomic identifieri235909 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
    ProteomesiUP000001172: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Malate synthase GPRO_1000056902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi235909.GK1533.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5KZR8.
    SMRiQ5KZR8. Positions 5-723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni456 – 4594Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5KZR8-1 [UniParc]FASTAAdd to Basket

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    MGEYVQKGNI QVAKVLYDFV NEELLPNSGL DQDKFWSDFG ALISDLTPRN    50
    KELLARRDEI QEKLNEWHKA HRGRFDFNEY KAFLTDIGYL EPEVEDFEIT 100
    TDNVDDEIAV QAGPQLVVPL TNARYALNAA NARWGSLYDA LYGTDAISEE 150
    DGAERGSSYN PVRGAKVIAY GRQFLDEAVP LAQYSHKDAV QYAIVDGQLV 200
    VTTEGGATTG LKEPEKFVGF QGDPQHPTAV LLKNNGLHIE IQIDREHAVG 250
    KTDQAGVKDI VLEAAVTTIM DGEDSVAAVD AEDKVLVYRN LFGLVKGDLT 300
    ATFEKNGKIM TRSLNPDRVY KTPDGGELVL PGRSLMFVRN VGHLMTNNAI 350
    LHANGEEVHE GIMDAVITSL IMKHSLIGNT RYLNSRKGSI YIVKPKMHGS 400
    AEVAFANELF DRVEDMLGLE RNTIKIGVMD EERRTSLNLK NCIYQVRDRI 450
    IFINTGFLDR TGDEIHTSME AGPMLRKNEM KSSTWLQAYE KSNVAVGLAA 500
    GFRGRAQIGK GMWAMPDLMA EMLKQKGAQL KAGANTAWVP SPTAATLHAL 550
    HYHQVNVSAV QSELANDRND YRDDMLQIPV VDHPQWTAEE IQEELDNNCQ 600
    SILGYVVRWI DQGIGCSKVP DIHNVGLMED RATLRISSQI LANWLHHGIC 650
    TKEQVLETLK RMAKVVDEQN AGDPNYRPMA PNYDDSVAFQ AACDLIFRGY 700
    EQPNGYTEPI LHRRRQEAKA KFAAIQQ 727
    Length:727
    Mass (Da):81,148
    Last modified:February 1, 2005 - v1
    Checksum:iFB5BEA853C8B4CDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000043 Genomic DNA. Translation: BAD75818.1.
    RefSeqiYP_147386.1. NC_006510.1.

    Genome annotation databases

    EnsemblBacteriaiBAD75818; BAD75818; GK1533.
    GeneIDi3183955.
    KEGGigka:GK1533.
    PATRICi21964365. VBIGeoKau81518_1650.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000043 Genomic DNA. Translation: BAD75818.1 .
    RefSeqi YP_147386.1. NC_006510.1.

    3D structure databases

    ProteinModelPortali Q5KZR8.
    SMRi Q5KZR8. Positions 5-723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 235909.GK1533.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD75818 ; BAD75818 ; GK1533 .
    GeneIDi 3183955.
    KEGGi gka:GK1533.
    PATRICi 21964365. VBIGeoKau81518_1650.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci GKAU235909:GJO7-1609-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
      Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
      Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HTA426.

    Entry informationi

    Entry nameiMASZ_GEOKA
    AccessioniPrimary (citable) accession number: Q5KZR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3