Q5KZN1 (BIOB_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	GK1570

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus kaustophilus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01694
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381401
Sites
	Metal binding	65	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	69	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	72	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	109	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	141	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	201	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	271	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KZN1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: C025951F519D9E1B	FASTA	333	36,518
	10 20 30 40 50 60 MANWLVLAEQ VLGGHELTDE EALAILDCPD EELLLLLQGA YRIRSAYYGN KVKLNMIINA 70 80 90 100 110 120 KSGLCPENCG YCSQSAVSTA PVKTYKMVDK ETLLRGAEEA HRLRIGTYCI VASGRGPSDK 130 140 150 160 170 180 EIDTVVSAVK EIKERFGLKV CACLGILKPE QAARLKEAGV DRYNHNINTS KQHHPNITTS 190 200 210 220 230 240 HTYDDRVRTV ETVKEAGLSP CSGVIIGMKE TKRDVVDMAR SLRALDADSI PVNFLHAIDG 250 260 270 280 290 300 TPLAGTNELN PRYCLKVLAL FRYMNPTKEI RIAGGREVNL RSLQPLGLYA ANSIFVGDYL 310 320 330 TTAGQEKSAD YQMLEDLGFE IEFAPAPQAG VVS

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD75855.1.
RefSeq	YP_147423.1. NC_006510.1.
3D structure databases
ProteinModelPortal	Q5KZN1.
ModBase	Search...
Protein-protein interaction databases
STRING	235909.GK1570.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD75855; BAD75855; GK1570.
GeneID	3185941.
KEGG	gka:GK1570.
PATRIC	21964445. VBIGeoKau81518_1690.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239958.
KO	K01012.
OMA	MKETKQD.
ProtClustDB	PRK06256.
Enzyme and pathway databases
BioCyc	GKAU235909:GJO7-1646-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_GEOKA

Accession

Primary (citable) accession number: Q5KZN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 1, 2005
Last modified:	May 29, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program