Reviewed, UniProtKB/Swiss-Prot Q5KYR4 (IOLA2_GEOKA)

Names and origin

Protein names

Recommended name:
    Methylmalonate semialdehyde dehydrogenase [acylating] 2
      Short name=MMSA dehydrogenase 2
      Short name=MMSDH 2
      Short name=MSDH 2
    EC=1.2.1.27
Alternative name(s):
    Malonate semialdehyde dehydrogenase [acetylating] 2
      Short name=MSA dehydrogenase 2
    EC=1.2.1.18

Gene names

Name:	iolA2
Ordered Locus Names:	GK1887

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts malonate semialdehyde (MSA) and methylmalonate semialdehyde (MMSA) into acetyl-CoA and propanoyl-CoA, respectively By similarity.
Catalytic activity	3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. HAMAP MF_01670 2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. HAMAP MF_01670
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 7/7. HAMAP MF_01670
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. IolA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from electronic annotation. Source: HAMAP methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Methylmalonate semialdehyde dehydrogenase [acylating] 2 HAMAP MF_01670		PRO_0000352340
Regions
	Nucleotide binding	177 – 181	5	NAD By similarity
Sites
	Active site	285	1	Nucleophile By similarity
	Binding site	385	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KYR4-1 [UniParc]. Last modified October 14, 2008. Version 2. Checksum: 77C7333D2B9EF600	FASTA	484	53,035
	10 20 30 40 50 60 MTTGVQTLKN YIGGQWVESR SGKTEAVPNP ATGEVLAYVP ISSREELDEA VRAAKEAFKT 70 80 90 100 110 120 WRKTPVPRRA RILFKYQQLL VEHWEELARL VTLENGKSYN EAYGEVQRGI ECVEFAAGAP 130 140 150 160 170 180 TLMMGRQLPD IATGIESGMY RYPIGVVGGI TPFNFPMMVP CWMFPLAIAC GNTFVLKPSE 190 200 210 220 230 240 RTPMLANRLA ELFKEAGLPD GVLNIVHGAH DVVNGLLEHP DVKAISFVGS QPVGEYVYKT 250 260 270 280 290 300 AAAHGKRVQA LTGAKNHSIV MPDADLNVAV REIINAAFGS AGERCMAASV VVAVGEIADE 310 320 330 340 350 360 LVEKLVAAAN ELKIGNGLEE SVFLGPVIRE AHKQRTVKYI ELGEKEGAIL VRDGRKDAAV 370 380 390 400 410 420 QDNGYFIGPT IFDRVTTDMT IWKDEIFAPV LSIVRVETLD EAIEVANKSP FANGACIYTR 430 440 450 460 470 480 DGGNVRKFRE EIDAGMLGVN LGVPAPMAFF PFSGWKNSFY GDLHANGMDG VEFYTRKKMM TSRW

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD76172.1. Different initiation.
RefSeq	YP_147740.1.
3D structure databases
SMR	Q5KYR4. Positions 9-488.
ModBase	Search...
Genome annotation databases
GeneID	3183410.
GenomeReviews	Gene locus GK1887 in contig BA000043_GR.
KEGG	gka:GK1887.
NMPDR	fig|235909.3.peg.736.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5KYR4.
Family and domain databases
HAMAP	MF_01670. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLA2_GEOKA

Accession

Primary (citable) accession number: Q5KYR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	June 16, 2009
This is version 29 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)