Reviewed, UniProtKB/Swiss-Prot Q5KYR0 (IOLD_GEOKA)

Names and origin

Protein names

Recommended name:
    3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
      Short name=THcHDO hydrolase
    EC=3.7.1.n2

Gene names

Name:	iolD
Ordered Locus Names:	GK1891

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the cleavage of the C1-C2 bond of 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy-glucuronate (5DG) By similarity.
Catalytic activity	3,5/4-trihydroxycyclohexa-1,2-dione + H2O = 5-deoxy-glucuronic acid. HAMAP MF_01669
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into acetyl-CoA; acetyl-CoA from myo-inositol: step 3/7. HAMAP MF_01669
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding NAD Thiamine pyrophosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP thiamin pyrophosphate binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase HAMAP MF_01669		PRO_0000352542
Regions
	Region	442 – 522	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	493	1	Magnesium By similarity
	Metal binding	520	1	Magnesium By similarity
	Binding site	65	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KYR0-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: B9646307DA6408B7	FASTA	644	71,689
	10 20 30 40 50 60 MKTIRLTTAQ ALVKFLNQQY VEFDGHIQKF VKGIFTIFGH GNVLGLGQAL EEDPGELEVY 70 80 90 100 110 120 QGRNEQGMAH AAIAFAKQKH RRQIMACTSS VGPGAANMVT AAATASANHI PVLLLPGDTF 130 140 150 160 170 180 ATRQPDPVLQ QIEHWHDLTV STNDAFRAVS KYWDRISRPE QLMSAMIQAM RVLTDPANTG 190 200 210 220 230 240 AVTIALPQDV QGEAYDFPES FFQKRIHRIE RRVPAKAAIH EAVELIRRKK KPIIICGGGV 250 260 270 280 290 300 RYSEAAEELK QFAEKFHIPY GETQAGKSAV ESAHPYNLGG IGVTGNLAAN IIAKEADLVI 310 320 330 340 350 360 GIGTRYTDFT TGSKQLFQHP EVEFLTINVS VFDACKLDAV RVVADAKLAL LALTEELEKI 370 380 390 400 410 420 GYRSGYTNEI DIAKKAWEEE LNRLHHVRYR QSGFQPEVAG HLDEVLTEYS EFFDSSLTQT 430 440 450 460 470 480 EVIGAINEWI DDDAIIIGAA GSLPGDLQRM WVARRPNTYH MEYGYSCMGY EIAGALGVKM 490 500 510 520 530 540 AEPDKEVYAM VGDGSYLMLH SELVTSIQEK KKINVLLFDN GGFGCINNLQ MGHGMGSFAT 550 560 570 580 590 600 EFRYRNEETG RLDGSLIPID FAQSAAGYGV KTYKVHTLEQ LKEALEDAKQ QSVSTLIDIK 610 620 630 640 VLPKTMTHDY ESWWHVGVAE VSRNPKIAEA YKRKEQQLQR ARKY

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD76176.1.
RefSeq	YP_147744.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3185975.
GenomeReviews	Gene locus GK1891 in contig BA000043_GR.
KEGG	gka:GK1891.
NMPDR	fig|235909.3.peg.740.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5KYR0.
OMA	Q5KYR0. GELEVYQ.
Family and domain databases
HAMAP	MF_01669. [Tree]
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOLD_GEOKA

Accession

Primary (citable) accession number: Q5KYR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	February 1, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)