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Reviewed, UniProtKB/Swiss-Prot Q5KYM1 (URE1_GEOKA)

Last modified November 3, 2009. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha
Gene names
Name: ureC
Ordered Locus Names: GK1930
OrganismGeobacillus kaustophilus [Complete proteome] [HAMAP]
Taxonomic identifier1462 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

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Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953

Cofactor

Binds 2 nickel ions per subunit By similarity.

Pathway

Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953

Subunit structure

Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamylation allows a single lysine to coordinate two nickel ions By similarity.

Sequence similarities

Belongs to the urease family.

Contains 1 urease domain.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
Nickel
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processurea metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionnickel ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

urease activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 569569Urease subunit alpha HAMAP MF_01953
PRO_0000234156

Regions

Domain131 – 569439Urease

Sites

Active site3221Proton donor By similarity
Metal binding1361Nickel 2 By similarity
Metal binding1381Nickel 2 By similarity
Metal binding2191Nickel 1; via carbamate group By similarity
Metal binding2191Nickel 2; via carbamate group By similarity
Metal binding2481Nickel 1 By similarity
Metal binding2741Nickel 1 By similarity
Metal binding3621Nickel 2 By similarity
Binding site2211Substrate By similarity

Amino acid modifications

Modified residue2191N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q5KYM1-1 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 42FC32C3B4F41BDD

FASTA56961,400
        10         20         30         40         50         60 
MSFSMSRRQY ADMFGPTTGD CIRLADTDLW IEIEHDYTVY GDEVKFGGGK VIRDGMGQHP 

        70         80         90        100        110        120 
LATRDEAVDL VLTNAVIVDY TGIYKADIGI KDGNIAAIGK AGNPLLMDGV NIVIGASTEV 

       130        140        150        160        170        180 
IAAEGKIVTA GGVDAHIHFI CPQQIETALS SGITTMIGGG TGPATGTNAT TCTPGEWNIY 

       190        200        210        220        230        240 
RMLEAAEAFP MNIGFLGKGN ASAKEPIAEQ VRAGAIGLKL HEDWGTTAAA IDACLRVADE 

       250        260        270        280        290        300 
YDVQVAIHTD TLNEGGFVEH TLKAINGRVI HTYHTEGAGG GHAPDIMKVA SFPNILPSST 

       310        320        330        340        350        360 
NPTRPYTKNT LDEHLDMLMV CHHLDPSVPE DIAFADSRIR KETIAAEDIL HDIGAFSMIS 

       370        380        390        400        410        420 
SDSQAMGRVG EVILRTWQTA DKMKKQFGRL PEETGRGDNV RVKRYVAKYT INPAITHGIA 

       430        440        450        460        470        480 
EYVGSVEVGK FADLVVWHPA FFGVKPELVI KGGMIAYSVM GDPNASIPTP QPALYRPMFA 

       490        500        510        520        530        540 
SYGAAIAKTS ITFLSKAAFE RGIPDKLGLH KIVKPVGNIR SLTKNDMVFN NAMLQIDVDP 

       550        560 
QTYEVKVDGR LITCEPAEVV AMAQRYFLF

« Hide

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References

[1]"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTA426.

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Cross-references

Sequence databases

BA000043 Genomic DNA. Translation: BAD76215.1.
RefSeqYP_147783.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3184685.
GenomeReviewsGene locus GK1930 in contig BA000043_GR.
KEGGgka:GK1930.
NMPDRfig|235909.3.peg.779.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ5KYM1.
OMASHIHFIC.

Enzyme and pathway databases

BioCycGKAU235909:GK1930-MON.
BRENDA3.5.1.5. 281547.

Family and domain databases

HAMAPMF_01953.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR011612. Urease_alpha_N.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR017952. Urease_asu_core.
IPR017950. Urease_asu_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSPR01752. UREASE.
TIGRFAMsTIGR01792. urease_alph. 1 hit.
PROSITEPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameURE1_GEOKA
AccessionPrimary (citable) accession number: Q5KYM1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: February 1, 2005
Last modified: November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents