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Protein

Glutamate 5-kinase

Gene

proB

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9ATPUniRule annotation1
Binding sitei49SubstrateUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Binding sitei148Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 169ATPUniRule annotation2
Nucleotide bindingi210 – 216ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:GK2048
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001096741 – 374Glutamate 5-kinaseAdd BLAST374

Interactioni

Protein-protein interaction databases

STRINGi235909.GK2048.

Structurei

3D structure databases

ProteinModelPortaliQ5KYA3.
SMRiQ5KYA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini276 – 354PUAUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIHRDHW.
OrthoDBiPOG091H00OF.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5KYA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQRVVVKI GSSSLTDPKG GLCHDKLFDH VEAIAYLKQL GHDVILITSG
60 70 80 90 100
AVAAGFGPLG YPARPTTIAG KQAAAAVGQS LLMQAYSSAF AQFGFTAAQL
110 120 130 140 150
LLTRSDFYSR ERFRNLFATI TTLLENGAVP IINENDSVSI EELTFGDNDM
160 170 180 190 200
LSALVAGFLH ADALILLTDI NGLYDANPKT NPQAKKYAFL PEITDEMIEA
210 220 230 240 250
AGGIGSAVGT GGMRSKLLAA RKALSFGVSV FIGTGSGREK LADILAGKGD
260 270 280 290 300
GTYIGVPFPK QMQMRKQWIA YHAPVAGMIT VDSGAEEALL MRGKSLLPAG
310 320 330 340 350
VTAVSGDFHA MDVVDVVNEK GITIGRGQVY YAAADLKKVK GRPSEEARQY
360 370
SYLHRPEVIH RDNWVTLRKE SVSK
Length:374
Mass (Da):40,081
Last modified:February 1, 2005 - v1
Checksum:iD964FE8B4E68B89B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76333.1.
RefSeqiWP_011231534.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD76333; BAD76333; GK2048.
GeneIDi3184072.
KEGGigka:GK2048.
PATRICi21965485. VBIGeoKau81518_2198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76333.1.
RefSeqiWP_011231534.1. NC_006510.1.

3D structure databases

ProteinModelPortaliQ5KYA3.
SMRiQ5KYA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK2048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD76333; BAD76333; GK2048.
GeneIDi3184072.
KEGGigka:GK2048.
PATRICi21965485. VBIGeoKau81518_2198.

Phylogenomic databases

eggNOGiENOG4105CGT. Bacteria.
COG0263. LUCA.
HOGENOMiHOG000246368.
KOiK00931.
OMAiVIHRDHW.
OrthoDBiPOG091H00OF.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00359.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPiMF_00456. ProB. 1 hit.
InterProiView protein in InterPro
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
PfamiView protein in Pfam
PF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
PIRSFiPIRSF000729. GK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SMARTiView protein in SMART
SM00359. PUA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR01027. proB. 1 hit.
PROSITEiView protein in PROSITE
PS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPROB_GEOKA
AccessioniPrimary (citable) accession number: Q5KYA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 1, 2005
Last modified: March 15, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.