Reviewed, UniProtKB/Swiss-Prot Q5KY94 (AROD_GEOKA)

Names and origin

Protein names

Recommended name:
    3-dehydroquinate dehydratase
      Short name=3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	GK2057

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 257

257

3-dehydroquinate dehydratase HAMAP MF_00214

PRO_0000325524

Sites

Active site

147

1

Proton acceptor By similarity

Active site

174

1

Schiff-base intermediate with substrate By similarity

Secondary structure

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257

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Beta strand

8 – 10

3

Beta strand

13 – 19

7

Beta strand

21 – 26

6

Helix

31 – 43

13

Beta strand

47 – 52

6

Helix

53 – 55

3

Turn

57 – 60

4

Helix

62 – 75

14

Beta strand

76 – 78

3

Beta strand

81 – 84

4

Turn

88 – 91

4

Helix

99 – 112

14

Beta strand

116 – 121

6

Helix

122 – 126

5

Helix

127 – 137

11

Beta strand

141 – 150

10

Helix

155 – 167

13

Beta strand

171 – 177

7

Helix

182 – 198

17

Beta strand

203 – 207

5

Turn

209 – 212

4

Helix

213 – 218

6

Helix

219 – 222

4

Beta strand

226 – 228

3

Beta strand

230 – 233

4

Helix

242 – 256

15

Sequences

	Sequence		Length	Mass (Da)
	Q5KY94-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: D988D5A96B791D6A	FASTA	257	28,564
	10 20 30 40 50 60 MNISPKAIKV RNIWIGGTEP CICAPVVGED DRKVLREAEE VCRKQPDLLE WRADFFRAID 70 80 90 100 110 120 DQERVLATAN GLRNIAGEIP ILFTIRSERE GGQPIPLNEA EVRRLIEAIC RSGAIDLVDY 130 140 150 160 170 180 ELAYGERIAD VRRMTEECSV WLVVSRHYFD GTPRKETLLA DMRQAERYGA DIAKVAVMPK 190 200 210 220 230 240 SPEDVLVLLQ ATEEARRELA IPLITMAMGG LGAITRLAGW LFGSAVTFAV GNQSSAPGQI 250 PIDDVRTVLS ILQTYSR

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BA000043 Genomic DNA. Translation: BAD76342.1.

RefSeq

YP_147910.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YR1	X-ray	2.00	A/B	1-257	[»]

ModBase

Search...

Genome annotation databases

GeneID

3186501.

GenomeReviews

Gene locus GK2057 in contig BA000043_GR.

KEGG

gka:GK2057.

NMPDR

fig|235909.3.peg.906.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q5KY94.

OMA

THEYSAA.

Enzyme and pathway databases

BioCyc

GKAU235909:GK2057-MON.

BRENDA

4.2.1.10. 281547.

Family and domain databases

HAMAP

MF_00214.
[Tree]

InterPro

IPR018508. 3-dehydroquinate_DH_AS.
IPR013785. Aldolase_TIM.
IPR001381. DHquinase_I.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.

Pfam

PF01487. DHquinase_I. 1 hit.
[Graphical view]

ProDom

PD005337. DHquinase_I. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR01093. aroD. 1 hit.

PROSITE

PS01028. DEHYDROQUINASE_I. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AROD_GEOKA

Accession

Primary (citable) accession number: Q5KY94

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 1, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)