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Protein

NADPH dehydrogenase

Gene

namA

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.UniRule annotation

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.UniRule annotation

Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281SubstrateUniRule annotation
Binding sitei60 – 601FMN; via amide nitrogenUniRule annotation
Binding sitei102 – 1021FMNUniRule annotation
Binding sitei215 – 2151FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi23 – 264FMNUniRule annotation
Nucleotide bindingi307 – 3082FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Detoxification

Keywords - Ligandi

Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenaseUniRule annotation (EC:1.6.99.1UniRule annotation)
Gene namesi
Name:namAUniRule annotation
Ordered Locus Names:GK2332
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340NADPH dehydrogenasePRO_0000216121Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK2332.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi14 – 229Combined sources
Beta strandi33 – 353Combined sources
Helixi39 – 5012Combined sources
Beta strandi54 – 6411Combined sources
Helixi65 – 673Combined sources
Beta strandi68 – 703Combined sources
Beta strandi73 – 753Combined sources
Helixi81 – 9414Combined sources
Beta strandi98 – 1047Combined sources
Helixi107 – 1093Combined sources
Beta strandi112 – 1143Combined sources
Beta strandi117 – 1215Combined sources
Helixi136 – 15621Combined sources
Beta strandi159 – 1657Combined sources
Helixi170 – 1756Combined sources
Turni177 – 1793Combined sources
Beta strandi187 – 1893Combined sources
Helixi190 – 20718Combined sources
Beta strandi212 – 2187Combined sources
Helixi228 – 2303Combined sources
Helixi231 – 24010Combined sources
Beta strandi245 – 2495Combined sources
Turni262 – 2654Combined sources
Helixi266 – 27510Combined sources
Beta strandi280 – 2856Combined sources
Helixi289 – 2979Combined sources
Beta strandi302 – 3065Combined sources
Helixi308 – 3125Combined sources
Helixi316 – 3238Combined sources
Helixi332 – 3343Combined sources
Turni335 – 3373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GR7X-ray2.30A/B1-340[»]
3GR8X-ray2.50A/B1-340[»]
ProteinModelPortaliQ5KXG9.
SMRiQ5KXG9. Positions 5-338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5KXG9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 1674Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116232.
KOiK00354.
OMAiNWPLHAR.
OrthoDBiPOG091H03LJ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KXG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV
60 70 80 90 100
GQVGLIIVEA TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG
110 120 130 140 150
IQLAHAGRKS QVPGEIIAPS AVPFDDSSPT PKEMTKADIE ETVQAFQNGA
160 170 180 190 200
RRAKEAGFDV IEIHAAHGYL INEFLSPLSN RRQDEYGGSP ENRYRFLGEV
210 220 230 240 250
IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE QGVDLVDVSS
260 270 280 290 300
GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR
310 320 330 340
ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF
Length:340
Mass (Da):37,680
Last modified:February 1, 2005 - v1
Checksum:iA386C16F7EC573EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeqiWP_011231814.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD76617; BAD76617; GK2332.
GeneIDi3186211.
KEGGigka:GK2332.
PATRICi21966089. VBIGeoKau81518_2493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeqiWP_011231814.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3GR7X-ray2.30A/B1-340[»]
3GR8X-ray2.50A/B1-340[»]
ProteinModelPortaliQ5KXG9.
SMRiQ5KXG9. Positions 5-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK2332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD76617; BAD76617; GK2332.
GeneIDi3186211.
KEGGigka:GK2332.
PATRICi21966089. VBIGeoKau81518_2493.

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116232.
KOiK00354.
OMAiNWPLHAR.
OrthoDBiPOG091H03LJ.

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2427-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5KXG9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAMA_GEOKA
AccessioniPrimary (citable) accession number: Q5KXG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.