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Reviewed, UniProtKB/Swiss-Prot Q5KXG9 (NAMA_GEOKA)

Last modified June 16, 2009. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase
Gene names
Name: namA
Ordered Locus Names: GK2332
OrganismGeobacillus kaustophilus [Complete proteome] [HAMAP]
Taxonomic identifier1462 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

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Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.

Catalytic activity

NADPH + acceptor = NADP+ + reduced acceptor. HAMAP MF_01614

Cofactor

FMN By similarity.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

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Ontologies

Keywords
   LigandFMN
Flavoprotein
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionFMN binding

Inferred from electronic annotation. Source: InterPro

NADPH dehydrogenase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340NADPH dehydrogenase HAMAP MF_01614
PRO_0000216121

Regions

Nucleotide binding23 – 275FMN By similarity

Sites

Binding site281Substrate By similarity
Binding site1641Substrate By similarity
Binding site1671Substrate By similarity
Binding site2151FMN By similarity
Binding site3081FMN By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q5KXG9-1 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: A386C16F7EC573EE

FASTA34037,680
        10         20         30         40         50         60 
MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV GQVGLIIVEA 

        70         80         90        100        110        120 
TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG IQLAHAGRKS QVPGEIIAPS 

       130        140        150        160        170        180 
AVPFDDSSPT PKEMTKADIE ETVQAFQNGA RRAKEAGFDV IEIHAAHGYL INEFLSPLSN 

       190        200        210        220        230        240 
RRQDEYGGSP ENRYRFLGEV IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE 

       250        260        270        280        290        300 
QGVDLVDVSS GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR 

       310        320        330        340 
ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF

« Hide

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References

[1]"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTA426.

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Cross-references

Sequence databases

BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeqYP_148185.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3186211.
GenomeReviewsGene locus GK2332 in contig BA000043_GR.
KEGGgka:GK2332.
NMPDRfig|235909.3.peg.1192.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ5KXG9.
OMAQ5KXG9. RGLTVRN.

Enzyme and pathway databases

BioCycGKAU235909:GK2332-MON.
BRENDA1.6.99.1. 281547.

Family and domain databases

HAMAPMF_01614.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PfamPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNAMA_GEOKA
AccessionPrimary (citable) accession number: Q5KXG9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 1, 2005
Last modified: June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents