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Protein

NADPH dehydrogenase

Gene

namA

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of the double bond of an array of alpha,beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes.UniRule annotation

Catalytic activityi

NADPH + acceptor = NADP+ + reduced acceptor.UniRule annotation

Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28SubstrateUniRule annotation1
Binding sitei60FMN; via amide nitrogenUniRule annotation1
Binding sitei102FMNUniRule annotation1
Binding sitei215FMNUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi23 – 26FMNUniRule annotation4
Nucleotide bindingi307 – 308FMNUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Detoxification

Keywords - Ligandi

Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH dehydrogenaseUniRule annotation (EC:1.6.99.1UniRule annotation)
Gene namesi
Name:namAUniRule annotation
Ordered Locus Names:GK2332
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002161211 – 340NADPH dehydrogenaseAdd BLAST340

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK2332.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 11Combined sources3
Beta strandi14 – 22Combined sources9
Beta strandi33 – 35Combined sources3
Helixi39 – 50Combined sources12
Beta strandi54 – 64Combined sources11
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi73 – 75Combined sources3
Helixi81 – 94Combined sources14
Beta strandi98 – 104Combined sources7
Helixi107 – 109Combined sources3
Beta strandi112 – 114Combined sources3
Beta strandi117 – 121Combined sources5
Helixi136 – 156Combined sources21
Beta strandi159 – 165Combined sources7
Helixi170 – 175Combined sources6
Turni177 – 179Combined sources3
Beta strandi187 – 189Combined sources3
Helixi190 – 207Combined sources18
Beta strandi212 – 218Combined sources7
Helixi228 – 230Combined sources3
Helixi231 – 240Combined sources10
Beta strandi245 – 249Combined sources5
Turni262 – 265Combined sources4
Helixi266 – 275Combined sources10
Beta strandi280 – 285Combined sources6
Helixi289 – 297Combined sources9
Beta strandi302 – 306Combined sources5
Helixi308 – 312Combined sources5
Helixi316 – 323Combined sources8
Helixi332 – 334Combined sources3
Turni335 – 337Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GR7X-ray2.30A/B1-340[»]
3GR8X-ray2.50A/B1-340[»]
ProteinModelPortaliQ5KXG9.
SMRiQ5KXG9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5KXG9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 167Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116232.
KOiK00354.
OMAiNWPLHAR.
OrthoDBiPOG091H03LJ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KXG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV
60 70 80 90 100
GQVGLIIVEA TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG
110 120 130 140 150
IQLAHAGRKS QVPGEIIAPS AVPFDDSSPT PKEMTKADIE ETVQAFQNGA
160 170 180 190 200
RRAKEAGFDV IEIHAAHGYL INEFLSPLSN RRQDEYGGSP ENRYRFLGEV
210 220 230 240 250
IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE QGVDLVDVSS
260 270 280 290 300
GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR
310 320 330 340
ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF
Length:340
Mass (Da):37,680
Last modified:February 1, 2005 - v1
Checksum:iA386C16F7EC573EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeqiWP_011231814.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD76617; BAD76617; GK2332.
GeneIDi3186211.
KEGGigka:GK2332.
PATRICi21966089. VBIGeoKau81518_2493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeqiWP_011231814.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GR7X-ray2.30A/B1-340[»]
3GR8X-ray2.50A/B1-340[»]
ProteinModelPortaliQ5KXG9.
SMRiQ5KXG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK2332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD76617; BAD76617; GK2332.
GeneIDi3186211.
KEGGigka:GK2332.
PATRICi21966089. VBIGeoKau81518_2493.

Phylogenomic databases

eggNOGiENOG4105CCY. Bacteria.
COG1902. LUCA.
HOGENOMiHOG000116232.
KOiK00354.
OMAiNWPLHAR.
OrthoDBiPOG091H03LJ.

Miscellaneous databases

EvolutionaryTraceiQ5KXG9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01614. NamA. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR023663. NADPH_DH.
IPR001155. OxRdtase_FMN_N.
[Graphical view]
PfamiPF00724. Oxidored_FMN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAMA_GEOKA
AccessioniPrimary (citable) accession number: Q5KXG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.