Reviewed, UniProtKB/Swiss-Prot Q5KXG9 (NAMA_GEOKA)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	GK2332

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP(+) + reduced acceptor.
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	NADPH dehydrogenase		PRO_0000216121
Regions
	Nucleotide binding	23 – 27	5	FMN By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	215	1	FMN By similarity
	Binding site	308	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KXG9-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: A386C16F7EC573EE	FASTA	340	37,680
	10 20 30 40 50 60 MNTMLFSPYT IRGLTLKNRI VMSPMCMYSC DTKDGAVRTW HKIHYPARAV GQVGLIIVEA 70 80 90 100 110 120 TGVTPQGRIS ERDLGIWSDD HIAGLRELVG LVKEHGAAIG IQLAHAGRKS QVPGEIIAPS 130 140 150 160 170 180 AVPFDDSSPT PKEMTKADIE ETVQAFQNGA RRAKEAGFDV IEIHAAHGYL INEFLSPLSN 190 200 210 220 230 240 RRQDEYGGSP ENRYRFLGEV IDAVREVWDG PLFVRISASD YHPDGLTAKD YVPYAKRMKE 250 260 270 280 290 300 QGVDLVDVSS GAIVPARMNV YPGYQVPFAE LIRREADIPT GAVGLITSGW QAEEILQNGR 310 320 330 340 ADLVFLGREL LRNPYWPYAA ARELGAKISA PVQYERGWRF

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD76617.1.
RefSeq	YP_148185.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3186211.
GenomeReviews	Gene locus GK2332 in contig BA000043_GR.
KEGG	gka:GK2332.
NMPDR	fig|235909.3.peg.1192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5KXG9.
Enzyme and pathway databases
BioCyc	GKAU235909:GK2332-MON.
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_GEOKA

Accession

Primary (citable) accession number: Q5KXG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	February 1, 2005
Last modified:	November 25, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)