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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei62 – 621ShikimateUniRule annotation
Active sitei66 – 661Proton acceptorUniRule annotation
Binding sitei78 – 781NADPUniRule annotation
Binding sitei87 – 871ShikimateUniRule annotation
Binding sitei102 – 1021ShikimateUniRule annotation
Binding sitei218 – 2181NADP; via carbonyl oxygenUniRule annotation
Binding sitei220 – 2201ShikimateUniRule annotation
Binding sitei241 – 2411NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi151 – 1566NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2619-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:GK2524
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
Proteomesi
  • UP000001172 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276Shikimate dehydrogenase (NADP(+))PRO_0000431402Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi235909.GK2524.

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 119Combined sources
Helixi17 – 2711Combined sources
Beta strandi32 – 387Combined sources
Helixi44 – 5411Combined sources
Beta strandi58 – 614Combined sources
Turni66 – 694Combined sources
Helixi70 – 723Combined sources
Beta strandi74 – 763Combined sources
Helixi78 – 836Combined sources
Beta strandi88 – 925Combined sources
Beta strandi95 – 995Combined sources
Helixi102 – 11312Combined sources
Beta strandi122 – 1265Combined sources
Helixi130 – 14011Combined sources
Turni141 – 1433Combined sources
Beta strandi145 – 1506Combined sources
Helixi154 – 16310Combined sources
Beta strandi166 – 1683Combined sources
Helixi174 – 1796Combined sources
Helixi181 – 1833Combined sources
Beta strandi185 – 1895Combined sources
Beta strandi214 – 2174Combined sources
Beta strandi221 – 2244Combined sources
Helixi226 – 2338Combined sources
Beta strandi237 – 2393Combined sources
Helixi242 – 25716Combined sources
Helixi263 – 27513Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EGGX-ray2.25A/B1-276[»]
ProteinModelPortaliQ5KWX7.
SMRiQ5KWX7. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5KWX7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 173Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiTTVDGAW.
OrthoDBiPOG091H02DY.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5KWX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVYGLIGF PVEHSLSPLM HNDAFARLGI PARYHLFSVE PGQVGAAIAG
60 70 80 90 100
VRALGIAGVN VTIPHKLAVI PFLDEVDEHA RRIGAVNTII NNDGRLVGYN
110 120 130 140 150
TDGLGYVQAL EEEMNITLDG KRILVIGAGG GARGIYFSLL STAAERIDMA
160 170 180 190 200
NRTVEKAERL VREGDERRSA YFSLAEAETR LAEYDIIINT TSVGMHPRVE
210 220 230 240 250
VQPLSLERLR PGVIVSDIIY NPLETKWLKE AKARGARVQN GVGMLVYQGA
260 270
LAFEKWTGQW PDVNRMKQLV IEALRR
Length:276
Mass (Da):30,427
Last modified:February 1, 2005 - v1
Checksum:i1054EC5195AD3B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76809.1.
RefSeqiWP_011232003.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD76809; BAD76809; GK2524.
GeneIDi3186474.
KEGGigka:GK2524.
PATRICi21966509. VBIGeoKau81518_2703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD76809.1.
RefSeqiWP_011232003.1. NC_006510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EGGX-ray2.25A/B1-276[»]
ProteinModelPortaliQ5KWX7.
SMRiQ5KWX7. Positions 1-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK2524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD76809; BAD76809; GK2524.
GeneIDi3186474.
KEGGigka:GK2524.
PATRICi21966509. VBIGeoKau81518_2703.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiTTVDGAW.
OrthoDBiPOG091H02DY.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciGKAU235909:GJO7-2619-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5KWX7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_GEOKA
AccessioniPrimary (citable) accession number: Q5KWX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.