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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei62ShikimateUniRule annotation1
Active sitei66Proton acceptorUniRule annotation1
Binding sitei78NADPUniRule annotation1
Binding sitei87ShikimateUniRule annotation1
Binding sitei102ShikimateUniRule annotation1
Binding sitei218NADP; via carbonyl oxygenUniRule annotation1
Binding sitei220ShikimateUniRule annotation1
Binding sitei241NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 156NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:GK2524
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000001172 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004314021 – 276Shikimate dehydrogenase (NADP(+))Add BLAST276

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi235909.GK2524

Structurei

Secondary structure

1276
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Helixi17 – 27Combined sources11
Beta strandi32 – 38Combined sources7
Helixi44 – 54Combined sources11
Beta strandi58 – 61Combined sources4
Turni66 – 69Combined sources4
Helixi70 – 72Combined sources3
Beta strandi74 – 76Combined sources3
Helixi78 – 83Combined sources6
Beta strandi88 – 92Combined sources5
Beta strandi95 – 99Combined sources5
Helixi102 – 113Combined sources12
Beta strandi122 – 126Combined sources5
Helixi130 – 140Combined sources11
Turni141 – 143Combined sources3
Beta strandi145 – 150Combined sources6
Helixi154 – 163Combined sources10
Beta strandi166 – 168Combined sources3
Helixi174 – 179Combined sources6
Helixi181 – 183Combined sources3
Beta strandi185 – 189Combined sources5
Beta strandi214 – 217Combined sources4
Beta strandi221 – 224Combined sources4
Helixi226 – 233Combined sources8
Beta strandi237 – 239Combined sources3
Helixi242 – 257Combined sources16
Helixi263 – 275Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EGGX-ray2.25A/B1-276[»]
ProteinModelPortaliQ5KWX7
SMRiQ5KWX7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5KWX7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 17Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiFGNPIKH
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5KWX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVYGLIGF PVEHSLSPLM HNDAFARLGI PARYHLFSVE PGQVGAAIAG
60 70 80 90 100
VRALGIAGVN VTIPHKLAVI PFLDEVDEHA RRIGAVNTII NNDGRLVGYN
110 120 130 140 150
TDGLGYVQAL EEEMNITLDG KRILVIGAGG GARGIYFSLL STAAERIDMA
160 170 180 190 200
NRTVEKAERL VREGDERRSA YFSLAEAETR LAEYDIIINT TSVGMHPRVE
210 220 230 240 250
VQPLSLERLR PGVIVSDIIY NPLETKWLKE AKARGARVQN GVGMLVYQGA
260 270
LAFEKWTGQW PDVNRMKQLV IEALRR
Length:276
Mass (Da):30,427
Last modified:February 1, 2005 - v1
Checksum:i1054EC5195AD3B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA Translation: BAD76809.1
RefSeqiWP_011232003.1, NC_006510.1

Genome annotation databases

EnsemblBacteriaiBAD76809; BAD76809; GK2524
KEGGigka:GK2524

Entry informationi

Entry nameiAROE_GEOKA
AccessioniPrimary (citable) accession number: Q5KWX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health