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Q5KWV9 (MTNN_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Short name=MTA/SAH nucleosidase
Short name=MTAN
EC=3.2.2.9
Alternative name(s):
5'-methylthioadenosine nucleosidase
Short name=MTA nucleosidase
S-adenosylhomocysteine nucleosidase
Short name=AdoHcy nucleosidase
Short name=SAH nucleosidase
Short name=SRH nucleosidase
Gene names
Name:mtnN
Ordered Locus Names:GK2542
OrganismGeobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]
Taxonomic identifier235909 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684

Catalytic activity

S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684

S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684

Sequence similarities

Belongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2352355'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
PRO_0000359302

Regions

Region174 – 1752Substrate binding By similarity

Sites

Active site121Proton acceptor By similarity
Binding site781Substrate; via amide nitrogen By similarity
Binding site1981Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5KWV9 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 64BF687993D93DA3

FASTA23525,114
        10         20         30         40         50         60 
MKAAIIGAME EEVAILRSRM EGCEETVIAG CEFSKGRLDG VEAVLLKSGI GKVNAAMGTT 

        70         80         90        100        110        120 
LLLDHFRPDF VINTGSAGGF LPSLRVGDLV ISEEVVHHDV DVTAFGYAYG QVPGLPARYR 

       130        140        150        160        170        180 
ADEALVEAAK QAAARLDGLQ AVTGLIATGD SFMNDPKRVE FVRGQFPELC AVEMEAAAIA 

       190        200        210        220        230 
QVCVQFGTPF VIIRALSDIA GEESGVSFEQ FLETAAKHSA ELVLSMLSVM KQKRY

« Hide

References

[1]"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HTA426.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000043 Genomic DNA. Translation: BAD76827.1.
RefSeqYP_148395.1. NC_006510.1.

3D structure databases

HSSPHSSP built from PDB template 1JYS based on UniProtKB P0AF12.
ProteinModelPortalQ5KWV9.
SMRQ5KWV9. Positions 1-228.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3185392.
GenomeReviewsGene locus GK2542 in contig BA000043_GR.
KEGGgka:GK2542.
NMPDRfig|235909.3.peg.2634.
PATRIC21966545. VBIGeoKau81518_2721.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG367723.
OMALEHFPTM.
ProtClustDBPRK05584.

Enzyme and pathway databases

BioCycGKAU235909:GK2542-MONOMER.

Family and domain databases

HAMAPMF_01684. Salvage_tnN.
[Tree]
InterProIPR010049. MTA_SAH_Nsdase.
IPR018017. Nucleoside_phosphorylase.
IPR000845. Nucleoside_phosphorylase_d.
[Graphical view]
KOK01243.
PANTHERPTHR21234. PNP_UDP. 1 hit.
PTHR21234:SF6. PTHR21234:SF6. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTNN_GEOKA
AccessionPrimary (citable) accession number: Q5KWV9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: February 1, 2005
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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