Q5KWI5 (RNPH_GEOKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	GK2666

Organism

Geobacillus kaustophilus (strain HTA426) [Complete proteome] [HAMAP]

Taxonomic identifier

235909 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus › Geobacillus kaustophilus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.
Sequence caution	The sequence BAD76951.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Ribonuclease PH HAMAP-Rule MF_00564		PRO_0000139894

Sequences

	Sequence		Length	Mass (Da)
	Q5KWI5 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 0A738D51349B2131	FASTA	256	27,939
	10 20 30 40 50 60 MRADGRSSCE LRPVHIHPHY MKHAEGSVLI EVGDTKVICT ATVEEKVPSF MRGGGKGWIT 70 80 90 100 110 120 AEYGMLPRAT EQRNVREASK GKVSGRTMEI QRLIGRALRS VVDLEQLGER TIWIDCDVIQ 130 140 150 160 170 180 ADGGTRTASI TGAYVALVLA LAKLVEEGRL EALPIRDFLA ATSVGIDPEH GVILDLDYNE 190 200 210 220 230 240 DARAKVDMNV VMTGAGQFVE IQGTGEEAVF SRAELDELLE AAQIGIEQLI AVQRRALGEW 250 AARIGEKKEA AEEDTE

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD76951.1. Different initiation.
RefSeq	YP_148519.2. NC_006510.1.
3D structure databases
ProteinModelPortal	Q5KWI5.
SMR	Q5KWI5. Positions 1-239.
ModBase	Search...
Protein-protein interaction databases
STRING	235909.GK2666.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD76951; BAD76951; GK2666.
GeneID	3185465.
KEGG	gka:GK2666.
PATRIC	21966801. VBIGeoKau81518_2849.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0689.
HOGENOM	HOG000229516.
KO	K00989.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Enzyme and pathway databases
BioCyc	GKAU235909:GJO7-2761-MONOMER.
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_GEOKA

Accession

Primary (citable) accession number: Q5KWI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program