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Protein

DNA polymerase I

Gene

polA

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei617CTPCombined sources1
Binding sitei631CTPCombined sources1
Metal bindingi655MagnesiumCombined sources1
Metal bindingi655ManganeseCombined sources1
Metal bindingi656Magnesium; via carbonyl oxygenCombined sources1
Metal bindingi656Manganese; via carbonyl oxygenCombined sources1
Binding sitei684CTPCombined sources1
Binding sitei704CTPCombined sources1
Binding sitei708CTPCombined sources1
Binding sitei712CTPCombined sources1
Binding sitei716CTPCombined sources1
Metal bindingi832MagnesiumCombined sources1
Metal bindingi832ManganeseCombined sources1
Binding sitei832CTPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi471 – 475CTPCombined sources5
Nucleotide bindingi658 – 660CTPCombined sources3
Nucleotide bindingi769 – 770CTPCombined sources2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, DNA-directed DNA polymeraseUniRule annotationImported, Hydrolase, NucleaseSAAS annotation, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repairUniRule annotation, DNA replicationUniRule annotation
LigandMagnesiumCombined sources, ManganeseCombined sources, Metal-bindingCombined sources, Nucleotide-bindingCombined sources

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IUniRule annotation (EC:2.7.7.7UniRule annotation)
Gene namesi
Name:polAUniRule annotationImported
Ordered Locus Names:GK2730Imported
OrganismiGeobacillus kaustophilus (strain HTA426)Imported
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
Proteomesi
  • UP000001172 Componenti: Chromosome

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.UniRule annotation

Protein-protein interaction databases

STRINGi235909.GK2730

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NJWX-ray1.90A299-878[»]
1UA0X-ray2.10A299-878[»]
2HHUX-ray1.80A300-878[»]
2HHVX-ray1.55A300-878[»]
2HHWX-ray1.88A/D299-878[»]
2HHXX-ray2.26A300-878[»]
2HVIX-ray1.98A/D300-878[»]
3HP6X-ray1.81A/D299-878[»]
3PV8X-ray1.52A/D287-878[»]
3PX0X-ray1.73A/D287-878[»]
3PX4X-ray1.58A/D287-878[»]
3PX6X-ray1.59A/D287-878[»]
4DQIX-ray1.69A/D287-878[»]
4DQPX-ray1.74A/D287-878[»]
4DQQX-ray1.59A/D287-878[»]
4DQRX-ray1.95A/D287-878[»]
4DQSX-ray1.66A287-878[»]
4DS4X-ray1.68A/D287-878[»]
4DS5X-ray1.68A/D287-878[»]
4DSEX-ray1.67A/D287-878[»]
4DSFX-ray1.66A/D287-878[»]
4E0DX-ray1.58A287-878[»]
4EZ6X-ray1.64A/D287-878[»]
4EZ9X-ray1.64A/D287-878[»]
4F2RX-ray1.63A/D287-878[»]
4F2SX-ray1.65A/D287-878[»]
4F3OX-ray1.57A/D287-878[»]
4F4KX-ray1.60A/D287-878[»]
4F8RX-ray1.64A/D287-878[»]
ProteinModelPortaliQ5KWC1
SMRiQ5KWC1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5KWC1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 26453EXOcInterPro annotationAdd BLAST260
Domaini302 – 4683'-5' exonucleaseInterPro annotationAdd BLAST167
Domaini635 – 842POLAcInterPro annotationAdd BLAST208

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C2M Bacteria
COG0258 LUCA
COG0749 LUCA
HOGENOMiHOG000020998
KOiK02335
OMAiETGRVHT
OrthoDBiPOG091H00PR

Family and domain databases

CDDicd09898 H3TH_53EXO, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR002562 3'-5'_exonuclease_dom
IPR020046 5-3_exonucl_a-hlix_arch_N
IPR036279 5-3_exonuclease_C_sf
IPR002421 5-3_exonuclease_N
IPR019760 DNA-dir_DNA_pol_A_CS
IPR001098 DNA-dir_DNA_pol_A_palm_dom
IPR020045 DNA_polI_H3TH
IPR018320 DNA_polymerase_1
IPR002298 DNA_polymerase_A
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10133 PTHR10133, 1 hit
PfamiView protein in Pfam
PF01367 5_3_exonuc, 1 hit
PF02739 5_3_exonuc_N, 1 hit
PF00476 DNA_pol_A, 1 hit
PRINTSiPR00868 DNAPOLI
SMARTiView protein in SMART
SM00474 35EXOc, 1 hit
SM00475 53EXOc, 1 hit
SM00279 HhH2, 1 hit
SM00482 POLAc, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF53098 SSF53098, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR00593 pola, 1 hit
PROSITEiView protein in PROSITE
PS00447 DNA_POLYMERASE_A, 1 hit

Sequencei

Sequence statusi: Complete.

Q5KWC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLKKKLVLI DGSSVAYRAF FALPLLHNDK GIHTNAVYGF TMMLNKILAE
60 70 80 90 100
EEPTHMLVAF DAGKTTFRHE AFQEYKGGRQ QTPPELSEQF PLLRELLRAY
110 120 130 140 150
RIPAYELENY EADDIIGTLA ARAEQEGFEV KVISGDRDLT QLASPHVTVD
160 170 180 190 200
ITKKGITDIE PYTPETVREK YGLTPEQIVD LKGLMGDKSD NIPGVPGIGE
210 220 230 240 250
KTAVKLLRQF GTVENVLASI DEIKGEKLKE TLRQHREMAL LSKKLAAIRR
260 270 280 290 300
DAPVELSLDD IVYQGEDREK VVALFKELGF QSFLEKMESP SSEEEKPLAK
310 320 330 340 350
MAFTLADRVT EEMLADKAAL VVEVVEENYH DAPIVGIAVV NEHGRFFLRP
360 370 380 390 400
ETALADPQFV AWLGDETKKK SMFDSKRAAV ALKWKGIELC GVSFDLLLAA
410 420 430 440 450
YLLDPAQGVD DVAAAAKMKQ YEAVRPDEAV YGKGAKRAVP DEPVLAEHLV
460 470 480 490 500
RKAAAIWELE RPFLDELRRN EQDRLLVELE QPLSSILAEM EFAGVKVDTK
510 520 530 540 550
RLEQMGKELA EQLGTVEQRI YELAGQEFNI NSPKQLGVIL FEKLQLPVLK
560 570 580 590 600
KTKTGYSTSA DVLEKLAPYH EIVENILHYR QLGKLQSTYI EGLLKVVRPD
610 620 630 640 650
TKKVHTIFNQ ALTQTGRLSS TEPNLQNIPI RLEEGRKIRQ AFVPSESDWL
660 670 680 690 700
IFAADYSQIE LRVLAHIAED DNLMEAFRRD LDIHTKTAMD IFQVSEDEVT
710 720 730 740 750
PNMRRQAKAV NFGIVYGISD YGLAQNLNIS RKEAAEFIER YFESFPGVKR
760 770 780 790 800
YMENIVQEAK QKGYVTTLLH RRRYLPDITS RNFNVRSFAE RMAMNTPIQG
810 820 830 840 850
SAADIIKKAM IDLNARLKEE RLQARLLLQV HDELILEAPK EEMERLCRLV
860 870
PEVMEQAVTL RVPLKVDYHY GSTWYDAK
Length:878
Mass (Da):99,646
Last modified:February 1, 2005 - v1
Checksum:iD50E58940CE21B01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA Translation: BAD77015.1
RefSeqiWP_011232204.1, NC_006510.1

Genome annotation databases

EnsemblBacteriaiBAD77015; BAD77015; GK2730
KEGGigka:GK2730
PATRICifig|235909.7.peg.2914

Similar proteinsi

Entry informationi

Entry nameiQ5KWC1_GEOKA
AccessioniPrimary (citable) accession number: Q5KWC1
Entry historyiIntegrated into UniProtKB/TrEMBL: February 1, 2005
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

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