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Q5KW28

- TRMB_GEOKA

UniProt

Q5KW28 - TRMB_GEOKA

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trmB, GK2823
Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionine By similarity
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei96 – 961S-adenosyl-L-methionine By similarity
Active sitei118 – 1181 By similarity
Binding sitei118 – 1181S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221Substrate By similarity
Binding sitei154 – 1541Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2918-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:GK2823
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000229164Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi235909.GK2823.

Structurei

3D structure databases

ProteinModelPortaliQ5KW28.
SMRiQ5KW28. Positions 9-209.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Interaction with RNA Reviewed prediction
Regioni191 – 1944Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KW28-1 [UniParc]FASTAAdd to Basket

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MRLRNKPWAK DKIAAYPQYV IPDPETKRGR WRELFGHDQP LHVEIGTGKG    50
KFITEMAKLH PDVNFIGIEL YPSVLVSALD KLIESGLANV KLLNANAKDL 100
TAFFADGEVS RIYLNFSDPW PKKRHEKRRL TYRDFLALYD RILAEDGDIH 150
LKTDNQSFFE YSLVSLSQYG FVLASVQLDL HQSGMTDNVM TEYEEKFSAK 200
GNRIYRCEAV RPPRRSS 217
Length:217
Mass (Da):25,042
Last modified:February 1, 2005 - v1
Checksum:iB797B5832BD4F5BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000043 Genomic DNA. Translation: BAD77108.1.
RefSeqiYP_148676.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD77108; BAD77108; GK2823.
GeneIDi3184862.
KEGGigka:GK2823.
PATRICi21967129. VBIGeoKau81518_3013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000043 Genomic DNA. Translation: BAD77108.1 .
RefSeqi YP_148676.1. NC_006510.1.

3D structure databases

ProteinModelPortali Q5KW28.
SMRi Q5KW28. Positions 9-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 235909.GK2823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD77108 ; BAD77108 ; GK2823 .
GeneIDi 3184862.
KEGGi gka:GK2823.
PATRICi 21967129. VBIGeoKau81518_3013.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci GKAU235909:GJO7-2918-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiTRMB_GEOKA
AccessioniPrimary (citable) accession number: Q5KW28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 1, 2005
Last modified: June 11, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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