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Q5KW28

- TRMB_GEOKA

UniProt

Q5KW28 - TRMB_GEOKA

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
Active sitei118 – 1181By similarity
Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221SubstrateUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2918-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:GK2823
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferasePRO_0000229164Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi235909.GK2823.

Structurei

3D structure databases

ProteinModelPortaliQ5KW28.
SMRiQ5KW28. Positions 9-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Interaction with RNAUniRule annotation
Regioni191 – 1944Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KW28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLRNKPWAK DKIAAYPQYV IPDPETKRGR WRELFGHDQP LHVEIGTGKG
60 70 80 90 100
KFITEMAKLH PDVNFIGIEL YPSVLVSALD KLIESGLANV KLLNANAKDL
110 120 130 140 150
TAFFADGEVS RIYLNFSDPW PKKRHEKRRL TYRDFLALYD RILAEDGDIH
160 170 180 190 200
LKTDNQSFFE YSLVSLSQYG FVLASVQLDL HQSGMTDNVM TEYEEKFSAK
210
GNRIYRCEAV RPPRRSS
Length:217
Mass (Da):25,042
Last modified:February 1, 2005 - v1
Checksum:iB797B5832BD4F5BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD77108.1.
RefSeqiYP_148676.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD77108; BAD77108; GK2823.
GeneIDi3184862.
KEGGigka:GK2823.
PATRICi21967129. VBIGeoKau81518_3013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD77108.1 .
RefSeqi YP_148676.1. NC_006510.1.

3D structure databases

ProteinModelPortali Q5KW28.
SMRi Q5KW28. Positions 9-209.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 235909.GK2823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD77108 ; BAD77108 ; GK2823 .
GeneIDi 3184862.
KEGGi gka:GK2823.
PATRICi 21967129. VBIGeoKau81518_3013.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci GKAU235909:GJO7-2918-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiTRMB_GEOKA
AccessioniPrimary (citable) accession number: Q5KW28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 1, 2005
Last modified: October 1, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3