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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Geobacillus kaustophilus (strain HTA426)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2937-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:GK2842
OrganismiGeobacillus kaustophilus (strain HTA426)
Taxonomic identifieri235909 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus
ProteomesiUP000001172 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Leucine--tRNA ligasePRO_0000152019Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi235909.GK2842.

Structurei

3D structure databases

ProteinModelPortaliQ5KW09.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KW09-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFNHREIEQ KWQDYWEKNK TFRTPDDDDK PKFYVLDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT
110 120 130 140 150
QKNIDNFRRQ IKSLGFSYDW DREINTTDPN YYKWTQWIFL KLYEKGLAYM
160 170 180 190 200
DEVPVNWCPA LGTVLANEEV INGRSERGGH PVIRKPMRQW MLKITAYADR
210 220 230 240 250
LLEDLEELDW PESIKEMQRN WIGRSEGAEI EFAVHGHDET FTVFTTRPDT
260 270 280 290 300
LFGATYTVLA PEHPLVEKIT TPEQKPAVDA YLKEIQSKSD LERTDLAKEK
310 320 330 340 350
TGVFTGAYAI HPVTGDRLPI WIADYVLMSY GTGAIMAVPA HDERDYEFAK
360 370 380 390 400
KFHLPMKEVV AGGDIEKEPY TGDGEHINSE FLNGLNKQEA IDKMIAWLEE
410 420 430 440 450
HGKGRKKVSY RLRDWLFSRQ RYWGEPIPII HWEDGTMTPV PEEELPLVLP
460 470 480 490 500
KTDEIRPSGT GESPLANIEE WVNVVDPKTG KKGRRETNTM PQWAGSCWYY
510 520 530 540 550
LRYIDPHNDK QLADPEKLKK WLPVDVYIGG AEHAVLHLLY ARFWHKFLYD
560 570 580 590 600
LGIVPTKEPF QKLFNQGMIL GENNEKMSKS KGNVVNPDDI IESHGADTLR
610 620 630 640 650
LYEMFMGPLE ASIAWSTKGL DGARRFLDRV WRLFVTENGE LNPNIVDEPA
660 670 680 690 700
NDTLERIYHQ TVKKVTEDYE SLRFNTAISQ LMVFINEAYK AEQMKKEYME
710 720 730 740 750
GFVKLLSPVC PHIGEELWQK LGHTDTIAYE PWPTYDETKL VEDVVEIVVQ
760 770 780 790 800
INGKVRSRLH VPVDLPKEAL EERALADEKI KEQLEGKTVR KVIAVPGKLV

NIVAN
Length:805
Mass (Da):92,875
Last modified:February 1, 2005 - v1
Checksum:iC5D51D58D9C78270
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD77127.1.
RefSeqiWP_011232314.1. NC_006510.1.

Genome annotation databases

EnsemblBacteriaiBAD77127; BAD77127; GK2842.
GeneIDi3185286.
KEGGigka:GK2842.
PATRICi21967171. VBIGeoKau81518_3034.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000043 Genomic DNA. Translation: BAD77127.1.
RefSeqiWP_011232314.1. NC_006510.1.

3D structure databases

ProteinModelPortaliQ5KW09.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi235909.GK2842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD77127; BAD77127; GK2842.
GeneIDi3185286.
KEGGigka:GK2842.
PATRICi21967171. VBIGeoKau81518_3034.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciGKAU235909:GJO7-2937-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus."
    Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I.
    Nucleic Acids Res. 32:6292-6303(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HTA426.

Entry informationi

Entry nameiSYL_GEOKA
AccessioniPrimary (citable) accession number: Q5KW09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: February 1, 2005
Last modified: July 22, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.