Reviewed, UniProtKB/Swiss-Prot Q5KVY8 (LUXS_GEOKA)

Names and origin

Protein names

Recommended name:
    S-ribosylhomocysteine lyase
    EC=4.4.1.21
Alternative name(s):
    Autoinducer-2 production protein luxS
    AI-2 synthesis protein

Gene names

Name:	luxS
Ordered Locus Names:	GK2863

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity.
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091
Cofactor	Binds 1 iron ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the luxS family.

Ontologies

Keywords
Biological process	Autoinducer synthesis Quorum sensing
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	S-ribosylhomocysteine lyase HAMAP MF_00091		PRO_0000172225
Sites
	Metal binding	55	1	Iron By similarity
	Metal binding	59	1	Iron By similarity
	Metal binding	127	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KVY8-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: B1B92F8E94716549	FASTA	158	17,668
	10 20 30 40 50 60 MPSPVESFEL DHCAVKAPYV RHCGVHKVGS DGVVNKFDIR FCQPNKEAMD PAAIHTLEHL 70 80 90 100 110 120 LAYTLRRHAA KYDHFDIIDI SPMGCQTGFY LVVSGSPTVD EIIDLLEEAM KDALAATEVP 130 140 150 AATERQCGQA KLHDLEAAKQ LMRFWLSQEK EELKKVFG

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000043 Genomic DNA. Translation: BAD77148.1.
RefSeq	YP_148716.1.
3D structure databases
SMR	Q5KVY8. Positions 5-158.
ModBase	Search...
Genome annotation databases
GeneID	3184765.
GenomeReviews	Gene locus GK2863 in contig BA000043_GR.
KEGG	gka:GK2863.
NMPDR	fig|235909.3.peg.2703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5KVY8.
OMA	VRIAKTM.
Enzyme and pathway databases
BioCyc	GKAU235909:GK2863-MON.
BRENDA	4.4.1.21. 281547.
Family and domain databases
HAMAP	MF_00091. [Tree]
InterPro	IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. LuxS. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

LUXS_GEOKA

Accession

Primary (citable) accession number: Q5KVY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	February 1, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)