Reviewed, UniProtKB/Swiss-Prot Q5KUG7 (MURA2_GEOKA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 2
    UDP-N-acetylglucosamine enolpyruvyl transferase 2
      Short name=EPT 2

Gene names

Name:	murA2
Ordered Locus Names:	GK3384

Organism

Geobacillus kaustophilus [Complete proteome] [HAMAP]

Taxonomic identifier

1462 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 HAMAP MF_00111		PRO_0000231205
Sites
	Active site	116	1	Proton donor By similarity
	Binding site	116	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KUG7-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 89D19B8D1738AAE4	FASTA	428	45,536
	10 20 30 40 50 60 MDKMKIIGGD RLRGTIKVSG AKNSAVALIP AAILADSPVT IEGLPDISDV HILGSLIEEI 70 80 90 100 110 120 GGSFSFDGKE AVIDPTNMVS MPLPNGKVKK LRASYYLMGA MLGRFKRAVV GLPGGCHLGP 130 140 150 160 170 180 RPIDQHIKGF EALGATVTNE QGAIYLRAEE LRGARIFLDV VSVGATINIM LAAVRAKGRT 190 200 210 220 230 240 IIENAAKEPE IIDVATLLSN MGAKIKGAGT DVIRIDGVEK LSGCRHAIIP DRIEAGTYMI 250 260 270 280 290 300 AAAATNGEVV VDNVIPQHVE SLTAKLREMG VRVETGEDQI LVCGTDVLKA VDVKTLVYPG 310 320 330 340 350 360 FPTDLQQPFT ALLTKANGTS VVTDTIYSAR FKHVDELRRM NANVKVEGRS AIVTGPVKLQ 370 380 390 400 410 420 GAKVKASDLR AGAALVIAGL MAEGVTEITG VEHIDRGYSN LVEKLSDIGA TIWREKMTDE EIEQVKNA

References

[1]

"Thermoadaptation trait revealed by the genome sequence of thermophilic Geobacillus kaustophilus." Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H., Matsui S., Uchiyama I. Nucleic Acids Res. 32:6292-6303(2004) [PubMed: 15576355] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HTA426.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000043 Genomic DNA. Translation: BAD77669.1.
RefSeq	YP_149237.1.
3D structure databases
SMR	Q5KUG7. Positions 1-414.
ModBase	Search...
Genome annotation databases
GeneID	3183648.
GenomeReviews	Gene locus GK3384 in contig BA000043_GR.
KEGG	gka:GK3384.
NMPDR	fig|235909.3.peg.3425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG482701.
OMA	DNVIPTH.
Enzyme and pathway databases
BioCyc	GKAU235909:GK3384-MONOMER.
BRENDA	2.5.1.7. 281547.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA2_GEOKA

Accession

Primary (citable) accession number: Q5KUG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)