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Protein

N-acylethanolamine-hydrolyzing acid amidase

Gene

Naaa

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N-palmitoylethanolamine > N-myristoylethanolamine > N-stearoylethanolamine > N-oleoylethanolamine > N-linoleoylethanolamine > N-arachidonoylethanolamine.1 Publication

Enzyme regulationi

Stimulated by DTT and Triton X-100.1 Publication

Kineticsi

  1. KM=35 µM for N-palmitoylethanolamine1 Publication

    pH dependencei

    Optimum pH is 5 with N-palmitoylethanolamine as substrate.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei131NucleophileBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BRENDAi3.5.1.4. 5301.
    ReactomeiR-RNO-112310. Neurotransmitter Release Cycle.

    Protein family/group databases

    MEROPSiC89.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acylethanolamine-hydrolyzing acid amidase (EC:3.5.1.-)
    Alternative name(s):
    N-acylsphingosine amidohydrolase-like
    Short name:
    ASAH-like protein
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Naaa
    Synonyms:Asahl
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 14

    Organism-specific databases

    RGDi1307267. Naaa.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL2034805.
    GuidetoPHARMACOLOGYi1402.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 33By similarityAdd BLAST33
    ChainiPRO_000000232034 – 362N-acylethanolamine-hydrolyzing acid amidaseAdd BLAST329
    ChainiPRO_000041965434 – 130N-acylethanolamine-hydrolyzing acid amidase subunit alphaAdd BLAST97
    ChainiPRO_0000419655131 – 349N-acylethanolamine-hydrolyzing acid amidase subunit betaAdd BLAST219

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi42N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi338N-linked (GlcNAc...)Sequence analysis1

    Post-translational modificationi

    Autoproteolytic cleavage in the acidic lumen of the lysosome activates the enzyme.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ5KTC7.
    PRIDEiQ5KTC7.

    Expressioni

    Tissue specificityi

    Expressed in brain, cecum, colon, heart, ileum, kidney, liver, lung, spleen, stomach, submaxillary gland, testis and thymus.1 Publication

    Gene expression databases

    BgeeiENSRNOG00000002273.
    GenevisibleiQ5KTC7. RN.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit, non-covalently linked.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003102.

    Chemistry databases

    BindingDBiQ5KTC7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5KTC7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acid ceramidase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG410IJHJ. Eukaryota.
    ENOG4111HJY. LUCA.
    GeneTreeiENSGT00530000063548.
    HOGENOMiHOG000007253.
    HOVERGENiHBG050586.
    InParanoidiQ5KTC7.
    KOiK13720.
    OMAiRFLPQPF.
    OrthoDBiEOG091G0E3H.
    PhylomeDBiQ5KTC7.
    TreeFamiTF313219.

    Family and domain databases

    InterProiIPR016699. Acid_ceramidase-like.
    IPR029130. Acid_ceramidase_N.
    IPR029132. CBAH/NAAA_C.
    [Graphical view]
    PfamiPF02275. CBAH. 1 hit.
    PF15508. NAAA-beta. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017632. Acid_ceramidase-like. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5KTC7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGTPAIRAAC HGAHLALALL LLLSLSDPWL WATAPGTPPL FNVSLDAAPE
    60 70 80 90 100
    LRWLPMLQHY DPDFVRAAVA QVIGDRVPQW ILEMIGEIVQ KVESFLPQPF
    110 120 130 140 150
    TSEIRGICDY LNLSLAEGVL VNLAYEASAF CTSIVAQDSQ GRIYHGRNLD
    160 170 180 190 200
    YPFGNALRKL TADVQFVKNG QIVFTATTFV GYVGLWTGQS PHKFTISGDE
    210 220 230 240 250
    RDKGWWWENM IAALSLGHSP ISWLIRKTLT ESEDFEAAVY TLAKTPLIAD
    260 270 280 290 300
    VYYIVGGTSP QEGVVITRDR GGPADIWPLD PLNGAWFRVE TNYDHWEPVP
    310 320 330 340 350
    KRDDRRTPAI KALNATGQAH LSLETLFQVL SVFPVYNNYT IYTTVMSAAE
    360
    PDKYMTMIRN PS
    Length:362
    Mass (Da):40,313
    Last modified:February 15, 2005 - v1
    Checksum:iA9B6AB03F1668FA4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB162193 mRNA. Translation: BAD88529.1.
    BC105771 mRNA. Translation: AAI05772.1.
    RefSeqiNP_001010967.1. NM_001010967.2.
    UniGeneiRn.101943.

    Genome annotation databases

    EnsembliENSRNOT00000003102; ENSRNOP00000003102; ENSRNOG00000002273.
    GeneIDi497009.
    KEGGirno:497009.
    UCSCiRGD:1307267. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB162193 mRNA. Translation: BAD88529.1.
    BC105771 mRNA. Translation: AAI05772.1.
    RefSeqiNP_001010967.1. NM_001010967.2.
    UniGeneiRn.101943.

    3D structure databases

    ProteinModelPortaliQ5KTC7.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000003102.

    Chemistry databases

    BindingDBiQ5KTC7.
    ChEMBLiCHEMBL2034805.
    GuidetoPHARMACOLOGYi1402.

    Protein family/group databases

    MEROPSiC89.002.

    Proteomic databases

    PaxDbiQ5KTC7.
    PRIDEiQ5KTC7.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000003102; ENSRNOP00000003102; ENSRNOG00000002273.
    GeneIDi497009.
    KEGGirno:497009.
    UCSCiRGD:1307267. rat.

    Organism-specific databases

    CTDi27163.
    RGDi1307267. Naaa.

    Phylogenomic databases

    eggNOGiENOG410IJHJ. Eukaryota.
    ENOG4111HJY. LUCA.
    GeneTreeiENSGT00530000063548.
    HOGENOMiHOG000007253.
    HOVERGENiHBG050586.
    InParanoidiQ5KTC7.
    KOiK13720.
    OMAiRFLPQPF.
    OrthoDBiEOG091G0E3H.
    PhylomeDBiQ5KTC7.
    TreeFamiTF313219.

    Enzyme and pathway databases

    BRENDAi3.5.1.4. 5301.
    ReactomeiR-RNO-112310. Neurotransmitter Release Cycle.

    Miscellaneous databases

    PROiQ5KTC7.

    Gene expression databases

    BgeeiENSRNOG00000002273.
    GenevisibleiQ5KTC7. RN.

    Family and domain databases

    InterProiIPR016699. Acid_ceramidase-like.
    IPR029130. Acid_ceramidase_N.
    IPR029132. CBAH/NAAA_C.
    [Graphical view]
    PfamiPF02275. CBAH. 1 hit.
    PF15508. NAAA-beta. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017632. Acid_ceramidase-like. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNAAA_RAT
    AccessioniPrimary (citable) accession number: Q5KTC7
    Secondary accession number(s): Q3KRD3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: February 15, 2005
    Last modified: September 7, 2016
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.