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Q5KSL6

- DGKK_HUMAN

UniProt

Q5KSL6 - DGKK_HUMAN

Protein

Diacylglycerol kinase kappa

Gene

DGKK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).1 Publication

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

    Enzyme regulationi

    Inhibited in response to H2O2.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri327 – 37751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri398 – 44952Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: HGNC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. diacylglycerol metabolic process Source: HGNC
    3. intracellular signal transduction Source: HGNC
    4. platelet activation Source: Reactome
    5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
    6. response to oxidative stress Source: HGNC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase kappa (EC:2.7.1.107)
    Short name:
    DAG kinase kappa
    Alternative name(s):
    142 kDa diacylglycerol kinase
    Diglyceride kinase kappa
    Short name:
    DGK-kappa
    Gene namesi
    Name:DGKK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Unplaced

    Organism-specific databases

    HGNCiHGNC:32395. DGKK.

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: HGNC

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi78 – 781Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). 1 Publication
    Mutagenesisi1075 – 10751Y → F: Does not affect phosphorylation. 1 Publication

    Organism-specific databases

    PharmGKBiPA142671978.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12711271Diacylglycerol kinase kappaPRO_0000239368Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781Phosphotyrosine1 Publication

    Post-translational modificationi

    Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ5KSL6.
    PRIDEiQ5KSL6.

    PTM databases

    PhosphoSiteiQ5KSL6.

    Expressioni

    Tissue specificityi

    Expressed in testis, and to a lesser extent in placenta.1 Publication

    Gene expression databases

    CleanExiHS_DGKK.
    GenevestigatoriQ5KSL6.

    Interactioni

    Subunit structurei

    Does not form homooligomers.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5KSL6.
    SMRiQ5KSL6. Positions 383-449.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati48 – 5141
    Repeati52 – 5542
    Repeati56 – 5943
    Repeati60 – 6344
    Repeati64 – 6745
    Repeati68 – 7146
    Repeati72 – 7547
    Repeati76 – 7948
    Repeati80 – 8349
    Repeati84 – 87410
    Repeati88 – 91411
    Repeati92 – 95412
    Repeati96 – 99413
    Repeati100 – 103414
    Repeati104 – 107415
    Repeati108 – 111416
    Repeati112 – 115417
    Repeati116 – 119418
    Repeati120 – 123419
    Repeati124 – 127420
    Repeati128 – 131421
    Repeati132 – 135422
    Repeati136 – 139423
    Repeati140 – 143424
    Repeati144 – 147425
    Repeati148 – 151426
    Repeati152 – 155427
    Repeati156 – 159428
    Repeati160 – 163429
    Repeati164 – 167430
    Repeati168 – 171431
    Repeati172 – 175432
    Repeati176 – 179433
    Domaini216 – 30994PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini487 – 622136DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni48 – 17913233 X 4 AA approximate tandem repeats of E-P-A-PAdd
    BLAST
    Regioni1199 – 126870Required for localization to the plasma membraneAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1269 – 12713PDZ-binding

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri327 – 37751Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri398 – 44952Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG051346.
    InParanoidiQ5KSL6.
    OMAiGSVQMAM.
    PhylomeDBiQ5KSL6.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5KSL6-1 [UniParc]FASTAAdd to Basket

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    MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS     50
    PEPIPEPCPE LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE 100
    PAPEPATEPA PEPAPEPATE SAPEPTPEPA LESVPEPAPE LTPEVAPELA 150
    PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC LLQCPVDTRE RGLKTSPSPS 200
    PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV QGQKLYFAHH 250
    PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW 300
    INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA 350
    LSRDAIICEV CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ 400
    WVEGNMPVSS QCAVCHESCG SYQRLQDFRC LWCNSTVHDD CRRRFSKECC 450
    FRSHRSSVIP PTALSDPKGD GQLVVSSDFW NLDWSSACSC PLLIFINSKS 500
    GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA RFRILVCGGD 550
    GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD 600
    ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE 650
    SAATELNKIL KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI 700
    VSVILKSDLM YDRLSVLIDV LAEEAAATSA EKSATEYADS SKADRKPFIP 750
    QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV EQALDEESRQ TISVKNFSST 800
    FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL DHLHFTPESI 850
    RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT 900
    KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN 950
    TATTEYEAPA IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI 1000
    DGEEGIPVQV DGEAWIQRPG LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH 1050
    TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH LARLAENLIS KLNDLSKIHQ 1100
    HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL SRNDAVDVTF 1150
    SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI 1200
    FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH 1250
    RRHREDEAEG DDPLTPSRSQ L 1271
    Length:1,271
    Mass (Da):141,829
    Last modified:February 15, 2005 - v1
    Checksum:i1EB8BD12B1992DDB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti1118 – 11181D → N.
    Corresponds to variant rs4074320 [ dbSNP | Ensembl ].
    VAR_048859

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB183864 mRNA. Translation: BAD86792.1.
    BC137319 mRNA. Translation: AAI37320.1.
    BC137320 mRNA. Translation: AAI37321.1.
    RefSeqiNP_001013764.1. NM_001013742.3.
    UniGeneiHs.38132.
    Hs.631770.

    Genome annotation databases

    GeneIDi139189.
    KEGGihsa:139189.
    UCSCiuc010njr.2. human.

    Polymorphism databases

    DMDMi74708075.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB183864 mRNA. Translation: BAD86792.1 .
    BC137319 mRNA. Translation: AAI37320.1 .
    BC137320 mRNA. Translation: AAI37321.1 .
    RefSeqi NP_001013764.1. NM_001013742.3.
    UniGenei Hs.38132.
    Hs.631770.

    3D structure databases

    ProteinModelPortali Q5KSL6.
    SMRi Q5KSL6. Positions 383-449.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q5KSL6.

    Polymorphism databases

    DMDMi 74708075.

    Proteomic databases

    MaxQBi Q5KSL6.
    PRIDEi Q5KSL6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 139189.
    KEGGi hsa:139189.
    UCSCi uc010njr.2. human.

    Organism-specific databases

    CTDi 139189.
    GeneCardsi GC0XM050128.
    HGNCi HGNC:32395. DGKK.
    MIMi 300837. gene.
    neXtProti NX_Q5KSL6.
    PharmGKBi PA142671978.
    GenAtlasi Search...

    Phylogenomic databases

    HOVERGENi HBG051346.
    InParanoidi Q5KSL6.
    OMAi GSVQMAM.
    PhylomeDBi Q5KSL6.

    Enzyme and pathway databases

    Reactomei REACT_2202. Effects of PIP2 hydrolysis.

    Miscellaneous databases

    GenomeRNAii 139189.
    NextBioi 83910.
    PROi Q5KSL6.
    SOURCEi Search...

    Gene expression databases

    CleanExi HS_DGKK.
    Genevestigatori Q5KSL6.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa."
      Imai S., Kai M., Yasuda S., Kanoh H., Sakane F.
      J. Biol. Chem. 280:39870-39881(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-78, MUTAGENESIS OF TYR-78 AND TYR-1075.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiDGKK_HUMAN
    AccessioniPrimary (citable) accession number: Q5KSL6
    Secondary accession number(s): B2RP91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome X
      Human chromosome X: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3