Reviewed, UniProtKB/Swiss-Prot Q5KSL6 (DGKK_HUMAN)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase kappa
      Short name=DAG kinase kappa
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase kappa
      Short name=DGK-kappa
    142 kDa diacylglycerol kinase

Gene names

Name:

DGKK

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Ref.1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1
Enzyme regulation	Inhibited in response to H2O2. Ref.1
Subunit structure	Does not form homooligomers.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Ref.1
Tissue specificity	Expressed in testis, and to a lesser extent in placenta. Ref.1
Post-translational modification	Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2. Ref.1
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro diacylglycerol metabolic process Ref.1 Inferred from direct assay. Source: HGNC intracellular signaling cascade Ref.1 Inferred from direct assay. Source: HGNC response to oxidative stress Ref.1 Inferred from direct assay. Source: HGNC
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Ref.1 Inferred from direct assay. Source: HGNC
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Ref.1 Inferred from direct assay. Source: HGNC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1271	1271	Diacylglycerol kinase kappa		PRO_0000239368
Regions
	Repeat	48 – 51	4	1
	Repeat	52 – 55	4	2
	Repeat	56 – 59	4	3
	Repeat	60 – 63	4	4
	Repeat	64 – 67	4	5
	Repeat	68 – 71	4	6
	Repeat	72 – 75	4	7
	Repeat	76 – 79	4	8
	Repeat	80 – 83	4	9
	Repeat	84 – 87	4	10
	Repeat	88 – 91	4	11
	Repeat	92 – 95	4	12
	Repeat	96 – 99	4	13
	Repeat	100 – 103	4	14
	Repeat	104 – 107	4	15
	Repeat	108 – 111	4	16
	Repeat	112 – 115	4	17
	Repeat	116 – 119	4	18
	Repeat	120 – 123	4	19
	Repeat	124 – 127	4	20
	Repeat	128 – 131	4	21
	Repeat	132 – 135	4	22
	Repeat	136 – 139	4	23
	Repeat	140 – 143	4	24
	Repeat	144 – 147	4	25
	Repeat	148 – 151	4	26
	Repeat	152 – 155	4	27
	Repeat	156 – 159	4	28
	Repeat	160 – 163	4	29
	Repeat	164 – 167	4	30
	Repeat	168 – 171	4	31
	Repeat	172 – 175	4	32
	Repeat	176 – 179	4	33
	Domain	216 – 309	94	PH
	Domain	487 – 622	136	DAGKc
	Zinc finger	327 – 377	51	Phorbol-ester/DAG-type 1
	Zinc finger	398 – 449	52	Phorbol-ester/DAG-type 2
	Region	48 – 179	132	33 X 4 AA approximate tandem repeats of E-P-A-P
	Region	1199 – 1268	70	Required for localization to the plasma membrane
	Motif	1269 – 1271	3	PDZ-binding
Amino acid modifications
	Modified residue	78	1	Phosphotyrosine Ref.1
Natural variations
	Natural variant	1118	1	D → N: dbSNP rs4074320.		VAR_048859
Experimental info
	Mutagenesis	78	1	Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). Ref.1
	Mutagenesis	1075	1	Y → F: Does not affect phosphorylation. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5KSL6-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 1EB8BD12B1992DDB	FASTA	1,271	141,829
	10 20 30 40 50 60 MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS PEPIPEPCPE 70 80 90 100 110 120 LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE PAPEPATEPA PEPAPEPATE 130 140 150 160 170 180 SAPEPTPEPA LESVPEPAPE LTPEVAPELA PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC 190 200 210 220 230 240 LLQCPVDTRE RGLKTSPSPS PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV 250 260 270 280 290 300 QGQKLYFAHH PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW 310 320 330 340 350 360 INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA LSRDAIICEV 370 380 390 400 410 420 CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ WVEGNMPVSS QCAVCHESCG 430 440 450 460 470 480 SYQRLQDFRC LWCNSTVHDD CRRRFSKECC FRSHRSSVIP PTALSDPKGD GQLVVSSDFW 490 500 510 520 530 540 NLDWSSACSC PLLIFINSKS GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA 550 560 570 580 590 600 RFRILVCGGD GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD 610 620 630 640 650 660 ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE SAATELNKIL 670 680 690 700 710 720 KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI VSVILKSDLM YDRLSVLIDV 730 740 750 760 770 780 LAEEAAATSA EKSATEYADS SKADRKPFIP QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV 790 800 810 820 830 840 EQALDEESRQ TISVKNFSST FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL 850 860 870 880 890 900 DHLHFTPESI RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT 910 920 930 940 950 960 KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN TATTEYEAPA 970 980 990 1000 1010 1020 IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI DGEEGIPVQV DGEAWIQRPG 1030 1040 1050 1060 1070 1080 LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH 1090 1100 1110 1120 1130 1140 LARLAENLIS KLNDLSKIHQ HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL 1150 1160 1170 1180 1190 1200 SRNDAVDVTF SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI 1210 1220 1230 1240 1250 1260 FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH RRHREDEAEG 1270 DDPLTPSRSQ L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa." Imai S., Kai M., Yasuda S., Kanoh H., Sakane F. J. Biol. Chem. 280:39870-39881(2005) [PubMed: 16210324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-78, MUTAGENESIS OF TYR-78 AND TYR-1075.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB183864 mRNA. Translation: BAD86792.1. BC137319 mRNA. Translation: AAI37320.1. BC137320 mRNA. Translation: AAI37321.1.
IPI	IPI00046309.
RefSeq	NP_001013764.1.
UniGene	Hs.631770
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q5KSL6.
Proteomic databases
PRIDE	Q5KSL6.
Genome annotation databases
Ensembl	ENST00000376025; ENSP00000365193; ENSG00000204466; Homo sapiens. [Genome view]
GeneID	139189.
KEGG	hsa:139189.
Organism-specific databases
CTD	139189.
GeneCards	GC0XM050128.
H-InvDB	HIX0056304.
HGNC	HGNC:32395. DGKK.
HPA	HPA000523. HPA020458.
PharmGKB	PA142671978.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q5KSL6.
HOVERGEN	Q5KSL6.
Enzyme and pathway databases
BRENDA	2.7.1.107. 247.
Gene expression databases
ArrayExpress	Q5KSL6.
CleanEx	HS_DGKK.
Genevestigator	Q5KSL6.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. [Graphical view]
ProDom	PD002939. DAGKa. 1 hit. PD005043. DAGKc. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	83910.

Entry information

Entry name

DGKK_HUMAN

Accession

Primary (citable) accession number: Q5KSL6
Secondary accession number(s): B2RP91

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	February 15, 2005
Last modified:	October 13, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.