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Protein

Diacylglycerol kinase kappa

Gene

DGKK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Enzyme regulationi

Inhibited in response to H2O2.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri327 – 377Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri398 – 449Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: HGNC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • diacylglycerol metabolic process Source: HGNC
  • intracellular signal transduction Source: HGNC
  • platelet activation Source: Reactome
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  • response to oxidative stress Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-31341-MONOMER.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.

Chemistry databases

SwissLipidsiSLP:000000926.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase kappa (EC:2.7.1.107)
Short name:
DAG kinase kappa
Alternative name(s):
142 kDa diacylglycerol kinase
Diglyceride kinase kappa
Short name:
DGK-kappa
Gene namesi
Name:DGKK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:32395. DGKK.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). 1 Publication1
Mutagenesisi1075Y → F: Does not affect phosphorylation. 1 Publication1

Organism-specific databases

DisGeNETi139189.
OpenTargetsiENSG00000274588.
PharmGKBiPA142671978.

Polymorphism and mutation databases

BioMutaiDGKK.
DMDMi74708075.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393681 – 1271Diacylglycerol kinase kappaAdd BLAST1271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5KSL6.
PeptideAtlasiQ5KSL6.
PRIDEiQ5KSL6.

PTM databases

iPTMnetiQ5KSL6.
PhosphoSitePlusiQ5KSL6.

Expressioni

Tissue specificityi

Expressed in testis, and to a lesser extent in placenta.1 Publication

Gene expression databases

CleanExiHS_DGKK.
GenevisibleiQ5KSL6. HS.

Interactioni

Subunit structurei

Does not form homooligomers.

Structurei

3D structure databases

ProteinModelPortaliQ5KSL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati48 – 5114
Repeati52 – 5524
Repeati56 – 5934
Repeati60 – 6344
Repeati64 – 6754
Repeati68 – 7164
Repeati72 – 7574
Repeati76 – 7984
Repeati80 – 8394
Repeati84 – 87104
Repeati88 – 91114
Repeati92 – 95124
Repeati96 – 99134
Repeati100 – 103144
Repeati104 – 107154
Repeati108 – 111164
Repeati112 – 115174
Repeati116 – 119184
Repeati120 – 123194
Repeati124 – 127204
Repeati128 – 131214
Repeati132 – 135224
Repeati136 – 139234
Repeati140 – 143244
Repeati144 – 147254
Repeati148 – 151264
Repeati152 – 155274
Repeati156 – 159284
Repeati160 – 163294
Repeati164 – 167304
Repeati168 – 171314
Repeati172 – 175324
Repeati176 – 179334
Domaini216 – 309PHPROSITE-ProRule annotationAdd BLAST94
Domaini487 – 622DAGKcPROSITE-ProRule annotationAdd BLAST136

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 17933 X 4 AA approximate tandem repeats of E-P-A-PAdd BLAST132
Regioni1199 – 1268Required for localization to the plasma membraneAdd BLAST70

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1269 – 1271PDZ-binding3

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri327 – 377Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri398 – 449Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00760000119050.
HOVERGENiHBG051346.
InParanoidiQ5KSL6.
KOiK00901.
OMAiENSMKMW.
OrthoDBiEOG091G00SK.
PhylomeDBiQ5KSL6.

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KSL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS
60 70 80 90 100
PEPIPEPCPE LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE
110 120 130 140 150
PAPEPATEPA PEPAPEPATE SAPEPTPEPA LESVPEPAPE LTPEVAPELA
160 170 180 190 200
PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC LLQCPVDTRE RGLKTSPSPS
210 220 230 240 250
PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV QGQKLYFAHH
260 270 280 290 300
PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW
310 320 330 340 350
INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA
360 370 380 390 400
LSRDAIICEV CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ
410 420 430 440 450
WVEGNMPVSS QCAVCHESCG SYQRLQDFRC LWCNSTVHDD CRRRFSKECC
460 470 480 490 500
FRSHRSSVIP PTALSDPKGD GQLVVSSDFW NLDWSSACSC PLLIFINSKS
510 520 530 540 550
GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA RFRILVCGGD
560 570 580 590 600
GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD
610 620 630 640 650
ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE
660 670 680 690 700
SAATELNKIL KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI
710 720 730 740 750
VSVILKSDLM YDRLSVLIDV LAEEAAATSA EKSATEYADS SKADRKPFIP
760 770 780 790 800
QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV EQALDEESRQ TISVKNFSST
810 820 830 840 850
FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL DHLHFTPESI
860 870 880 890 900
RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT
910 920 930 940 950
KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN
960 970 980 990 1000
TATTEYEAPA IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI
1010 1020 1030 1040 1050
DGEEGIPVQV DGEAWIQRPG LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH
1060 1070 1080 1090 1100
TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH LARLAENLIS KLNDLSKIHQ
1110 1120 1130 1140 1150
HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL SRNDAVDVTF
1160 1170 1180 1190 1200
SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI
1210 1220 1230 1240 1250
FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH
1260 1270
RRHREDEAEG DDPLTPSRSQ L
Length:1,271
Mass (Da):141,829
Last modified:February 15, 2005 - v1
Checksum:i1EB8BD12B1992DDB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0488591118D → N.Corresponds to variant rs4074320dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB183864 mRNA. Translation: BAD86792.1.
BC137319 mRNA. Translation: AAI37320.1.
BC137320 mRNA. Translation: AAI37321.1.
CCDSiCCDS75980.1.
RefSeqiNP_001013764.1. NM_001013742.3.
UniGeneiHs.38132.
Hs.631770.

Genome annotation databases

EnsembliENST00000611977; ENSP00000477515; ENSG00000274588.
GeneIDi139189.
KEGGihsa:139189.
UCSCiuc033edr.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB183864 mRNA. Translation: BAD86792.1.
BC137319 mRNA. Translation: AAI37320.1.
BC137320 mRNA. Translation: AAI37321.1.
CCDSiCCDS75980.1.
RefSeqiNP_001013764.1. NM_001013742.3.
UniGeneiHs.38132.
Hs.631770.

3D structure databases

ProteinModelPortaliQ5KSL6.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

SwissLipidsiSLP:000000926.

PTM databases

iPTMnetiQ5KSL6.
PhosphoSitePlusiQ5KSL6.

Polymorphism and mutation databases

BioMutaiDGKK.
DMDMi74708075.

Proteomic databases

MaxQBiQ5KSL6.
PeptideAtlasiQ5KSL6.
PRIDEiQ5KSL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000611977; ENSP00000477515; ENSG00000274588.
GeneIDi139189.
KEGGihsa:139189.
UCSCiuc033edr.2. human.

Organism-specific databases

CTDi139189.
DisGeNETi139189.
GeneCardsiDGKK.
HGNCiHGNC:32395. DGKK.
MIMi300837. gene.
neXtProtiNX_Q5KSL6.
OpenTargetsiENSG00000274588.
PharmGKBiPA142671978.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00760000119050.
HOVERGENiHBG051346.
InParanoidiQ5KSL6.
KOiK00901.
OMAiENSMKMW.
OrthoDBiEOG091G00SK.
PhylomeDBiQ5KSL6.

Enzyme and pathway databases

BioCyciZFISH:G66-31341-MONOMER.
ReactomeiR-HSA-114508. Effects of PIP2 hydrolysis.

Miscellaneous databases

GenomeRNAii139189.
PROiQ5KSL6.
SOURCEiSearch...

Gene expression databases

CleanExiHS_DGKK.
GenevisibleiQ5KSL6. HS.

Family and domain databases

CDDicd00029. C1. 2 hits.
Gene3Di2.30.29.30. 1 hit.
InterProiIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR016064. NAD/diacylglycerol_kinase.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDGKK_HUMAN
AccessioniPrimary (citable) accession number: Q5KSL6
Secondary accession number(s): B2RP91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.