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Q5KSL6 (DGKK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Diacylglycerol kinase kappa
Short name=DAG kinase kappa
EC=2.7.1.107
Alternative name(s):
142 kDa diacylglycerol kinase
Diglyceride kinase kappa
Short name=DGK-kappa
Gene names
Name:DGKK
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length1271 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Ref.1

Catalytic activity

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1

Enzyme regulation

Inhibited in response to H2O2. Ref.1

Subunit structure

Does not form homooligomers.

Subcellular location

Cytoplasm. Membrane; Peripheral membrane protein Ref.1.

Tissue specificity

Expressed in testis, and to a lesser extent in placenta. Ref.1

Post-translational modification

Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2. Ref.1

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 1 PH domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12711271Diacylglycerol kinase kappa
PRO_0000239368

Regions

Repeat48 – 5141
Repeat52 – 5542
Repeat56 – 5943
Repeat60 – 6344
Repeat64 – 6745
Repeat68 – 7146
Repeat72 – 7547
Repeat76 – 7948
Repeat80 – 8349
Repeat84 – 87410
Repeat88 – 91411
Repeat92 – 95412
Repeat96 – 99413
Repeat100 – 103414
Repeat104 – 107415
Repeat108 – 111416
Repeat112 – 115417
Repeat116 – 119418
Repeat120 – 123419
Repeat124 – 127420
Repeat128 – 131421
Repeat132 – 135422
Repeat136 – 139423
Repeat140 – 143424
Repeat144 – 147425
Repeat148 – 151426
Repeat152 – 155427
Repeat156 – 159428
Repeat160 – 163429
Repeat164 – 167430
Repeat168 – 171431
Repeat172 – 175432
Repeat176 – 179433
Domain216 – 30994PH
Domain487 – 622136DAGKc
Zinc finger327 – 37751Phorbol-ester/DAG-type 1
Zinc finger398 – 44952Phorbol-ester/DAG-type 2
Region48 – 17913233 X 4 AA approximate tandem repeats of E-P-A-P
Region1199 – 126870Required for localization to the plasma membrane
Motif1269 – 12713PDZ-binding

Amino acid modifications

Modified residue781Phosphotyrosine Ref.1

Natural variations

Natural variant11181D → N.
Corresponds to variant rs4074320 [ dbSNP | Ensembl ].
VAR_048859

Experimental info

Mutagenesis781Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). Ref.1
Mutagenesis10751Y → F: Does not affect phosphorylation. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5KSL6 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 1EB8BD12B1992DDB

FASTA1,271141,829
        10         20         30         40         50         60 
MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS PEPIPEPCPE 

        70         80         90        100        110        120 
LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE PAPEPATEPA PEPAPEPATE 

       130        140        150        160        170        180 
SAPEPTPEPA LESVPEPAPE LTPEVAPELA PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC 

       190        200        210        220        230        240 
LLQCPVDTRE RGLKTSPSPS PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV 

       250        260        270        280        290        300 
QGQKLYFAHH PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW 

       310        320        330        340        350        360 
INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA LSRDAIICEV 

       370        380        390        400        410        420 
CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ WVEGNMPVSS QCAVCHESCG 

       430        440        450        460        470        480 
SYQRLQDFRC LWCNSTVHDD CRRRFSKECC FRSHRSSVIP PTALSDPKGD GQLVVSSDFW 

       490        500        510        520        530        540 
NLDWSSACSC PLLIFINSKS GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA 

       550        560        570        580        590        600 
RFRILVCGGD GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD 

       610        620        630        640        650        660 
ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE SAATELNKIL 

       670        680        690        700        710        720 
KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI VSVILKSDLM YDRLSVLIDV 

       730        740        750        760        770        780 
LAEEAAATSA EKSATEYADS SKADRKPFIP QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV 

       790        800        810        820        830        840 
EQALDEESRQ TISVKNFSST FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL 

       850        860        870        880        890        900 
DHLHFTPESI RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT 

       910        920        930        940        950        960 
KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN TATTEYEAPA 

       970        980        990       1000       1010       1020 
IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI DGEEGIPVQV DGEAWIQRPG 

      1030       1040       1050       1060       1070       1080 
LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH 

      1090       1100       1110       1120       1130       1140 
LARLAENLIS KLNDLSKIHQ HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL 

      1150       1160       1170       1180       1190       1200 
SRNDAVDVTF SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI 

      1210       1220       1230       1240       1250       1260 
FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH RRHREDEAEG 

      1270 
DDPLTPSRSQ L

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa."
Imai S., Kai M., Yasuda S., Kanoh H., Sakane F.
J. Biol. Chem. 280:39870-39881(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-78, MUTAGENESIS OF TYR-78 AND TYR-1075.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB183864 mRNA. Translation: BAD86792.1.
BC137319 mRNA. Translation: AAI37320.1.
BC137320 mRNA. Translation: AAI37321.1.
IPIIPI00046309.
RefSeqNP_001013764.1. NM_001013742.2.
UniGeneHs.631770.

3D structure databases

ProteinModelPortalQ5KSL6.
ModBaseSearch...

PTM databases

PhosphoSiteQ5KSL6.

Polymorphism databases

DMDM74708075.

Proteomic databases

PRIDEQ5KSL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID139189.
KEGGhsa:139189.
UCSCuc010njr.2. human.

Organism-specific databases

CTD139189.
GeneCardsGC0XM050128.
HGNCHGNC:32395. DGKK.
MIM300837. gene.
neXtProtNX_Q5KSL6.
PharmGKBPA142671978.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG051346.
InParanoidQ5KSL6.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

CleanExHS_DGKK.
GenevestigatorQ5KSL6.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
PROSITEPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi139189.
NextBio83910.
SOURCESearch...

Entry information

Entry nameDGKK_HUMAN
AccessionPrimary (citable) accession number: Q5KSL6
Secondary accession number(s): B2RP91
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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