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Q5KSL6

- DGKK_HUMAN

UniProt

Q5KSL6 - DGKK_HUMAN

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Protein

Diacylglycerol kinase kappa

Gene
DGKK
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Enzyme regulationi

Inhibited in response to H2O2.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri327 – 37751Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri398 – 44952Phorbol-ester/DAG-type 2Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: HGNC
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. diacylglycerol metabolic process Source: HGNC
  3. intracellular signal transduction Source: HGNC
  4. platelet activation Source: Reactome
  5. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  6. response to oxidative stress Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_2202. Effects of PIP2 hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase kappa (EC:2.7.1.107)
Short name:
DAG kinase kappa
Alternative name(s):
142 kDa diacylglycerol kinase
Diglyceride kinase kappa
Short name:
DGK-kappa
Gene namesi
Name:DGKK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:32395. DGKK.

Subcellular locationi

Cytoplasm. Membrane; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi78 – 781Y → F: Induces a strong reduction in phosphorylation but is still sensitive to H(2)O(2). 1 Publication
Mutagenesisi1075 – 10751Y → F: Does not affect phosphorylation. 1 Publication

Organism-specific databases

PharmGKBiPA142671978.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12711271Diacylglycerol kinase kappaPRO_0000239368Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5KSL6.
PRIDEiQ5KSL6.

PTM databases

PhosphoSiteiQ5KSL6.

Expressioni

Tissue specificityi

Expressed in testis, and to a lesser extent in placenta.1 Publication

Gene expression databases

CleanExiHS_DGKK.
GenevestigatoriQ5KSL6.

Interactioni

Subunit structurei

Does not form homooligomers.

Structurei

3D structure databases

ProteinModelPortaliQ5KSL6.
SMRiQ5KSL6. Positions 383-449.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati48 – 5141
Repeati52 – 5542
Repeati56 – 5943
Repeati60 – 6344
Repeati64 – 6745
Repeati68 – 7146
Repeati72 – 7547
Repeati76 – 7948
Repeati80 – 8349
Repeati84 – 87410
Repeati88 – 91411
Repeati92 – 95412
Repeati96 – 99413
Repeati100 – 103414
Repeati104 – 107415
Repeati108 – 111416
Repeati112 – 115417
Repeati116 – 119418
Repeati120 – 123419
Repeati124 – 127420
Repeati128 – 131421
Repeati132 – 135422
Repeati136 – 139423
Repeati140 – 143424
Repeati144 – 147425
Repeati148 – 151426
Repeati152 – 155427
Repeati156 – 159428
Repeati160 – 163429
Repeati164 – 167430
Repeati168 – 171431
Repeati172 – 175432
Repeati176 – 179433
Domaini216 – 30994PHAdd
BLAST
Domaini487 – 622136DAGKcAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 17913233 X 4 AA approximate tandem repeats of E-P-A-PAdd
BLAST
Regioni1199 – 126870Required for localization to the plasma membraneAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1269 – 12713PDZ-binding

Sequence similaritiesi

Contains 1 DAGKc domain.
Contains 1 PH domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri327 – 37751Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri398 – 44952Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG051346.
InParanoidiQ5KSL6.
OMAiGSVQMAM.
PhylomeDBiQ5KSL6.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5KSL6-1 [UniParc]FASTAAdd to Basket

« Hide

MDRGAAAAQG TAPPQDGEQP AESPEPPPPW PPPPPPPAPP PAPPLLSEAS     50
PEPIPEPCPE LAPGPCPEAT SESATELYTE PTPEPATEPA SEPAPEPATE 100
PAPEPATEPA PEPAPEPATE SAPEPTPEPA LESVPEPAPE LTPEVAPELA 150
PEPTPEPVTE LAPEFCPEAA PEFRPSPAPC LLQCPVDTRE RGLKTSPSPS 200
PSPSPRTPMS WSRIKKILKE GPMLKNCNSF KRWKLRYFLV QGQKLYFAHH 250
PAFAHFETID LSQATVAESS CRNLCHSFCV ITPQRKITLA APNRKDMEEW 300
INIIKTIQQG EIYKIPAAEN NPFLVGMHCW YSSYSHRTQH CNVCRESIPA 350
LSRDAIICEV CKVKSHRLCA LRASKDCKWN TLSITDDLLL PADEVNMPHQ 400
WVEGNMPVSS QCAVCHESCG SYQRLQDFRC LWCNSTVHDD CRRRFSKECC 450
FRSHRSSVIP PTALSDPKGD GQLVVSSDFW NLDWSSACSC PLLIFINSKS 500
GDHQGIVFLR KFKQYLNPSQ VFDLLKGGPE AGLSMFKNFA RFRILVCGGD 550
GSVSWVLSLI DAFGLHEKCQ LAVIPLGTGN DLARVLGWGA FWNKSKSPLD 600
ILNRVEQASV RILDRWSVMI RETPRQTPLL KGQVEMDVPR FEAAAIQHLE 650
SAATELNKIL KAKYPTEMII ATRFLCSAVE DFVVDIVKAW GQIKQNNTAI 700
VSVILKSDLM YDRLSVLIDV LAEEAAATSA EKSATEYADS SKADRKPFIP 750
QIDHIAKCKL ELATKAQSLQ KSLKLIIFQV EQALDEESRQ TISVKNFSST 800
FFLEDDPEDI NQTSPRRRSR RGTLSSISSL KSEDLDNLNL DHLHFTPESI 850
RFKEKCVMNN YFGIGLDAKI SLDFNTRRDE HPGQYNSRLK NKMWYGLLGT 900
KELLQRSYRK LEERVHLECD GETISLPNLQ GIVVLNITSY AGGINFWGSN 950
TATTEYEAPA IDDGKLEVVA IFGSVQMAMS RIINLHHHRI AQCHEVMITI 1000
DGEEGIPVQV DGEAWIQRPG LIKIRYKNAA QMLTRDRDFE NSMKMWEYKH 1050
TEIQAAPQPQ LDFQDSQESL SDEEYAQMQH LARLAENLIS KLNDLSKIHQ 1100
HVSVLMGSVN ASANILNDIF YGQDSGNEMG AASCIPIETL SRNDAVDVTF 1150
SLKGLYDDTT AFLDEKLLRS AEDETALQSA LDAMNKEFKK LSEIDWMNPI 1200
FVPEEKSSDT DSRSLRLKIK FPKLGKKKVE EERKPKSGQS VQSFIGNLWH 1250
RRHREDEAEG DDPLTPSRSQ L 1271
Length:1,271
Mass (Da):141,829
Last modified:February 15, 2005 - v1
Checksum:i1EB8BD12B1992DDB
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1118 – 11181D → N.
Corresponds to variant rs4074320 [ dbSNP | Ensembl ].
VAR_048859

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB183864 mRNA. Translation: BAD86792.1.
BC137319 mRNA. Translation: AAI37320.1.
BC137320 mRNA. Translation: AAI37321.1.
RefSeqiNP_001013764.1. NM_001013742.3.
UniGeneiHs.38132.
Hs.631770.

Genome annotation databases

GeneIDi139189.
KEGGihsa:139189.
UCSCiuc010njr.2. human.

Polymorphism databases

DMDMi74708075.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB183864 mRNA. Translation: BAD86792.1 .
BC137319 mRNA. Translation: AAI37320.1 .
BC137320 mRNA. Translation: AAI37321.1 .
RefSeqi NP_001013764.1. NM_001013742.3.
UniGenei Hs.38132.
Hs.631770.

3D structure databases

ProteinModelPortali Q5KSL6.
SMRi Q5KSL6. Positions 383-449.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q5KSL6.

Polymorphism databases

DMDMi 74708075.

Proteomic databases

MaxQBi Q5KSL6.
PRIDEi Q5KSL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 139189.
KEGGi hsa:139189.
UCSCi uc010njr.2. human.

Organism-specific databases

CTDi 139189.
GeneCardsi GC0XM050128.
HGNCi HGNC:32395. DGKK.
MIMi 300837. gene.
neXtProti NX_Q5KSL6.
PharmGKBi PA142671978.
GenAtlasi Search...

Phylogenomic databases

HOVERGENi HBG051346.
InParanoidi Q5KSL6.
OMAi GSVQMAM.
PhylomeDBi Q5KSL6.

Enzyme and pathway databases

Reactomei REACT_2202. Effects of PIP2 hydrolysis.

Miscellaneous databases

GenomeRNAii 139189.
NextBioi 83910.
PROi Q5KSL6.
SOURCEi Search...

Gene expression databases

CleanExi HS_DGKK.
Genevestigatori Q5KSL6.

Family and domain databases

Gene3Di 2.30.29.30. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa."
    Imai S., Kai M., Yasuda S., Kanoh H., Sakane F.
    J. Biol. Chem. 280:39870-39881(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT TYR-78, MUTAGENESIS OF TYR-78 AND TYR-1075.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiDGKK_HUMAN
AccessioniPrimary (citable) accession number: Q5KSL6
Secondary accession number(s): B2RP91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: February 15, 2005
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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