Q5KR47 (TPM3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin alpha-3 chain

Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3

Gene names

Name:

TPM3

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments By similarity.
Subunit structure	Heterodimer of an alpha and a beta chain. Binds to TMOD1 By similarity.
Subcellular location	Isoform 2: Cytoplasm › cytoskeleton By similarity.
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Muscle protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW stress fiber Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5KR47-1)

Also known as: Skeletal muscle;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5KR47-2)

Also known as: Cytoskeletal;

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MEAIKKKMQM...LELAEKKAAD → MAGITTIEAV...VEGERRAREQ
     189-211: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

Note: Initiator Met-1 is removed (By similarity). Contains a N-acetylalanine at position 2. Contains a N6-acetyllysine at position 228 (By similarity).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tropomyosin alpha-3 chain		PRO_0000289991
Regions
	Coiled coil	1 – 284	284	By similarity
Amino acid modifications
	Modified residue	283	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 80	80	MEAIK…KKAAD → MAGITTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2.		VSP_026081
	Alternative sequence	189 – 211	23	KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2.		VSP_026082
	Alternative sequence	259 – 284	26	ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2.		VSP_026083

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Skeletal muscle) [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A3BCE715565DABBD	FASTA	284	32,819
	10 20 30 40 50 60 MEAIKKKMQM LKLDKENALD RAEQAEAEQK QAEERSKQLE DELAAMQKKL KGTEDELDKY 70 80 90 100 110 120 SEALKDAQEK LELAEKKAAD AEAEVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ENRALKDEEK MELQEIQLKE AKHIAEEADR KYEEVARKLV IIEGDLERTE 190 200 210 220 230 240 ERAELAESKC SELEEELKNV TNNLKSLEAQ AEKYSQKEDK YEEEIKILTD KLKEAETRAE 250 260 270 280 FAERSVAKLE KTIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
	Isoform 2 (Cytoskeletal) [UniParc]. Checksum: 5C049312A337BC19	FASTA	248	29,033
	10 20 30 40 50 60 MAGITTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA SLNRRIQLVE 70 80 90 100 110 120 EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRALK DEEKMELQEI QLKEAKHIAE 130 140 150 160 170 180 EADRKYEEVA RKLVIIEGDL ERTEERAELA ESRCREMDEQ IRLMDQNLKC LSAAEEKYSQ 190 200 210 220 230 240 KEDKYEEEIK ILTDKLKEAE TRAEFAERSV AKLEKTIDDL EDKLKCTKEE HLCTQRMLDQ TLLDLNEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bovine tropomyosin isoforms." Oe M., Nakajima I., Muroya S., Chikuni K. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Skeletal muscle.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB198072 mRNA. Translation: BAD86592.1. BC108106 mRNA. Translation: AAI08107.1.
IPI	IPI00689865. IPI00714405.
RefSeq	NP_001011674.1. NM_001011674.1.
UniGene	Bt.55987.
3D structure databases
HSSP	HSSP built from PDB template 1C1G based on UniProtKB P42639.
ProteinModelPortal	Q5KR47.
ModBase	Search...
Proteomic databases
PaxDb	Q5KR47.
PRIDE	Q5KR47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000013077; ENSBTAP00000013077; ENSBTAG00000033217. ENSBTAT00000047197; ENSBTAP00000044420; ENSBTAG00000033217.
GeneID	497019.
KEGG	bta:497019.
Organism-specific databases
CTD	7170.
Phylogenomic databases
eggNOG	NOG304012.
GeneTree	ENSGT00550000074494.
HOGENOM	HOG000231521.
HOVERGEN	HBG107404.
InParanoid	Q5KR47.
KO	K09290.
OMA	ADESECM.
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20865832.

Entry information

Entry name

TPM3_BOVIN

Accession

Primary (citable) accession number: Q5KR47
Secondary accession number(s): Q32PI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program