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Protein

Tropomyosin alpha-3 chain

Gene

TPM3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments (By similarity).By similarity

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.
R-BTA-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-3 chain
Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3
Gene namesi
Name:TPM3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

Isoform 2 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002899911 – 284Tropomyosin alpha-3 chainAdd BLAST284
Isoform 2 (identifier: Q5KR47-2)
Initiator methionineiRemoved By similarityBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei87PhosphoserineBy similarity1
Modified residuei108PhosphothreonineBy similarity1
Modified residuei206PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei252PhosphothreonineBy similarity1
Modified residuei261PhosphotyrosineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei282PhosphothreonineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Isoform 2 (identifier: Q5KR47-2)
Modified residuei2N-acetylalanineBy similarity1
Modified residuei228N6-acetyllysine By similarityBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5KR47.
PeptideAtlasiQ5KR47.
PRIDEiQ5KR47.

Expressioni

Gene expression databases

BgeeiENSBTAG00000033217.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain. Binds to TMOD1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013077.

Structurei

3D structure databases

ProteinModelPortaliQ5KR47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 284By similarityAdd BLAST284

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiQ5KR47.
KOiK09290.
OMAiXLEDELA.
OrthoDBiEOG091G0UO7.
TreeFamiTF351519.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5KR47-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAIKKKMQM LKLDKENALD RAEQAEAEQK QAEERSKQLE DELAAMQKKL
60 70 80 90 100
KGTEDELDKY SEALKDAQEK LELAEKKAAD AEAEVASLNR RIQLVEEELD
110 120 130 140 150
RAQERLATAL QKLEEAEKAA DESERGMKVI ENRALKDEEK MELQEIQLKE
160 170 180 190 200
AKHIAEEADR KYEEVARKLV IIEGDLERTE ERAELAESKC SELEEELKNV
210 220 230 240 250
TNNLKSLEAQ AEKYSQKEDK YEEEIKILTD KLKEAETRAE FAERSVAKLE
260 270 280
KTIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
Length:284
Mass (Da):32,819
Last modified:February 15, 2005 - v1
Checksum:iA3BCE715565DABBD
GO
Isoform 2 (identifier: Q5KR47-2) [UniParc]FASTAAdd to basket
Also known as: Cytoskeletal

The sequence of this isoform differs from the canonical sequence as follows:
     1-80: MEAIKKKMQM...LELAEKKAAD → MAGITTIEAV...VEGERRAREQ
     189-211: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     259-284: ELYAQKLKYKAISEELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

Show »
Length:248
Mass (Da):29,033
Checksum:i5C049312A337BC19
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0260811 – 80MEAIK…KKAAD → MAGITTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_026082189 – 211KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_026083259 – 284ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB198072 mRNA. Translation: BAD86592.1.
BC108106 mRNA. Translation: AAI08107.1.
RefSeqiNP_001011674.1. NM_001011674.1. [Q5KR47-1]
XP_005203687.1. XM_005203630.3. [Q5KR47-1]
XP_005203696.1. XM_005203639.3. [Q5KR47-2]
UniGeneiBt.55987.

Genome annotation databases

EnsembliENSBTAT00000013077; ENSBTAP00000013077; ENSBTAG00000033217. [Q5KR47-1]
ENSBTAT00000047197; ENSBTAP00000044420; ENSBTAG00000033217. [Q5KR47-2]
GeneIDi497019.
KEGGibta:497019.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB198072 mRNA. Translation: BAD86592.1.
BC108106 mRNA. Translation: AAI08107.1.
RefSeqiNP_001011674.1. NM_001011674.1. [Q5KR47-1]
XP_005203687.1. XM_005203630.3. [Q5KR47-1]
XP_005203696.1. XM_005203639.3. [Q5KR47-2]
UniGeneiBt.55987.

3D structure databases

ProteinModelPortaliQ5KR47.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000013077.

Proteomic databases

PaxDbiQ5KR47.
PeptideAtlasiQ5KR47.
PRIDEiQ5KR47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000013077; ENSBTAP00000013077; ENSBTAG00000033217. [Q5KR47-1]
ENSBTAT00000047197; ENSBTAP00000044420; ENSBTAG00000033217. [Q5KR47-2]
GeneIDi497019.
KEGGibta:497019.

Organism-specific databases

CTDi7170.

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiQ5KR47.
KOiK09290.
OMAiXLEDELA.
OrthoDBiEOG091G0UO7.
TreeFamiTF351519.

Enzyme and pathway databases

ReactomeiR-BTA-390522. Striated Muscle Contraction.
R-BTA-445355. Smooth Muscle Contraction.

Gene expression databases

BgeeiENSBTAG00000033217.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPM3_BOVIN
AccessioniPrimary (citable) accession number: Q5KR47
Secondary accession number(s): Q32PI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: February 15, 2005
Last modified: October 5, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.