Reviewed, UniProtKB/Swiss-Prot Q5KQT6 (CP1A2_FELCA)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A2
    EC=1.14.14.1
Alternative name(s):
    CYPIA2

Gene names

Name:

CYP1A2

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Microsome membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Cytochrome P450 1A2		PRO_0000226727
Sites
	Metal binding	454	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	285	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KQT6-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 03C59CEC7676D7DF	FASTA	512	57,662
	10 20 30 40 50 60 MAISPTATEL LLASFAFCLV FWVVRAWQPR VPKGLKSPPG PWGWPLLGHV LTLGKNPHLV 70 80 90 100 110 120 LARLSQRYGD VLQIRIGSTP VLVLSGLDTI RQALVQQGDD FKGRPNLYSF SLVTDGHSMS 130 140 150 160 170 180 FSPDSGPVWA ARRRLAQSAL NTFSIASDPA SSSSCYLEDH VSKEAEALLS RLQEQMAEVG 190 200 210 220 230 240 SFDPHSQVVL SVANVIGAMC FGQHFPQDSE EKLSLIHSSN IFVENAYSGN PVDFFPILQY 250 260 270 280 290 300 IPTPGLQRFK AFNQKLVQFL QKIIQEHYQD FDENNIQDIT GALLKHCKKG SRANGGRIPH 310 320 330 340 350 360 EKIVSLINDI FGAGFDTVTT AISWSLMYLV TNPEKQRKIQ EELDTVVGRA RRPRLSDRLQ 370 380 390 400 410 420 LPYLEASILE IFRHSSFIPF TVPHSTTRDT TLNGFYIPEK HLVFINQWQV NHDQKVWGDP 430 440 450 460 470 480 FEFRPERFLT ADGTAINKIL SEKVMIFGLG KRRCIGEVLA KWEVFLFLAI LLQQLEFSVP 490 500 510 AGVKVDLTPI YGLTMRHVRC EHVQARPRFS IK

References

[1]	"Feline cytochrome P450 1A2." Tanaka N., Yokota H. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB199731 mRNA. Translation: BAD86853.1.
RefSeq	NP_001041478.1.
3D structure databases
SMR	Q5KQT6. Positions 30-510.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KQT6.
Genome annotation databases
Ensembl	ENSFCAT00000000343; ENSFCAP00000000315; ENSFCAG00000000344; Felis catus. [Genome view]
GeneID	554345.
Organism-specific databases
CTD	554345.
Phylogenomic databases
HOVERGEN	Q5KQT6.
Enzyme and pathway databases
BRENDA	1.14.14.1. 273949.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A2_FELCA

Accession

Primary (citable) accession number: Q5KQT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	February 15, 2005
Last modified:	October 13, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)