Reviewed, UniProtKB/Swiss-Prot Q5KP87 (GLGB_CRYNE)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme

Gene names

Name:	GLC3
Ordered Locus Names:	CNA03810, CNBA3620

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 682	682	1,4-alpha-glucan-branching enzyme		PRO_0000188780
Sites
	Active site	236	1	By similarity
	Active site	271	1	By similarity
	Active site	276	1	By similarity
	Active site	340	1	By similarity
	Active site	342	1	By similarity
	Active site	397	1	By similarity
	Active site	465	1	By similarity
	Active site	466	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KP87-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: AD1D30BE01EB496F	FASTA	682	78,344
	10 20 30 40 50 60 MTAVSLSDGT AVLKTDPWLE PFSGALRERY AAYQKQRTII EEHEGGLAEF SKGYKSMGFQ 70 80 90 100 110 120 IDKNGGVRYR EWASNATEAR LIGEFNNWSH TANPMTKSPF GVWECYVPPV SPGVCAIPHD 130 140 150 160 170 180 SMVKISMTLP GGESIDRIPT WITRVTQDLN ISPIYDGRFW NPPKEQQYQF KHGHSTRPVE 190 200 210 220 230 240 GLKIYEAHVG ISSPNMRVTT YKEFEVDVLP KIKQLGYNCI QMMAIMEHAY YASFGYQVTN 250 260 270 280 290 300 FFAASSRFGT PEELKSLVDK AHELGLTVLL DVVHSHASKN ILDGINMYDG SDHLYFHEGG 310 320 330 340 350 360 RGRHDQWDSR LFNYGQHEVL RFLLSNLRFW MDIYMFDGFR FDGVTSMMYK HHGIGSGFSG 370 380 390 400 410 420 GYHEYFGDSV DLEAMVYLML ANAMLHETYP HVVTIAEDVS GMPTLCRPVA EGGVGFDYRL 430 440 450 460 470 480 SMAIPDMWIK LLKEYTDDQW EMGQIVHNLT NRRHLEKSVA YAESHDQALV GDKTLAFWLM 490 500 510 520 530 540 DKEMYDFMSD LSPLTPIIDR GLALHKMIRF IVHTLGGEAY LNFEGNEFGH PEWMDFPREG 550 560 570 580 590 600 NGNSFAHARR QFNLVDDKLL RYKYLYEFDV AMNWLEDKYK WLNSPQAYVS LKHEGDKMIV 610 620 630 640 650 660 FERAGLLFIF NFHPTQSFTD YRVGVDTAGE YKVILTSDET RFGGHNRIDM GGRYFTTPME 670 680 WNGRKNWLQV YSPSRTVLVL GL

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017341 Genomic DNA. Translation: AAW40900.1. AAEY01000002 Genomic DNA. Translation: EAL23246.1.
RefSeq	XP_566719.1. XP_777893.1.
UniGene	Fne.7311
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KP87.
Genome annotation databases
GeneID	3253422. 4933620.
GenomeReviews	Gene locus CNA03810 in contig AE017341_GR.
KEGG	cnb:CNBA3620. cne:CNA03810.
Phylogenomic databases
eggNOG	fuNOG05596.
HOGENOM	HBG302308.
OMA	WNMGDIV.
OrthoDB	EOG9MCZGQ.
PhylomeDB	Q5KP87.
Enzyme and pathway databases
BRENDA	2.4.1.18. 2772.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLGB_CRYNE

Accession

Primary (citable) accession number: Q5KP87
Secondary accession number(s): Q55ZX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)