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Q5KP44

- IMDH_CRYNJ

UniProt

Q5KP44 - IMDH_CRYNJ

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

CNA04240

Organism
Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi340 – 3401Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi342 – 3421Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei343 – 3431IMPUniRule annotation
    Active sitei345 – 3451Thioimidate intermediateUniRule annotation
    Metal bindingi345 – 3451Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei470 – 4701IMPUniRule annotation
    Metal bindingi529 – 5291Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi530 – 5301Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi531 – 5311Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi288 – 2903NADUniRule annotation
    Nucleotide bindingi338 – 3403NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Ordered Locus Names:CNA04240
    OrganismiCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
    Taxonomic identifieri214684 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000002149: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 544544Inosine-5'-monophosphate dehydrogenasePRO_0000415685Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi214684.CNA04240.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5KP44.
    SMRiQ5KP44. Positions 28-129, 242-543.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini132 – 19261CBS 1UniRule annotationAdd
    BLAST
    Domaini194 – 25057CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni378 – 3803IMP bindingUniRule annotation
    Regioni401 – 4022IMP bindingUniRule annotation
    Regioni425 – 4295IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    HOGENOMiHOG000165752.
    KOiK00088.
    OrthoDBiEOG793BHK.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5KP44-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADTNPNAPP RSDSLLNPAD ALKYLEEYPR GDGLSLQELM DSRKNGGLTY    50
    NDFLVLPGHI SFPASDVSLQ SRATKNIVLN TPFLSSPMDT VTEDRMAIAL 100
    ALHGGLGIIH HNCSAEEQAA MVRRVKKYEN GFITDPLCLG PDATVGDVLE 150
    IKAKFGFCGV PITETGAPNS KLLGIVTGRD VQFQDAETPI KSVMTTEVVT 200
    GSSPITLEKA NSLLRETKKG KLPIVDSNGH LVSLVARSDL LKNQNYPYAS 250
    KVPESKQLYC GAAIGTRPGD KDRLKLLAEA GLDVVVLDSS QGDSVYQIEF 300
    IKWIKQTYPK IEIIAGNVVT REQAAQLIAA GADGLRIGMG SGSICITQEV 350
    MAVGRPQGTA VYAVAEFASR FGIPCIADGG IGNIGHIAKA LALGASAVMM 400
    GGLLAGTTES PGEYFYHEGK RVKVYRGMGS IEAMEHTQRG SASGKRSILN 450
    LDNAATARYF SEADAVKVAQ GVSGDVADKG SINKFVPYLF TGLQHSFQDA 500
    GVKSVSELHS CARSGSLRFE LRTASAQLEG GVHGLNSYTK RLFA 544
    Length:544
    Mass (Da):57,899
    Last modified:February 15, 2005 - v1
    Checksum:iE5AB349D78F302AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017341 Genomic DNA. Translation: AAW40949.1.
    RefSeqiXP_566768.1. XM_566768.1.
    UniGeneiFne.7688.

    Genome annotation databases

    EnsemblFungiiAAW40949; AAW40949; CNA04240.
    GeneIDi3253376.
    KEGGicne:CNA04240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017341 Genomic DNA. Translation: AAW40949.1 .
    RefSeqi XP_566768.1. XM_566768.1.
    UniGenei Fne.7688.

    3D structure databases

    ProteinModelPortali Q5KP44.
    SMRi Q5KP44. Positions 28-129, 242-543.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 214684.CNA04240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii AAW40949 ; AAW40949 ; CNA04240 .
    GeneIDi 3253376.
    KEGGi cne:CNA04240.

    Phylogenomic databases

    HOGENOMi HOG000165752.
    KOi K00088.
    OrthoDBi EOG793BHK.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans."
      Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S.
      , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.
      Science 307:1321-1324(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JEC21 / ATCC MYA-565.

    Entry informationi

    Entry nameiIMDH_CRYNJ
    AccessioniPrimary (citable) accession number: Q5KP44
    Secondary accession number(s): Q55ZT3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3