Reviewed, UniProtKB/Swiss-Prot Q5KM33 (ESA1_CRYNE)

Names and origin

Protein names

Recommended name:
    Histone acetyltransferase ESA1
    EC=2.3.1.48

Gene names

Name:	ESA1
Ordered Locus Names:	CNB03160, CNBB2530

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	564 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic component of the NuA4 histone acetyltransferase (HAT) complex which is involved in epigenetic transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4, H3, H2B, H2A and H2A variant H2A.Z. Acetylates histone H4 to form H4K5ac, H4K8ac, H4K12ac and H4K16ac, histone H3 to form H3K14ac, and histone H2A to form H2AK4ac and H2AK7ac. Acetylation of histone H4 is essential for DNA double-strand break repair through homologous recombination. Involved in cell cycle progression. Recruitment to promoters depends on H3K4me By similarity.
Catalytic activity	Acetyl-CoA + histone = CoA + acetylhistone.
Subunit structure	Component of the NuA4 histone acetyltransferase complex By similarity.
Subcellular location	Nucleus By similarity.
Domain	The ESA1-RPD3 motif is common to ESA1 and RPD3 and is required for ESA1 histone acetyl-transferase (HAT) activity and RPD3 histone deacetylase (HDAC) activity.
Sequence similarities	Belongs to the MYST (SAS/MOZ) family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Chromatin regulator Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromatin Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chromatin binding Inferred from electronic annotation. Source: InterPro histone acetyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 564	564	Histone acetyltransferase ESA1		PRO_0000051555
Regions
	Motif	364 – 385	22	ESA1-RPD3 motif By similarity
	Compositional bias	65 – 85	21	Pro-rich
	Compositional bias	121 – 199	79	Lys-rich
Sites
	Active site	423	1	By similarity
	Binding site	426	1	Coenzyme A By similarity
	Binding site	461	1	Coenzyme A By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5KM33-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 76BE79DB995BF692	FASTA	564	63,050
	10 20 30 40 50 60 MSPSAPSTPH GRGSGSEPGT PAPSVAAGGS YTIDDVVPGV KIYVIKPLSN GQAEQRRAEI 70 80 90 100 110 120 LSTRPKPKPS AFAPPPPPNA PSPDPRDDTE YYVHYVEFNK RLDEWVGGSR LVLSKEMEWP 130 140 150 160 170 180 KSKDEPKKKD RPAKAQPSKA PSRATGSPIP SDSLLKKAAN KAAMAAGKAT PGKAMPSSKL 190 200 210 220 230 240 GKASKIGKAG KFPQKRKAKT EADTEAEEES NEDNDALGEE EDMDEDGDVT LITSDGAIDP 250 260 270 280 290 300 SREVVAAPSN PRAAPQVFSK KQEIEKLRTS GSMTQSHSEI SRVKNLNKLQ IGKHEVETWY 310 320 330 340 350 360 FSPYPIEYAH LPVLYICEFC LLYYPSATQL RRHRAKCTLL HPPGNEIYRH EGISFFEIDG 370 380 390 400 410 420 RKQRTWCRNL CLISKCFLDH KTLYYDVDPF LYYCMTVKDD YGCHLIGYFS KEKESAEGYN 430 440 450 460 470 480 VACILTLPQH QRKGYGRLLI EFSYELSKVE GKLGSPEKPL SDLGLLGYRA YWQEKIVELL 490 500 510 520 530 540 LDSDYEISLD EIAQKTSITH GDIMHTCQAL QMIKYYKNSH IIHLTDAVIE QHKKTKAKPR 550 560 RAINPAYLKW KPPVFSRAQL AFGF

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017342 Genomic DNA. Translation: AAW41586.1. AAEY01000009 Genomic DNA. Translation: EAL22621.1.
RefSeq	XP_568893.1. XP_777268.1.
UniGene	Fne.2644 Fne.5908
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KM33.
Genome annotation databases
GeneID	3255895. 4934345.
GenomeReviews	Gene locus CNB03160 in contig AE017342_GR. Gene locus CNBB2530 in contig CM000041_GR.
KEGG	cnb:CNBB2530. cne:CNB03160.
Phylogenomic databases
HOGENOM	Q5KM33.
OMA	DPFLYYC.
Enzyme and pathway databases
BRENDA	2.3.1.48. 2772.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000953. Chromodomain. IPR002717. MOZ_SAS. IPR011991. Wing_hlx_DNA_bd. IPR015880. Znf_C2H2-like. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01853. MOZ_SAS. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. SM00355. ZnF_C2H2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ESA1_CRYNE

Accession

Primary (citable) accession number: Q5KM33
Secondary accession number(s): Q55XW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)