Reviewed, UniProtKB/Swiss-Prot Q5KLF2 (ALG13_CRYNE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine transferase subunit ALG13
    EC=2.4.1.141
Alternative name(s):
    Asparagine-linked glycosylation protein 13

Gene names

Name:	ALG13
Ordered Locus Names:	CNB05490, CNBB0300

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-diphosphodolichol = UDP + N,N'-diacetylchitobiosyl-diphosphodolichol.
Subunit structure	Heterodimer with ALG14 to form a functional enzyme By similarity.
Subcellular location	Endoplasmic reticulum By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family.
Sequence caution	The sequence AAW41985.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	UDP-N-acetylglucosamine transferase subunit ALG13		PRO_0000215600

Sequences

	Sequence		Length	Mass (Da)
	Q5KLF2-1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: C24A865CF7DEA59B	FASTA	200	22,241
	10 20 30 40 50 60 MAHPFTLLVT VGSTLFPSLT SHVLLPTFLS LLQSLGVQRL VVQYGRAELK LQDDVKQTLN 70 80 90 100 110 120 IDSQGDGIGV WSDNDGDRVR DEKQNGMVVE VMRFTNDFEG LVGKSDAVIS HAGSGSILTV 130 140 150 160 170 180 LRRAPPIPLL VVPNRSLMDD HQSELADALY KDGYVMVASV EDLEEKVQPF LKIWPSQAKL 190 200 FPETRKEVFR EVVDDLMGYD

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017342 Genomic DNA. Translation: AAW41985.1. AAEY01000006 Genomic DNA. Translation: EAL22809.1.
RefSeq	XP_569292.1. XP_777456.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KLF2.
Genome annotation databases
GeneID	3256002. 4934141.
GenomeReviews	Gene locus CNB05490 in contig AE017342_GR.
KEGG	cnb:CNBB0300. cne:CNB05490.
Phylogenomic databases
eggNOG	fuNOG11732.
HOGENOM	HBG629711.
OrthoDB	EOG9ZCVN9.
PhylomeDB	Q5KLF2.
Enzyme and pathway databases
BRENDA	2.4.1.141. 2772.
Family and domain databases
InterPro	IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG13_CRYNE

Accession

Primary (citable) accession number: Q5KLF2
Secondary accession number(s): Q55YI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)