Reviewed, UniProtKB/Swiss-Prot Q5KL13 (ATG15_CRYNE)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
Ordered Locus Names:	CNC01140, CNBC6140

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Putative lipase ATG15		PRO_0000317963
Regions
	Topological domain	1 – 5	5	Cytoplasmic By similarity
	Transmembrane	6 – 26	21	Signal-anchor for type II membrane protein
	Topological domain	27 – 522	496	Lumenal By similarity
Sites
	Active site	318	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	302	1	N-linked (GlcNAc...) Potential
	Glycosylation	309	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	80	1	V → M in strain: B-3501A. Ref.1
	Natural variant	439	1	G → E in strain: B-3501A. Ref.1
	Natural variant	494 – 496	3	Missing in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5KL13-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: D140C3E99EABE3FA	FASTA	522	57,210
	10 20 30 40 50 60 MYIPGPLRLS SYLLPFLSSP SPPAQSSPDT RTISFKPVHA HGHAFVDNAS TPTLLFLDQS 70 80 90 100 110 120 PSASLYAHDY PIGAFGDDYV LPRLTSDVLE IRTRKKLIRR PKVRPPRIIS WAQSYRAQAL 130 140 150 160 170 180 HFNGINNNND NSNKSISLPE SWLAPDLANP SDEWSDVEVT VPDLTDRQTV ITLAKMSSNA 190 200 210 220 230 240 YVTPGGAGWY TLNDWNASMP FGWEPDADGL RGHVFADEKN ETVIISIKGT SAGVLGSGGP 250 260 270 280 290 300 TAKNDKFNDN LLFSCCCARV DFSWTPVCDC YAGGYKCGQT CLEDALVSES VYATVGTNLY 310 320 330 340 350 360 NNITYMYPNA TIWLTGHSLG GAVSSLIGLS FGAPAVTYES PGELLPASRL HLPLPPGMPA 370 380 390 400 410 420 NLSGITHVYH TADPIAMGVC NGPYSSCYAA GFAMESKCHT GETILYDTVR VKGWSVDVRT 430 440 450 460 470 480 HRIEEVIDKV LADPWPEEGE GKSGVWEKAV EGWYRAADRV RAALDESVVR DDVNVWWGWG 490 500 510 520 RRGPKRQPGG EDPGWRKHGG VPKPVSEEDC VDCYKWEFGE WN

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS MET-80; GLU-439 AND 494-GLY--ARG-496 DEL. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017343 Genomic DNA. Translation: AAW42129.1. AAEY01000017 Genomic DNA. Translation: EAL21576.1.
RefSeq	XP_569436.1. XP_776223.1.
UniGene	Fne.3396
3D structure databases
SMR	Q5KL13. Positions 164-331, 167-376.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KL13.
Genome annotation databases
GeneID	3256692. 4935280.
GenomeReviews	Gene locus CNC01140 in contig AE017343_GR.
KEGG	cnb:CNBC6140. cne:CNC01140.
Phylogenomic databases
eggNOG	fuNOG04745.
OrthoDB	EOG973R85.
Enzyme and pathway databases
BRENDA	3.1.1.3. 2772.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_CRYNE

Accession

Primary (citable) accession number: Q5KL13
Secondary accession number(s): Q55V69

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)