Reviewed, UniProtKB/Swiss-Prot Q5KKY2 (MCES_CRYNE)

Names and origin

Protein names

Recommended name:
    mRNA cap guanine-N7 methyltransferase
    EC=2.1.1.56
Alternative name(s):
    mRNA (guanine-N(7)-)-methyltransferase
    mRNA cap methyltransferase

Gene names

Name:	ABD1
Ordered Locus Names:	CNC01440, CNBC5820

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	700 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Responsible for methylating the 5'-cap structure of mRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 700	700	mRNA cap guanine-N7 methyltransferase		PRO_0000303907
Regions
	Compositional bias	12 – 356	345	Pro-rich
Natural variations
	Natural variant	368	1	D → N in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5KKY2-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 3940F41384A42767	FASTA	700	77,859
	10 20 30 40 50 60 MVYDPIRDCD VPAPHISAPP LSRSRSPPPP HRTPSASGSL RGLLNDSPPP REPPRAASVH 70 80 90 100 110 120 EDAESHRPKL SNILNDAEPP RQRSMLPPQQ PIHAELEHGR RTSAGSHAAQ LARSPSMSLS 130 140 150 160 170 180 PRSQNQSLPY PSSRPGSAAG SAHPFGYDPR QGTLSPVLSA RRTSEDQPRP TSSSSASGRR 190 200 210 220 230 240 YTEPASQTPA PAPLGSSAYR PRHTSTPGNP SSAYAPRFTP QPTTTPSSPS TSQHTPYTPH 250 260 270 280 290 300 HSAPPRILHY NPHRQSAPSS VLRPIYPDEV AHLRQLAHAN NPLRQKPAAR RYSYSGGRAP 310 320 330 340 350 360 EPTPTPRTSL PGENDHSYFP PQWDDRPSMP PSEVSYGGPP SSTPGAPPSG YSQYPPNWEA 370 380 390 400 410 420 QTPGGNWDGE RPGRLGKRRA RDEEDDEYER NKRVVSGPVA GQAYSKKVTV VAEHYNSRPE 430 440 450 460 470 480 VGVERREFSP IIGLKKFNNW IKSVLIGKFA YRPRGKVLDV GCGKGGDLNK WKQARIALYV 490 500 510 520 530 540 GLDVADQSVQ QAADRYRRMP KPGFDAFFYA HDCFSNPLSD VLSPELQIKD LYDNVTMQFC 550 560 570 580 590 600 MHYAFENAAK ARMMIENVSR YLRRGGIFIG TIPNAELLLQ LPDRDEELRF GNSCYSIQFT 610 620 630 640 650 660 ERRHKGVYGH DYRFYLTDAV EDVPEYLVDW ENFVSLASES GLRLVYKKAF HEILQEEKDS 670 680 690 700 RDFGPLLGKM GVLNEYGESA MDADQWEAAN LYMGFAFEKM

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017343 Genomic DNA. Translation: AAW42167.1. AAEY01000016 Genomic DNA. Translation: EAL21718.1.
RefSeq	XP_569474.1. XP_776365.1.
3D structure databases
HSSP	HSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
SMR	Q5KKY2. Positions 431-700.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5KKY2.
Genome annotation databases
GeneID	3256695. 4935248.
GenomeReviews	Gene locus CNC01440 in contig AE017343_GR.
KEGG	cnb:CNBC5820. cne:CNC01440.
Phylogenomic databases
eggNOG	fuNOG05085.
OrthoDB	EOG9GMWD7.
Enzyme and pathway databases
BRENDA	2.1.1.56. 2772.
Family and domain databases
InterPro	IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCES_CRYNE

Accession

Primary (citable) accession number: Q5KKY2
Secondary accession number(s): Q55VA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)