Reviewed, UniProtKB/Swiss-Prot Q5KKA9 (PMIP1_CRYNE)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase 1
      Short name=MIP 1
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase 1

Gene names

Name:	OCT1
Ordered Locus Names:	CNC03660, CNBC3550

Organism

Cryptococcus neoformans (Filobasidiella neoformans) [Complete proteome]

Taxonomic identifier

5207 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 761		Mitochondrial intermediate peptidase 1		PRO_0000338581
Regions
	Compositional bias	24 – 35	12	Poly-Pro
Sites
	Active site	531	1	By similarity
	Metal binding	530	1	Zinc; catalytic By similarity
	Metal binding	534	1	Zinc; catalytic By similarity
	Metal binding	537	1	Zinc; catalytic By similarity
Natural variations
	Natural variant	110	1	R → H in strain: B-3501A.

Sequences

	Sequence		Length	Mass (Da)
	Q5KKA9-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A4CAE0D603108EFD	FASTA	761	83,290
	10 20 30 40 50 60 MLRLPRITRR ALSSGALAPH FDRPLPPPPP APPAPLCALP PLTAPDALAP LTRRTVRHAD 70 80 90 100 110 120 ALVARIAAAP AHPDPAELRR VVKNLDRLSD VLCGVIDMCE LVRNVHPDPR WVAAAEKTYE 130 140 150 160 170 180 TLCSFMNQLN TSTGLYDALV ATVSHTFPGN PLSPAELRVA QTFLSDFERS GIQLPPGVRA 190 200 210 220 230 240 KFVRHSDNIL SLGRTFLSFA AAGPSADTPI EIPEPEVLLA GLSSKFVASL PRKKRKGPAL 250 260 270 280 290 300 LAPGSWEAQM IGRYADNEEA RRLVYIGSMR EDKDRVYVLE TMLKERAELA HVLGKETWAD 310 320 330 340 350 360 VALSDKMAKT PQNVLQFLTS LATHHRPSAA ADVAALQRLK ALSTVSRTSS QLPTVHAWDR 370 380 390 400 410 420 DHYAEQYAAS LLPNGSLPSI TPYFSVGTAM SGLSHMLSRL YGISFKPVSV AHGEVWHPSV 430 440 450 460 470 480 RRLDVMDEHG KRIGVIYCDL FSRPGKPSAG AAHYTVRCSR RVDDDPSEGD GLPPGWDQHL 490 500 510 520 530 540 GKGMEVQGEA LHGKEGKYQL PIVVLTTDFG TVEESGPALL GWNDLETLFH EMGHAIHSMI 550 560 570 580 590 600 GQTEFHNVSG TRCATDFVEL PSILMEHFIS SPAVLSTFAT HYTTNEPLPI PLIQAHLQLD 610 620 630 640 650 660 QSLKALETHS QILMALLDQK YHSIKHGEQL DSTRVWNELQ SQVGVIPPVR GTAWQTQFGH 670 680 690 700 710 720 LYGYGATYYS YLFDRAIAGK IWSSLFARGR TGPAAANHDP AAAEDILSRE GGEAFKEKVL 730 740 750 760 KWGGGRDPWE MVGDVIGGAE GEQVAKGDEK AMELVGRWMI K

References

[1]

"The genome of the basidiomycetous yeast and human pathogen Cryptococcus neoformans." Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D., Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E., Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A., Fox D.S. , Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C., Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J., Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A., Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E., Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A., Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W., Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W. Science 307:1321-1324(2005) [PubMed: 15653466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: B-3501A and JEC21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017343 Genomic DNA. Translation: AAW42284.1. AAEY01000013 Genomic DNA. Translation: EAL22217.1.
RefSeq	XP_569591.1. XP_776864.1.
3D structure databases
SMR	Q5KKA9. Positions 45-738.
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	3256482. 4935020.
GenomeReviews	Gene locus CNC03660 in contig AE017343_GR.
KEGG	cnb:CNBC3550. cne:CNC03660.
Phylogenomic databases
eggNOG	fuNOG07066.
HOGENOM	HBG562792.
OMA	PNPAHFT.
OrthoDB	EOG9DZ3BN.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP1_CRYNE

Accession

Primary (citable) accession number: Q5KKA9
Secondary accession number(s): Q55VY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)